Journal of the Korean Wood Science and Technology

The Korean Society of Wood Science & Technology (한국목재공학회)
권호 표지
DOI QR코드

DOI QR Code

Anti-apoptotic Effect of Steam Exploded Quercus variabilis

  • Jo, Jong-Soo (Department of Interior Materials Engineering, Gyeongnam National University of Science and Technology) ;
  • Jung, Ji Young (Division of Environmental Forest Science, Institute of Agriculture & Life Scienece, Gyeongsang National University) ;
  • Nam, Jeong Bin (Division of Environmental Forest Science, Institute of Agriculture & Life Scienece, Gyeongsang National University) ;
  • Park, Hyung Bin (Department of Orthopaedic Surgery, School of Medicine, Gyeongsang National University) ;
  • Yang, Jae-Kyung (Division of Environmental Forest Science, Institute of Agriculture & Life Scienece, Gyeongsang National University)
  • Received : 2014.09.29
  • Accepted : 2015.02.05
  • Published : 2015.03.25
Download PDF
⟨ Previous Next ⟩

Abstract

We hypothesized that the extract from steam exploded Q. variabilis might be cytoprotective for tenofibroblasts cells during oxidative stress. In the present study, the extracts obtained from steam exploded (severity log Ro 4.68) Q. variabilis contained high quantities of phenolics and flavonoids contents. Also, the extracts appear to have, on these tenofibroblasts, a protective effect against oxidative stress. Tenofibroblasts cells incubated with the extracts and stressed with $H_2O_2$ showed an increase in cell viability by MTT assay. The extracts is found to inhibit $H_2O_2$-induced apoptosis in tenofibroblasts cells, as shown by Annexin V/PI double staining analysis. Western blot data showed that in the extracts/$H_2O_2$-treated cells, the extracts inhibited the $H_2O_2$-dependent phosphorylation of ERK and p38. From these results, it is suggested that the extracts showed the protective effect on $H_2O_2$-mediated oxidative stress. The main chemical compounds of the extract was identified as 1,8-cineole by GC-MS analysis. The anti-apoptosis activity is accordingly believed to be attributable to the 1,8-cineole.

