Korean Journal of Environmental Agriculture (한국환경농학회지)

The Korean Society of Environmental Agriculture (한국환경농학회)
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Synthesis of Hapten for Indirect Competitive Immunoassay for Measuring 3,5,6-trichloro-2-pyridinol

  • Kim, Areumnuri (Chemical Safety Division, National Institute of Agricultural Sciences) ;
  • Kim, Joong-Young (BIONAST Co., Ltd.) ;
  • Jeong, Sang-Hee (Department of Applied Biotoxicology, Hoseo University) ;
  • Cho, Myung-Haing (College of Veterinary Medicine, Seoul National University) ;
  • Park, Kyung-Hun (Chemical Safety Division, National Institute of Agricultural Sciences) ;
  • Cho, Namjun (Chemical Safety Division, National Institute of Agricultural Sciences) ;
  • Paik, Min Kyoung (Chemical Safety Division, National Institute of Agricultural Sciences)
  • Received : 2015.09.10
  • Accepted : 2015.10.24
  • Published : 2015.12.31
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Abstract

BACKGROUND: In this study, we have attempted to identify a urinary biomarker to assess chlorpyrifos exposure in farmers. The major metabolite and the excretion pathway of chlorpyrifos is 3,5,6-trichloro-2-pyridinol (TCP) in urine. Herein, we describe an adequate synthetic method for TCP hapten for measuring urinary TCP of farmers. METHODS AND RESULTS: First, TCP was prepared by spacer attachment through hydrolysis of thiophosphate ester from chlorpyrifos. After reaction with benzyl bromide, the TCP was transformed into 2,3,5-trichloro-6-benzyloxypyridine. Next, the chlorine in the 2 nd position of the pyridyl ring was substituted into 3-mercaptopropanoic acid spacer arm. Finally, the phenyl group attached to the 6 th position in pyridyl ring was removed for producing the targeted product, 3-(3,5-Dichloro-6-hydroxy-2-pyridyl) thiopropanoic acid. CONCLUSION: Henceforth, this TCP hapten would be used in developing immunoassay studies for the detection and quantitation of urinary TCP of farmers.

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References

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