Nutrition Research and Practice

The Korean Nutrition Society (한국영양학회)
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Protective role of oligonol from oxidative stress-induced inflammation in C6 glial cell

  • Ahn, Jae Hyun (Department of Food Science and Nutrition, Pusan National University) ;
  • Choi, Ji Won (Department of Food Science and Nutrition, Pusan National University) ;
  • Choi, Ji Myung (Department of Food Science and Nutrition, Pusan National University) ;
  • Maeda, Takahiro (Amino Up Chemical Co., Ltd.) ;
  • Fujii, Hajime (Amino Up Chemical Co., Ltd.) ;
  • Yokozawa, Takako (Institute of Natural Medicine, University of Toyama) ;
  • Cho, Eun Ju (Department of Food Science and Nutrition, Pusan National University)
  • Received : 2014.05.26
  • Accepted : 2014.08.27
  • Published : 2015.04.01
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Abstract

BACKGROUND/OBJECTIVES: Natural products or active components with a protective effect against oxidative stress have attracted significant attention for prevention and treatment of degenerative disease. Oligonol is a low molecular weight polyphenol containing catechin-type monomers and oligomers derived from Litchi chinensis Sonn. We investigated the protective effect and its related mechanism of oligonol against oxidative stress. MATERIALS/METHODS: Oxidative stress in C6 glial cells was induced by hydrogen peroxide ($H_2O_2$) and the protective effects of oligonol on cell viability, nitric oxide (NO) and reactive oxygen species (ROS) synthesis, and mRNA expression related to oxidative stress were determined. RESULTS: Treatment with oligonol inhibited NO and ROS formation under cellular oxidative stress in C6 glial cells. In addition, it recovered cell viability in a dose dependent-manner. Treatment with oligonol also resulted in down-regulated mRNA expression related to oxidative stress, nuclear factor kappa-B (NF-${\kappa}B$) p65, cyclooxygenase-2 (COX-2), and inducible nitric oxide synthase (iNOS), compared with the control group treated with $H_2O_2$. In particular, expression of NF-${\kappa}B$ p65, COX-2, and iNOS was effectively reduced to the normal level by treatment with $10{\mu}g/mL$ and $25{\mu}g/mL$ of oligonol. CONCLUSIONS: These results indicate that oligonol has protective activity against oxidative stress-induced inflammation. Oligonol might be a promising agent for treatment of degenerative diseases through inhibition of ROS formation and NF-${\kappa}B$ pathway gene expression.

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References

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