생체재료학회지 (Biomaterials Research)

  • 제19권4호
  • /
  • Pages.203-209
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  • 2015
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  • 1226-4601(pISSN)
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  • 2055-7124(eISSN)
한국생체재료학회 (Korean Society for Biomaterials)
권호 표지
DOI QR코드

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Encapsulation of pancreatic islet with HMGB1 fragment for attenuating inflammation

  • Jo, Eun Hee (Department of Bioengineering, College of Engineering, Hanyang University) ;
  • Hwang, Yong Hwa (Department of Bioengineering, College of Engineering, Hanyang University) ;
  • Lee, Dong Yun (Department of Bioengineering, College of Engineering, Hanyang University)
  • 투고 : 2015.09.01
  • 심사 : 2015.10.01
  • 발행 : 2015.12.31
⟨ 이전 논문 다음 논문 ⟩

초록

Background: Pancreatic islet encapsulation is one way to address the disadvantages of islet transplantation. Not only does encapsulation involve bidirectional diffusion of nutrients, oxygen, and glucose, but also it protects the graft from the recipient's immune reaction. The high mobility group box 1 (HMGB1), one of higher expression proteins in islet, can be secreted from transplanted islets and induce the inflammation. Therefore, the regulation of HMGB1-mediated inflammation is very important for successful islet transplantation. In this study, we used the HMGB1 A box, an antagonist of HMGB1 receptor in the immune cells, in the encapsulation of isolated islets as a new strategy. Result: For co-encapsulation of HMGB1 A box protein with islets, we evaluated the distribution of alginate bead diameter. The average diameter of empty alginate bead was similar to that of alginate bead with islets. When different concentrations of HMGB1 A box protein was co-encapsulated with islets, it did not affect the viability and insulin secretion function of the islets. When the alginate beads with islets plus HMGB1 A box protein were cultured with macrophage, the amount of $TNF-{\alpha}$ secreted from the macrophages was significantly attenuated when compared to cultivation of unencapsulated islets or encapsulated islets. When the alginate beads with islets plus HMGB1 A box protein were intraperitoneally xenotransplanted into the diabetic mice, the survival rate of the islets was strongly improved with 2-fold. Conclusion: Collectively, these results suggested that the encapsulation of HMGB1 A box protein might offer a protective effect in islet transplantation.

키워드

과제정보

연구 과제 주관 기관 : National Research Foundation of Korea (NRF)

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피인용 문헌

  1. The Role of HMGB1 in the Pathogenesis of Type 2 Diabetes vol.2016, pp.None, 2015, https://doi.org/10.1155/2016/2543268
  2. Islet‐derived damage‐associated molecular pattern molecule contributes to immune responses following microencapsulated neonatal porcine islet xenotransplantation in mice vol.23, pp.5, 2015, https://doi.org/10.1111/xen.12253
  3. Vitexin alleviates lipopolysaccharide-induced islet cell injury by inhibiting HMGB1 release vol.15, pp.3, 2015, https://doi.org/10.3892/mmr.2017.6114
  4. The intraperitoneal space is more favorable than the subcutaneous one for transplanting alginate fiber containing iPS-derived islet-like cells vol.11, pp.None, 2015, https://doi.org/10.1016/j.reth.2019.05.003
  5. The effect of preexisting HMGB1 within fetal bovine serum on murine pancreatic beta cell biology vol.12, pp.1, 2015, https://doi.org/10.1080/19382014.2019.1696128