Journal of Korean Society of Environmental Engineers (대한환경공학회지)

Korean Society of Environmental Engineers (대한환경공학회)
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Determination of Trace Amounts of Formaldehyde in Water Using High Performance Liquid Chromatography and Acetylacetone as a Derivative Reagent

아세틸아세톤 유도체화 시약과 HPLC를 이용한 미량 포름알데하이드 수질분석

  • Lee, Ki-Chang (Gyeongsangbuk-do Institute of Health and Environment) ;
  • Park, Jae-Hyung (Gyeongsangbuk-do Institute of Health and Environment) ;
  • Lee, Wontae (Department of Environmental Engineering, Kumoh National Institute of Technology)
  • 이기창 (경상북도보건환경연구원) ;
  • 박재형 (경상북도보건환경연구원) ;
  • 이원태 (금오공과대학교 환경공학전공)
  • Received : 2015.01.08
  • Accepted : 2015.02.11
  • Published : 2015.02.28
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Abstract

A simple analytical method to quantify formaldehyde in water at lower levels (${\mu}g/L$) was developed using a high performance liquid chromatography (HPLC) and acetylacetone as a derivative reagent. Unlike conventional methods, no extraction and/or concentration were required. The derivative reagent was added into samples and reacted for 30 minutes at $80^{\circ}C$ prior to the analysis of formaldehyde using HPLC. The method detection limit and the limit of quantification for this method were 1.6 and $5.0{\mu}g/L$, respectively. This method also achieved high precision (0.6-3.0%) and accuracy (91.6-106.3%). The recovery rates for various environmental samples ranged from 92.0 to 115.2%.

수중에 존재하는 포름알데하이드(formaldehyde)를 ${\mu}g/L$ 단위 농도까지 정량할 수 있는 간단한 분석법을 개발하였다. 시료 전처리시 유도체화 시약으로 아세틸아세톤(acetylacetone)을 사용하였고, 기존 분석방법과 달리 추출 및 농축과정을 거치지 않는 것이 장점이다. 시료에 유도체화 시약을 첨가하고 $80^{\circ}C$에서 30분 반응시킨 후 HPLC를 이용하여 포름알데하이드를 분석하였다. 본 방법에 대한 정도보증 결과, 방법검출한계 및 정량한계는 각각 1.6, $5.0{\mu}g/L$로 기존 분석법에 비하여 낮았다. 정밀도 및 정확도는 각각 0.6~3.0%, 91.6~106.3%로 우수하였다. 또한 다양한 환경시료에 대한 회수율 검증에서도 92.0~115.2%로 양호하게 나타났다.

Keywords

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