BMB Reports

생화학분자생물학회 (Korean Society for Biochemistry and Molecular Biology)
권호 표지
DOI QR코드

DOI QR Code

The effects of PEP-1-FK506BP on dry eye disease in a rat model

  • Kim, Dae Won (Department of Biochemistry and Molecular Biology, Research Institute of Oral Sciences, College of Dentistry, Gangnung-Wonju National University) ;
  • Lee, Sung Ho (R&D center, Lumieye Genetics Co., Ltd.) ;
  • Ku, Sae Kwang (Department of Anatomy and Histology, College of Korean Medicine, Daegu Haany University) ;
  • Lee, Ji Eun (Department of Anatomy and Histology, College of Korean Medicine, Daegu Haany University) ;
  • Cha, Hyun Ju (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University) ;
  • Youn, Jong Kyu (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University) ;
  • Kwon, Hyeok Yil (Department of Physiology, College of Medicine, Hallym University) ;
  • Park, Jong Hoon (Department of Biological Sciences, Sookmyung Women's University) ;
  • Park, Eun Young (Department of Biological Sciences, Sookmyung Women's University) ;
  • Cho, Sung-Woo (Department of Biochemistry and Molecular Biology, University of Ulsan College of Medicine) ;
  • Han, Kyu Hyung (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University) ;
  • Park, Jinseu (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University) ;
  • Eum, Won Sik (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University) ;
  • Choi, Soo Young (Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University)
  • 투고 : 2014.06.05
  • 심사 : 2014.07.01
  • 발행 : 2015.03.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

As FK506 binding proteins (FK506BPs) are known to play an important role in the regulation of a variety of biological processes related to cell survival, this study was designed to examined the protective effects of FK506 binding protein 12 (FK506BP) on low humidity air flow induced dry eye in a rat model using transduced PEP-1-FK506BP. After the topical application of PEP-1-FK506BP, tear volumes were markedly increased and significant prevention of cornea damage was observed compared with dry eye rats. Further, immunohistochemical analysis demonstrated that PEP-1-FK506BP markedly prevented damage to the cornea, the bulbar conjunctiva, and the palpebral conjunctiva epithelial lining compared with dry eye rats. In addition, caspase-3 and PARP expression levels were found to be decreased. These results demonstrated that topical application of PEP-1-FK506BP significantly ameliorates dry eye injury in an animal model. Thus, we suggest that PEP-1-FK506BP can be developed as a new ophthalmic drop to treat dry eye diseases.

