Korean Journal of Environmental Agriculture (한국환경농학회지)

The Korean Society of Environmental Agriculture (한국환경농학회)
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α-Glucosidase Inhibitory Effect of Vicine and α-Eleostearic Acid from the Seeds of Momordica charantia

여주씨에서 분리한 Vicine과 α-Eleostearic Acid의 α-glucosidase 저해효과

  • Yuk, Heung Joo (Department of Agro-food Resources, National Academy of Agricultural Science, Rural Development Administration) ;
  • Noh, Geon Min (Department of Agro-food Resources, National Academy of Agricultural Science, Rural Development Administration) ;
  • Choe, Jeong Sook (Department of Agro-food Resources, National Academy of Agricultural Science, Rural Development Administration) ;
  • Kwon, Oh Kyung (Advanced Food Materials Research Institute, Kolmar BNH Inc.) ;
  • Hong, Su Young (Advanced Food Materials Research Institute, Kolmar BNH Inc.) ;
  • Kang, Sang Soo (Department of Anatomy, School of Medicine, Gyeongsang National University) ;
  • Cho, Kye Man (Department of Food Science, Gyeongnam National University of Science and Technology) ;
  • Park, Dong Sik (Department of Agro-food Resources, National Academy of Agricultural Science, Rural Development Administration)
  • 육흥주 (농촌진흥청 국립농업과학원 농식품자원부) ;
  • 노건민 (농촌진흥청 국립농업과학원 농식품자원부) ;
  • 최정숙 (농촌진흥청 국립농업과학원 농식품자원부) ;
  • 권오경 (콜마비앤에이치(주)) ;
  • 홍수영 (콜마비앤에이치(주)) ;
  • 강상수 (경상대학교 의학전문대학원 해부학교실) ;
  • 조계만 (경남과학기술대학교 식품과학부) ;
  • 박동식 (농촌진흥청 국립농업과학원 농식품자원부)
  • Received : 2015.02.13
  • Accepted : 2015.03.18
  • Published : 2015.03.31
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Abstract

BACKGROUND: Momordica charantia (MC) have been used in various parts of the world to treat diabetes. Despite the highest effects of phytochemicals than any other part of the plant, the main components of seed of MC grown in Korea and their properties have not been studied extensively. METHODS AND RESULTS: The seeds of MC were extracted into five different polarity solvents: 30%, 50%, 70%, 95% ethanol and distilled water. The 95% ethanol extract showed the most potent inhibition ($IC_{50}=88.7{\mu}g/mL$) against ${\alpha}$-glucosidase. To investigate the compounds responsible for this effect, activity guided fractionation of MC seeds by chromatography yielded two phytochemicals which were confirmed as vicine (1) and ${\alpha}$-eleostearic acid (2) based on their NMR and ESI-MS spectroscopic data. Among them, ${\alpha}$-eleostearic acid (2) possessed potent ${\alpha}$-glucosidase inhibitory activities with $IC_{50}$ values from $32.4{\mu}g/mL$. CONCLUSION: Collectively, the results from this present study strongly suggest that both extract and ${\alpha}$-eleostearic acid (2) have potent ${\alpha}$-glucosidase inhibitory activity. Furthermore, ${\alpha}$-eleostearic acid (2) as the most active ${\alpha}$-glucosidase inhibitor was proven to be present in high quantities in the MC seeds by a HPLC chromatogram.

최근 들어 여주의 혈당개선의 약리학적 효능이 보고됨에 따라 기능성 채소로 각광받고 있다. 주로 여주 과실만이 식용으로 쓰이지만 여주씨 또한 다양한 가공과정을 거칠 경우(건여주 혹은 건조분말, 환, 엑기스 등) 필연적으로 함께 섭취되기도 한다. 본 연구에서는 과실이외의 특히, 연구결과가 미비한 여주씨에 대해 생리활성물질의 구명 및 혈당개선의 효과에 대한 연구를 수행하였다. 다양한 추출물 및 단일 활성성분의 혈당개선 in vitro검증법인 ${\alpha}$-glucosidase에 대한 저해효과를 측정하였고, 활성을 나타내는 추출물 또는 활성성분과의 상관관계를 구명하였다. 구체적으로는 95% 에탄올 추출물 및 비극성 용매 등에 다량 추출되는 생리활성물질인 ${\alpha}$-eleostearic acid는 우수한 저해효과를 나타내었다($IC_{50}=43.4{\mu}g/mL$). 또한 효소저해제의 함량을 HPLC를 통한 정량분석을 수행함으로써 여주씨에 함유된 기능성성분의 혈당개선 식 의약 소재로의 응용가능성을 연구하였다.

Keywords

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