Journal of Dairy Science and Biotechnology

  • 제33권2호
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  • Pages.119-127
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  • 2015
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  • 2733-4554(pISSN)
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  • 2733-4562(eISSN)
한국낙농식품응용생물학회 (Korean Society of Dairy Science and Biotechnology)
권호 표지

낙농산업에 필요한 미생물 검사방법과 전망: 총설

Perspective on Rapid and Selective Method for Detecting Microbiology in Dairy Industry: A Review

  • 천정환 (건국대학교 수의과대학 및 KU 식품안전연구소) ;
  • 김현숙 (건국대학교 수의과대학 수의생리학전공) ;
  • 김홍석 (건국대학교 수의과대학 및 KU 식품안전연구소) ;
  • 김동현 (건국대학교 수의과대학 및 KU 식품안전연구소) ;
  • 송광영 (건국대학교 수의과대학 및 KU 식품안전연구소) ;
  • 임진혁 (건국대학교 수의과대학 및 KU 식품안전연구소) ;
  • 최다솜 (건국대학교 수의과대학 및 KU 식품안전연구소) ;
  • 임종수 (건국대학교 수의과대학 및 KU 식품안전연구소) ;
  • 정동관 (고신대학교 자연과학대학 식품영양학과) ;
  • 김수기 (건국대학교 동물생명과학대학 동물자원학과) ;
  • 서건호 (건국대학교 수의과대학 및 KU 식품안전연구소)
  • Chon, Jung-Whan (KU Center for Food Safety and College of Veterinary Medicine, Konkuk University) ;
  • Kim, Hyun-Sook (Dept. of Veterinary Physiology, College of Veterinary Medicine, Konkuk University) ;
  • Kim, Hong-Seok (KU Center for Food Safety and College of Veterinary Medicine, Konkuk University) ;
  • Kim, Dong-Hyeon (KU Center for Food Safety and College of Veterinary Medicine, Konkuk University) ;
  • Song, Kwang-Young (KU Center for Food Safety and College of Veterinary Medicine, Konkuk University) ;
  • Yim, Jin-Hyuk (KU Center for Food Safety and College of Veterinary Medicine, Konkuk University) ;
  • Choi, Dasom (KU Center for Food Safety and College of Veterinary Medicine, Konkuk University) ;
  • Lim, Jong-Soo (KU Center for Food Safety and College of Veterinary Medicine, Konkuk University) ;
  • Jeong, Dong-Gwan (Dept. of Food & Nutrition, College of Natural Science, Kosin University) ;
  • Kim, Soo-Ki (Dept. of Animal Science & Technology, College of Animal Bioscience & Technology, Konkuk University) ;
  • Seo, Kun-Ho (KU Center for Food Safety and College of Veterinary Medicine, Konkuk University)
  • 투고 : 2015.05.30
  • 심사 : 2015.06.15
  • 발행 : 2015.06.30
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초록

낙농 미생물학에서 사용하는 주요 분석 방법들에 대한 지속적인 개정이 요구되어지고 있는 것이 사실이다. 현재 이용 가능한 자료들을 바탕으로 균주의 생리 및 대사 특성을 더 깊이 이해할 수 있으며, LAB와 probiotic 생리적 상태를 측정할 수 있는 기술을 개발할 수 있다. 예를 들어, 고유 균주의 생화학 반응으로부터 얻은 생물학적 생리학적 지식을 바탕으로 발색형광배지(chromogenic media)의 개발이 가능하다. 이 기술은 오랫동안 사용되어온 한천 배지보다 사용법이 간단하며, 미생물을 제어할 수 있다. 유망한 유동세포계수법(flow cytometry, FC) 기술은 우유, 요구르트, 그 외 발효 유제품의 혼합 배양의 분석에 뛰어나다. 이 기술은 공정 과정 중에 또는 생산품의 유통 기한 관리에 사용될 수 있지만, 이 기술을 사용하기 위해서는 우유 단백질에 의해서 생성되는 인공 산물을 방지하기 위해 최적화 되어야 한다. 또한 유동세포계수법(flow cytometry, FC)은 정량 한계가 높기 때문에, 현재 사용하고 있는 탐침(probe)은 종 또는 속이 아닌 표적 세포 구성을 분석한다. 따라서 유동세포계수법(flow cytometry, FC)은 특정 물질의 계수보다는 생리적 상태를 측정에 더 적합한 기술이라고 볼 수 있다. Omics 자료를 통해 생리적 상태를 더 신속하게 측정할 수 있는 핵심적인 생체지표 확인이 가능해졌다. PCR은 이미 식품매개 병원균 및 부패균의 검출에 일상적으로 사용되고 있다. 이 기술은 더욱 나아가 발효균 및 probiotic 박테리아 검출에도 사용되어 핵산 추출, PCR 과정, 자료 해석을 표준화 시킬 수 있으며, 또한 다른 기술에 비해 PCR은 표적 발효 균주를 더 신속하게 선택하며, 공정 과정을 최적상태로 유지시킬 수 있을 것이다. LAB와 probiotic의 적응성 및 생리적 상태의 연구에 필요한 기준, 특허품, 상업화된 신속분석장치(kit)는 아직까지 개발되지 않았다. 본 총설 논문에서는 여러 가지 기술을 검토함으로써 이 모든 기술에는 2가지 주요한 요인이 있다. 첫 번째는 낙농업 산업의 특정 요구에 따른 방법의 평가이며, 두 번째는 공식적인 기준에 따른 검증이다. 사실상 식품매개 병원균의 특성 분석에 사용될 수 있는 표준법이 개발되고 검증되었지만, LAB, probiotic 등의 박테리아 분석에 있어서 표준화된 방법과 진단산업과의 연관성이 부족한 것이 사실이다. 낙농 업계에 의해서 수행되어진 품질관리의 양을 고려해 볼 때 이것은 매우 중요하게 인식되어야 할 부분이다.

To date, detection of microbial populations in dairy products has been performed using culture media, which is a time-consuming and laborious method. The recently developed chromogenic media could be more rapid and specific than classical culture media. However, the newly developed molecular-based technology can detect microbial populations with greater rapidity and sensitivity than the classical method involving culture media and chromogenic media. This molecular-based technology could provide various options for monitoring the characterization of different states of bacteria and cells. Thus, it could help upgrade the processing system of the dairy industry so as to maintain the safety and quality of dairy foods. Among the various newly developed molecular-based technologies, flow cytometry can potentially be used for monitoring microbiological populations in the dairy industry if official international standards are available for this purpose. When omics technology would have biomarker identification, it could be regarded as the rapid and sensitive analytical methods. Methods based on PCR, which has become a basic technique in microbiological research, can be developed and validated as alternative methods for quantification of dairy microorganisms. This review discusses methods for monitoring microbiological populations in dairy foods and the limitations of these studies, as well as the need for further research on such methods in the dairy industry.

키워드

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