International Journal of Oral Biology

The Korean Academy of Oral Biology (대한구강생물학회)
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Osteocalcin Expression and Mineralization in Developing Tooth of Xenopus laevis

  • Park, Jung Hoe (Department of Oral Anatomy, School of Dentistry, and Institute of Oral Biosciences, Chonbuk National University) ;
  • Kwon, Ki-Tak (Department of Oral Anatomy, School of Dentistry, and Institute of Oral Biosciences, Chonbuk National University) ;
  • Park, Byung Keon (Department of Oral Anatomy, School of Dentistry, and Institute of Oral Biosciences, Chonbuk National University) ;
  • Lee, Young-Hoon (Department of Oral Anatomy, School of Dentistry, and Institute of Oral Biosciences, Chonbuk National University)
  • Received : 2015.01.20
  • Accepted : 2015.02.10
  • Published : 2015.03.31
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Abstract

Osteocalcin (OC) is the most abundant noncollagenous protein of extracellular matrix in the bone. In an OC deficient mouse, bone formation rates are increased in cancellous and cortical bones. OC is known as a negative regulator of mineral apposition. OC is also expressed in the tooth of the rat, bovine, and human. However, little is known about OC during tooth development in Xenopus. The purpose of this study is to compare the expression of OC with mineralization in the developing tooth of Xenopus, by using von Kossa staining and in situ hybridization. At stage 56, the developmental stage of tooth germ corresponds to the cap stage, and an acellular zone was apparent between the dental papilla and the enamel organ. From stage 57, calcium deposition was revealed by von Kossa staining prior to OC expression, and the differentiated odontoblasts forming predentin were located at adjoining predentin. At stage 58, OC transcripts were detected in the differentiated odontoblasts. At stage 66, OC mRNA was expressed in the odontoblasts, which was aligned in a single layer at the periphery of the pulp. These findings suggest that OC may play a role in mineralization and odontogenesis of tooth development in Xenopus.