키워드

참고문헌

  1. Nakamura S, Shibuya M, Nakashima H, Imagawa T, Uehara M and Tsubota K (2005) D-β-hydroxybutyrate protects against corneal epithelial disorders in a rat dry eye model with jogging board. Invest Ophthalmol Vis Sci 46, 2679-2387
  2. Chen W, Zhang X, Zhang J et al (2008) A murine model of dry eye induced by an intelligently controlled environmental system. Invest Ophthalmol Vis Sci 49, 1386-1391 https://doi.org/10.1167/iovs.07-0744
  3. Chen W, Zhang X, Liu M et al (2009) Trehalose protects against ocular surface disorders in experimental murine dry eye through suppression of apoptosis. Exp Eye Res 89, 311-318 https://doi.org/10.1016/j.exer.2009.03.015
  4. Schaumberg DA, Sullivan DA and Dana MR (2002) Epidemiology of dry eye syndrome. Ade Exp Med Biol 506, 989-998 https://doi.org/10.1007/978-1-4615-0717-8_38
  5. Schaumberg DA, Buring JE, Sullivan DA and Dana MR (2001) Hormone replacement therapy and dry eye syndrome. JAMA 286, 2114-2119 https://doi.org/10.1001/jama.286.17.2114
  6. Khaw PT, Shah P and Elkington AR (2004) Injury to the eye. BMJ 328, 36-38 https://doi.org/10.1136/bmj.328.7430.36
  7. Behrens A, Doyle JJ, Stern L et al (2006) Dysfunctional tear syndrome study group. Dysfunctional tear syndrome: a Delphi approach to treatment recommendations. Cornea 25, 900-907 https://doi.org/10.1097/01.ico.0000214802.40313.fa
  8. Gupta H, Jain S, Mathur R, Mishra P, Mishra AK and Velpandian T (2007) Sustained ocular drug delivery from a temperature and pH triggered novel in situ gel system. Drug Deliv 14, 507-515 https://doi.org/10.1080/10717540701606426
  9. Hosseini H and Nejabat M (2007) A potential therapeutic strategy for inhibition of corneal neovascularization with new anti-VEGF agents. Med Hypotheses 68, 799-801 https://doi.org/10.1016/j.mehy.2006.06.063
  10. Maitchouk DY, Beuerman RW, Ohta T, Stern M and Varnell RJ (2000) Tear production after unilateral removal of the main lacrimal gland in squirrel monkeys. Arch Ophthalmol 118, 246-252 https://doi.org/10.1001/archopht.118.2.246
  11. Fujihara T, Murakami T, Fujita H, Nakamura M and Nakata K (2001) Improvement of corneal barrier function by the P2Y(2) agonist INS365 in a rat dry eye model. Invest Ophthalmol Vis Sci 42, 96-100
  12. Dursun D, Wang M, Monroy D et al (2002) A mouse model of keratoconjunctivitis sicca. Invest Ophthalmol Vis Sci 43, 632-638
  13. Rolando M and Refojo MF (1983) Tear evaporimeter for measuring water evaporation rate from the tear film under controlled conditions in humans. Exp Eye Res 36, 25-33 https://doi.org/10.1016/0014-4835(83)90086-6
  14. Romano S, D’Angelillo A, Pacelli R et al (2010) Role of FK506-binding protein 51 in the control of apoptosis of irradiated melanoma cells. Cell Death Differ 17, 145-157 https://doi.org/10.1038/cdd.2009.115
  15. Robson T and James IJ (2012) The therapeutic and diagnostic potential of FKBPL: a novel anticancer protein. Drug Discov Today 17, 544-548 https://doi.org/10.1016/j.drudis.2012.01.002
  16. Kay JE (1996) Structure-function relationships in the FK506-binding protein (FKBP) family of peptidylprolyl cis-trans isomerases. Biochem J 314, 361-385 https://doi.org/10.1021/bi9522312
  17. Ivery MT (2000) Immunophilins: switched on protein binding domains? Med Res Rev 20, 452-484 https://doi.org/10.1002/1098-1128(200011)20:6<452::AID-MED2>3.0.CO;2-6
  18. Kim SY, Jeong HJ, Kim DW et al (2011) Transduced PEP-1-FK506BP inhibits the inflammatory response in the Raw 264.7 cells and mouse models. Immunobiol 216, 771-781 https://doi.org/10.1016/j.imbio.2010.12.008
  19. Kim SY, Sohn EJ, Kim DW et al (2011) Transduced PEP-1-FK506BP ameliorates atopic dermatitis in NC/Nga mice. J Invest Dermatol 131, 1477-1485 https://doi.org/10.1038/jid.2011.49
  20. Kim DW, Lee SH, Ku SK et al (2013) Transduced PEP-1-FK506BP ameliorates corneal injury in Botulinum toxin A-induced dry eye mouse model. BMB Rep 46, 124-129 https://doi.org/10.5483/BMBRep.2013.46.2.272
  21. Wadia JS and Dowdy SF (2002) Protein transduction technology. Curr Opin Biotechnol 13, 52-56 https://doi.org/10.1016/S0958-1669(02)00284-7
  22. Kubo E, Fatma N, Akagi Y, Beier DR, Singh SP and Singh DP (2008) TAT-mediated PRDX6 protein transduction protects against eye lens epithelial cell death and delays lens opacity. Am J Physiol Cell Physiol 294, C842-C855 https://doi.org/10.1152/ajpcell.00540.2007
  23. Ahn EH, Kim DW, Kang HW et al (2010) Transduced PEP-1-ribosomal protein S3 (rpS3) ameliorates 12-O-tetradecanoyl phorbol-13-acetate-induced inflammation in mice. Toxicol 276, 192-197 https://doi.org/10.1016/j.tox.2010.08.004
  24. Dietz GP (2010) Cell-penetrating peptide technology to deliver chaperones and associated factors in diseases and basic research. Curr Pharm Biotechol 11, 167-174 https://doi.org/10.2174/138920110790909731