Keywords

References

  1. Ando, S., Arai, I., Kiyoto, K., Hanai, S. 1986. Identification of aromatic monomers in steamexploded poplar and their influences on ethanol fermentation by Saccharomyces cerevisiae. Journal of Fermentation Technology, 64, 567-570. https://doi.org/10.1016/0385-6380(86)90084-1
  2. Castro, E., Conde, E., Moure, A., Falque', E., Cara, C., Ruiz, E., Dominguez, H. 2008. Antioxidant activity of liquors from steam explosion of Olea europea wood. Wood Science Technology 42: 579-592. https://doi.org/10.1007/s00226-007-0169-y
  3. Chen, J., Wang, A., Xu, J., Zheng, M. 2010. In chronic lateral epicondylitis, apoptosis and autophagic cell death occur in the extensor carpi radialis brevis tendon. Journal of Shoulder and Elbow Surgery. 19: 355-362. https://doi.org/10.1016/j.jse.2009.07.064
  4. Conde, E., Cara, C., Moure, A., Ruiz, E., Castro, E., Dominguez, H. 2009. Antioxidant activity of the phenolic compounds released by hydrothermal treatments of olive tree pruning. Food Chemistry. 114(4): 806-812. https://doi.org/10.1016/j.foodchem.2008.10.017
  5. Cruz, J. M., Dominguez, J. M., Dominguez, H., Parajo', J. C. 1999. Solvent extraction of hemicellulosic wood hydrolysates: a procedure useful for obtaining both detoxified fermentation media and polyphenols with antioxidant activity. Food Chemistry. 67: 147-153. https://doi.org/10.1016/S0308-8146(99)00106-5
  6. Fenton, H.J.H. 1984. Oxidation of tartaric acid in the presence of iron. Journal of the Chemical Society. 65: 899-910.
  7. Gurhan, G., Ezer, N. 2004. Halk arasinda hemoroid tedavisinde kullanilan bitkiler. Hacettepe University Journal of the Faculty of Pharmacy. 24: 37-55.
  8. Han, S.Y., Li, H.X., Ma, X., Zhang, K., Ma, Z.Z., Tu, P.F. 2009. Protective effects of purified safflower extract on myocardial ischemia in vivo and in vitro. Phytomedicine. 16: 694-702. https://doi.org/10.1016/j.phymed.2009.02.019
  9. Han, S.-Y., Lia, H.-X., Ma, X., Zhang, K., Ma, Z.-Z., Tu, P.-F. 2009. Protective effects of purified safflower extract on myocardial ischemia in vivo and in vitro. Phytomedicine. 16(8): 694-702. https://doi.org/10.1016/j.phymed.2009.02.019
  10. Hendry, E.R., Worthington, T., Conway, B.R., Lambert, P.A. 2009. Antimicrobial efficacy of eucalyptus oil and 1,8-cineole alone and in combination with chlorhexidine digluconate against microorganisms grown in planktonic and biofilm biofilm cultures. Journal of Antimicrobial Chemotherapy 64: 1219-1225. https://doi.org/10.1093/jac/dkp362
  11. Jacobson, M.D. 1996. Reactive oxygen species and programmed cell death. Trends in biochemical sciences. 21: 83-86. https://doi.org/10.1016/S0968-0004(96)20008-8
  12. Kerr, J.F., Wyllie, A.H., Currie, A.R. 1972. Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics. British Journal of Cancer. 26: 239-257 https://doi.org/10.1038/bjc.1972.33
  13. Klocke, J.A., Darlington, M.V., Balandrin, M.F. 1987. "8-Cineole (Eucalyptol), a Mosquito Feeding and Ovipositional Repellent from Volatile Oil of Hemizonia fitchii (Asteraceae)". Journal of Chemical Ecology 13 (12): 2131. https://doi.org/10.1007/BF01012562
  14. Kurosumi, A., Kobayashi, F., Mtui, G., Nakamura, Y. 2006. Development of optimal culture method of Sparassis crispa mycelia and a new extraction method of antineoplastic constituent. Biochemical Engineering Journal 30: 109-113. https://doi.org/10.1016/j.bej.2006.02.004
  15. Kurosumi, A., Sasaki, C., Kumada, K., Kobayashi, F., Mtui, G., Nakamura, Y. 2007. Novel extraction method of antioxidant compounds from Sasa palmata (Bean) Nakai using steam explosion. Process Biochemistry. 42: 1449-1453. https://doi.org/10.1016/j.procbio.2007.06.007
  16. Kwak, M.K., Cho, J.M., Huang, B., Shin, S., Kensler, T.W. 2007. Role of increased expression of the proteasome in the protective effects of sulforaphane against hydrogen peroxide-mediated cytotoxicity in murine neuroblastoma cells. Free Radical Biology and Medicine. 43: 809-817 https://doi.org/10.1016/j.freeradbiomed.2007.05.029