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References

  1. Smith MM, Coates MI. Evolutionary origins of the vertebrate dentition: phylogenetic patterns and developmental evolution. Eur J Oral Sci. 1998;106 Suppl 1:482-500. https://doi.org/10.1111/j.1600-0722.1998.tb02212.x
  2. Assaraf-Weill N, Gasse B, Al-Hashimi N, Delgado S, Sire JY, Davit-Beal T. Conservation of amelogenin gene expression during tetrapod evolution. J Exp Zool B Mol Dev Evol. 2013;320:200-209. doi: 10.1002/jez.b.22494.
  3. Davit-Beal T, Chisaka H, Delgado S, Sire JY. Amphibian teeth: current knowledge, unanswered questions, and some directions for future research. Biol Rev Camb Philos Soc. 2007;82:49-81. doi: 10.1111/j.1469-185X.2006.00003.x.
  4. Handrigan GR, Richman JM. Unicuspid and bicuspid tooth crown formation in squamates. J Exp Zool B Mol Dev Evol. 2011;316:598-608. doi: 10.1002/jez.b.21438.
  5. Stock DW. Zebrafish dentition in comparative context. J Exp Zool B Mol Dev Evol. 2007;308:523-549. doi: 10.1002/jez.b.21187.
  6. Lee YH, Williams A, Hong CS, You Y, Senoo M, Saint-Jeannet JP. Early development of the thymus in Xenopus laevis. Dev Dyn. 2013;242:164-178. doi: 10.1002/dvdy.23905.
  7. Lee YH, Saint-Jeannet JP. Cardiac neural crest is dispensable for outflow tract septation in Xenopus. Development. 2011;138:2025-2034. doi: 10.1242/dev.061614.
  8. Thesleff I, Vainio S, Jalkanen M. Cell-matrix interactions in tooth development. Int J Dev Biol. 1989;33:91-97.
  9. Ruch JV, Lesot H, Begue-Kirn C. Odontoblast differentiation. Int J Dev Biol. 1995;39:51-68.
  10. Kawasaki K. The SCPP gene repertoire in bony vertebrates and graded differences in mineralized tissues. Dev Genes Evol. 2009;219:147-157. doi:10.1007/s00427-009-0276-x.
  11. Goldberg M, Kulkarni AB, Young M, Boskey A. Dentin: structure, composition and mineralization. Front Biosci. 2011;3:711-735. https://doi.org/10.2741/e281
  12. Hauschka PV, Reddi AH. Correlation of the appearance of gamma-carboxyglutamic acid with the onset of mineralization in developing endochondral bone. Biochem Biophys Res Commun. 1980;92:1037-1041. https://doi.org/10.1016/0006-291X(80)90806-2
  13. Otawara Y, Price PA. Developmental appearance of matrix GLA protein during calcification in the rat. J Biol Chem. 1986;261:10828-10832.
  14. Hashimoto F, Kobayashi Y, Kobayashi ET, Sakai E, Kobayashi K, Shibata M, Kato Y, Sakai H. Expression and localization of MGP in rat tooth cementum. Arch Oral Biol. 2001;46:585-592. doi: http://dx.doi.org/10.1016/S0003-9969(01)00022-X.
  15. Hauschka PV, Lian JB, Gallop PM. Direct identification of the calcium-binding amino acid, gamma-carboxyglutamate, in mineralized tissue. Proc Natl Acad Sci USA. 1975;72: 3925-3929. https://doi.org/10.1073/pnas.72.10.3925
  16. Price PA, Otsuka AS, Poser JW, Kristaponis J, aman N. Characterization of a gamma-carboxyglutamic acidcontaining protein from bone. Proc Nail Acad Sci USA. 1976;73:1447-1451. https://doi.org/10.1073/pnas.73.5.1447
  17. Yamamoto T, Takemura T, Kitamura Y, Nomura S. Site-specific expression of mRNAs for osteonectin, osteocalcin, and osteopontin revealed by in situ hybridization in rat periodontal ligament during physiological tooth movement. J Histochem Cytochem. 1994;42:885-896. https://doi.org/10.1177/42.7.8014472
  18. Ducy P, Desbois C, Boyce B, Pinero G, Story B, Dunstan C, Smith E, Bonadio J, Goldstein S, Gundberg C, Bradley A, Karsenty G. Increased bone formation in osteocalcindeficient mice. Nature. 1996;382:448-452. https://doi.org/10.1038/382448a0
  19. de Vries IG, Coomans D, Wisse E. Immunocytochemical localization of osteocalcin in human and bovine teeth. Calcif Tissue Int. 1988;43:128-130. https://doi.org/10.1007/BF02555159
  20. Bronckers AL, D'Souza RN, Butler WT, Lyaruu DM, van Dijk S, Gay S, Woltgens JH. Dentin sialoprotein: biosynthesis and developmental appearance in rat tooth germs in comparison with amelogenins, osteocalcin and collagen type-I. Cell Tissue Res. 1993;272:237-247. https://doi.org/10.1007/BF00302729
  21. Papagerakis P, Berdal A, Mesbah M, Peuchmaur M, Malaval L, Nydegger J, Simmer J, Macdougall M. Investigation of osteocalcin, osteonectin, and dentin sialophosphoprotein in developing human teeth. Bone. 2002;30:377-385. doi: http://dx.doi.org/10.1016/S8756-3282(01)00683-4.
  22. Ortiz-Delgado JB, Simes DC, Gavaia P, Sarasquete C, Cancela ML. Osteocalcin and matrix GLA protein in developing teleost teeth: identification of sites of mRNA and protein accumulation at single cell resolution. Histochem Cell Biol. 2005;124:123-130. doi: 10.1007/s00418-005-0015-y.
  23. Ge Y, Kong Z, Guo Y, Tang W, Guo W, Tian W. The role of odontogenic genes and proteins in tooth epithelial cells and their niche cells during rat tooth root development. Arch Oral Biol. 2013;58:151-159. doi: 10.1016/j. archoralbio. 2012.04.017.
  24. Nieuwkoop PD, Faber J. Normal table of Xenopus laevis (Daudin). Amsterdam: North Holland Publishing Company, 1967.
  25. Lemaire P, Gurdon JB. A role for cytoplasmic determinants in mesoderm patterning: cell-autonomous activation of the goosecoid and Xwnt-8 genes along the dorsoventral axis of early Xenopus embryos. Development. 1994;120:1191-1199.
  26. Huysseune A, Sire JY. Evolution of patterns and processes in teeth and tooth-related tissues in non-mammalian vertebrates. Eur J Oral Sci. 1998;106 Suppl 1:437-481. https://doi.org/10.1111/j.1600-0722.1998.tb02211.x
  27. Johanson Z, Smith MM. Origin and evolution of gnathostome dentitions: a question of teeth and pharyngeal denticles in placoderms. Biol Rev Camb Philos Soc. 2005;80:303-345. doi: http://dx.doi.org/10.1017/ S1464793104006682.
  28. Shaw JP. The time scale of tooth development and replacement in Xenopus laevis (Daudin) J Anat. 1979;129: 323-342.