  25. Kwon SW, Sohn EJ, Kim DW et al (2011) Anti-inflammatory effect of transduced PEP-1-heme oxygenase-1 in Raw 264.7 cells and a mouse edema model. Biochem Biophys Res Commun 411, 354-359 https://doi.org/10.1016/j.bbrc.2011.06.147
  26. Shibagaki N, Okamoto T, Mitsui H, Inozume T, Kanzaki M and Shimada S (2011) Novel immunotherapeutic approaches to skin cancer treatments using protein transduction technology. J Dermatol Sci 61, 153-161 https://doi.org/10.1016/j.jdermsci.2010.12.003
  27. Ahn EH, Kim DW, Shin MJ et al (2013) PEP-1-ribosomal protein S3 protects dopaminergic neurons in an MPTP-induced Parkinson’s disease mouse model. Free Radic Biol Med 55, 36-45 https://doi.org/10.1016/j.freeradbiomed.2012.11.008
  28. An JJ, Eum WS, Kwon HS et al (2013) Protective effects of skin permeable epidermal and fibroblast growth factor against ultraviolet-induced skin damage and human skin wrinkles. J Cosmet Dermatol 12, 287-295 https://doi.org/10.1111/jocd.12067
  29. Liu J, Hou J, Xia ZY et al (2013) Recombinant PTD-Cu/Zn SOD attenuates hypoxia-reoxygenation injury in cardiomyocytes. Free Radic Res 47, 386-393 https://doi.org/10.3109/10715762.2013.780286
  30. Zhou G, Shan P, Hu X, Zheng X and Zhou S (2013) Neuroprotective effect of TAT PTD-Ngb fusion protein on primary cortical neurons against hypoxia-induced apoptosis. Neurol Sci 34, 1771-1778 https://doi.org/10.1007/s10072-013-1333-9
  31. Liu L, Yu R, Shi Y et al (2014) Transduced protein transduction domain linked HSP27 protected LECs against UVB radiation-induced damage. Exp Eye Res 120, 36-42 https://doi.org/10.1016/j.exer.2013.12.016
  32. Shin MJ, Kim DW, Lee YP et al (2014) Tat-glyoxalase protein inhibits against ischemic neuronal cell damage and ameliorates ischemic injury. Free Radic Biol Med 67, 195-210 https://doi.org/10.1016/j.freeradbiomed.2013.10.815
  33. Zhang Z, Huang L, Wu Q et al (2014) A recombinant trans-membrane protein hMn-SOD-R9 inhibits the proliferation of cervical cancer cells in vitro. Mol Cell Biochem 385, 79-86 https://doi.org/10.1007/s11010-013-1816-2
  34. Sommer HJ, Johnen J, Schongen P and Stolze HH (1994) Adaptation of the tear film to work in air-conditioned rooms (office-eye syndrome). Ger J Ophthalmol 3, 406-408
  35. Backman H and Haghighat F (1999) Indoor-air quality and ocular discomfort. J Am Optom Assoc 70, 309-316
  36. Nakamura S, Okada S, Umeda Y and Saito F (2004) Development of a rabbit model of tear film instability and evaluation of viscosity of artificial tear preparations. Cornea 23, 390-397 https://doi.org/10.1097/00003226-200405000-00015
  37. Koh S, Watanabe H, Hosohata J et al (2003) Diagnosing dry eye using a blue-free barrier filter. Am J Ophthalmol 136, 513-519 https://doi.org/10.1016/S0002-9394(03)00317-9
  38. Steinfeld A, Lux A, Maier S, Suverkrup R and Diestehorst M (2004) Bioavailability of fluorescein from a new drug delivery system in human eyes. Br J Ophthalmol 88, 48-53 https://doi.org/10.1136/bjo.88.1.48
  39. Nakamura S, Shibuya M, Saito Y et al (2003) Protective effect of D-beta-hydroxybutyrate on corneal epithelia in dry eye conditions through suppression of apoptosis. Invest Ophthalmol Vis Sci 44, 4682-4688 https://doi.org/10.1167/iovs.03-0198
  40. Higuchi A, Ueno R, Shimmura S, Suematsu M, Dogru M and Tsubota K (2007) Albumin rescues ocular epithelial cells from cell death in dry eye. Curr Eye Res 32, 83-88 https://doi.org/10.1080/02713680601147690
  41. Bradford M (1976) A rapid and sensitive method for the quantitation of microgram quantities utilizing the principle of protein-dye binding. Anal Biochem 72, 248-254 https://doi.org/10.1016/0003-2697(76)90527-3
  42. Cole KK and Perez-Polo JR (2002) Poly (ADP-ribose) polymerase inhibition prevents both apoptotic-like delayed neuronal death and necrosis after H2O2 injury. J Neurochem 82, 19-29 https://doi.org/10.1046/j.1471-4159.2002.00935.x
  43. Koo TB, Han MS, Tadashi Y, Seong WJ and Choi JY (2013) Differential expression of the metastasis suppressor KAI1 in decidual cells and trophoblast giant cells at the feto-maternal interface. BMB Rep 46, 507-512 https://doi.org/10.5483/BMBRep.2013.46.10.223

피인용 문헌

  1. Tat-NOL3 protects against hippocampal neuronal cell death induced by oxidative stress through the regulation of apoptotic pathways vol.38, pp.1, 2016, https://doi.org/10.3892/ijmm.2016.2596
  2. Tat-PRAS40 prevent hippocampal HT-22 cell death and oxidative stress induced animal brain ischemic insults vol.97, 2016, https://doi.org/10.1016/j.freeradbiomed.2016.06.009
  3. The efficacy of chimeric vaccines constructed with PEP-1 and Ii-Key linking to a hybrid epitope from heterologous viruses vol.43, pp.5, 2015, https://doi.org/10.1016/j.biologicals.2015.06.005
  4. Tat-HSP22 inhibits oxidative stress-induced hippocampal neuronal cell death by regulation of the mitochondrial pathway vol.10, pp.1, 2017, https://doi.org/10.1186/s13041-016-0281-8