  17. Larsson, S., Palmqvist, E., Hahn-Hagerdal, B., Tenborg, C., Stenberg, K., Zacchi, G., Nilvebrant, N.O. 1999. The generation of fermentation inhibitors during dilute acid hydrolysis of softwood. Enzyme Microbial Technology, 24: 151-159 https://doi.org/10.1016/S0141-0229(98)00101-X
  18. Nam, J.B., Park, H.B., Jung, J.Y., Yang, J,-K. 2014. Protective Effect of Oak Extracts on Oxidative Stress Induced by Hydrogen Peroxide. Journal of the Korean Wood Science and Technology 42(5): 523-532. https://doi.org/10.5658/WOOD.2014.42.5.523
  19. Park, G.H., Jang, D.S., Oh, M.S. 2012. Juglans mandshurica lear extract protects skin fibroblasts from damage by regulating the oxidative defense system. Biochemical and Biophysical Research Communications. 421: 343-348 https://doi.org/10.1016/j.bbrc.2012.04.013
  20. Park, H.B., Hah, Y.S., Yang, J.W., Nam, J.B., Cho, S.H., Jeong, S.T. 2010. Antiapoptotic effects of anthocyanins on rotator cuff tenofibroblasts. Journal of Orthopaedic Research. 28: 1162-1169 https://doi.org/10.1002/jor.21097
  21. Romussi, G., Barbieri, G., Caviglioli, G. 1994. Triterpene saponins and flavonoids from Quercus imbricaria. Pharmazie. 49: 703-704.
  22. Scott, A., Khan, K.M., Duronio, V. 2005. IGF-I activates PKB and prevents anoxic apoptosis in Achilles tendon cells. Journal of Orthopaedic Research. 23: 1219-1225. https://doi.org/10.1016/j.orthres.2004.12.011
  23. Sezik, E., Yesilada, E., Honda, G., Takashi, Y., Takeda, Y., Tanaka, T. 2001. Traditional medicine in Turkey X. Folk medicine in Central Anatolia. Journal of Ethnopharmacology. 75: 95-115. https://doi.org/10.1016/S0378-8741(00)00399-8
  24. Sfara, V., Zerba, E.N., Alzogaray, R.A. 2009 "Fumigant Insecticidal Activity and Repellent Effect of Five Essential Oils and Seven Monoterpenes on First-Instar Nymphs of Rhodnius prolixus". Journal of Medical Entomology 46 (3):511-515. https://doi.org/10.1603/033.046.0315
  25. Singh, H.P., Mittal, S., Kaur, S., Batish, D.R., Kohli, R.K. 2009. Characterization and antioxidant activity of essential oils from fresh and decaying leaves of eucalyptus tereticornis. J. Agric. Food Chem. 57: 6962-6966. https://doi.org/10.1021/jf9012407
  26. Skutek, M., van Griensven, M., Zeichen, J., Brauer, N., Bosch, U. 2003. Cyclic mechanical stretching of human patellar tendon fibroblasts: activation of JNK and modulation of apoptosis. Knee Surgery Sports Traumatology Arthroscopy. 11: 122-129 https://doi.org/10.1007/s00167-002-0322-y
  27. Sohretoglu, D., Sakar, M.K. 2004. Polyphenolic constituents and biological activities of Quercus species. Ankara University, Faculty of Pharmacy. 33: 183-215.
  28. Sohretoglu, D., Ekizoglu, M., Kilic, E., Sakar, M.K. 2007. Antibacterial and antifungal activities of some Quercus species growing in Turkey. FABAD Journal of Pharmaceutical sciences. 32: 127-130.
  29. Sohretoglu, D., Sabuncuoglu, S., Harput, U.S. 2012. Evaluation of antioxidative, protective effect against H2O2 induced cytotoxicity, and cytotoxic activities of three different Quercus species. Food and Chemical Toxicology. 50: 141-146. https://doi.org/10.1016/j.fct.2011.10.061
  30. Sun, X.F., Xu, F., Sun, R.C., Wang, Y.X., Fowler, P., Baird, M.S. 2004. Characteristics of degraded lignins obtained from steam exploded wheat straw. Polym Degrad Stabil 86: 245-256. https://doi.org/10.1016/j.polymdegradstab.2004.05.003
  31. Taylor, J.D., Yu, E.K.C. 1995. Continuous Steam Explosion. Chemtech-American Chernical Society 2: 38-41.
  32. Thompson, C.B. 1995. Apoptosis in the pathogenesis and treatment of disease. Science. 267: 1456-1462. https://doi.org/10.1126/science.7878464
  33. Tuoheti, Y., Itoi, E., Pradhan, R.L., Wakabayashi, I., Takahashi, S., Minagawa, H., Okada, K., Shimada, Y. 2005. Apoptosis in the supraspinatus tendon with stage II subacromial impingement. Journal of Shoulder and Elbow Surgery. 14: 535-541. https://doi.org/10.1016/j.jse.2005.01.001
  34. Wood, K.A., Youle, R.J. 1994. Apoptosis and free radicals. Annals of the New York Academy of Sciences. 17: 400-407
  35. Yuan, J., Murrell, G.A., Wei, A.Q., Wang, M.X. 2002. Apoptosis in rotator cuff tendinopathy. Journal of Orthopaedic Research. 20: 1372-1379 https://doi.org/10.1016/S0736-0266(02)00075-X