Journal of Dairy Science and Biotechnology

Korean Society of Dairy Science and Biotechnology (한국낙농식품응용생물학회)
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Rapid Detection of Viable Cronobacter sakazakii using Propidium Monoazide (PMA) in Combination with Real-Time qPCR

Propidium Monoazide(PMA)와 Real-Time qPCR을 이용한 살아있는 Cronobacter sakazakii의 신속검출

  • Hwang, Dae-Geun (KU Center for Food Safety and College of Veterinary Medicine, Konkuk University) ;
  • Chon, Jung-Whan (KU Center for Food Safety and College of Veterinary Medicine, Konkuk University) ;
  • Kim, Hyun-Sook (Dept. of Food & Nutrition, College of Human Ecology, Hanyang University) ;
  • Kim, Hong-Seok (KU Center for Food Safety and College of Veterinary Medicine, Konkuk University) ;
  • Kim, Dong-Hyeon (KU Center for Food Safety and College of Veterinary Medicine, Konkuk University) ;
  • Song, Kwang-Young (KU Center for Food Safety and College of Veterinary Medicine, Konkuk University) ;
  • Yim, Jin-Hyuk (KU Center for Food Safety and College of Veterinary Medicine, Konkuk University) ;
  • Kim, Young-Ji (KU Center for Food Safety and College of Veterinary Medicine, Konkuk University) ;
  • Kang, Il-Byung (KU Center for Food Safety and College of Veterinary Medicine, Konkuk University) ;
  • Seo, Kun-Ho (KU Center for Food Safety and College of Veterinary Medicine, Konkuk University)
  • 황대근 (건국대학교 수의과대학 및 KU 식품안전연구소) ;
  • 천정환 (건국대학교 수의과대학 및 KU 식품안전연구소) ;
  • 김현숙 (한양대학교 생활과학대학 식품영양학과) ;
  • 김홍석 (건국대학교 수의과대학 및 KU 식품안전연구소) ;
  • 김동현 (건국대학교 수의과대학 및 KU 식품안전연구소) ;
  • 송광영 (건국대학교 수의과대학 및 KU 식품안전연구소) ;
  • 임진혁 (건국대학교 수의과대학 및 KU 식품안전연구소) ;
  • 김영지 (건국대학교 수의과대학 및 KU 식품안전연구소) ;
  • 강일병 (건국대학교 수의과대학 및 KU 식품안전연구소) ;
  • 서건호 (건국대학교 수의과대학 및 KU 식품안전연구소)
  • Received : 2015.08.03
  • Accepted : 2015.09.15
  • Published : 2015.09.30
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Abstract

While various foodborne pathogenic bacteria can be detected more rapidly via polymerase chain reaction than via conventional plating methods, it is impossible to distinguish between viable and dead cells in DNA-based assays. Hence, propidium monoazide (PMA) treatment has been introduced to detect living cells. The purpose of this study is to evaluate the applicability of the PMA treatment and real-time qPCR method for the detection of Cronobacter sakazakii and to compare it to that of plate counting. Based on our positive results, we suggest the use of PMA treatment and real-time qPCR for the detection of viable Cronobacter sakazakii in various food sources and an update of the Korean Food Code.

Keywords

References

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