Biomaterials Research (생체재료학회지)

Korean Society for Biomaterials (한국생체재료학회)
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Electron beam effect on biomaterials I: focusing on bone graft materials

  • Kim, Soung Min (Department of Oral and Maxillofacial Surgery, Dental Research Institute, School of Dentistry, Seoul National University) ;
  • Fan, Huan (Department of Oral and Maxillofacial Surgery, Dental Research Institute, School of Dentistry, Seoul National University) ;
  • Cho, Yun Ju (Department of Oral and Maxillofacial Surgery, Dental Research Institute, School of Dentistry, Seoul National University) ;
  • Eo, Mi Young (Department of Oral and Maxillofacial Surgery, Dental Research Institute, School of Dentistry, Seoul National University) ;
  • Park, Ji Hyun (Quantum Optics Research Division, Korea Atomic Energy Research Institute) ;
  • Kim, Byung Nam (Radiation Instrumentation Research Division, Korea Atomic Energy Research Institute) ;
  • Lee, Byung Cheol (Quantum Optics Research Division, Korea Atomic Energy Research Institute) ;
  • Lee, Suk Keun (Department of Oral Pathology, College of Dentistry, Gangneung-Wonju National University)
  • Received : 2015.01.13
  • Accepted : 2015.03.30
  • Published : 2015.06.30
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Abstract

Background: To develop biocompatible bony regeneration materials, allogenic, xenogenic and synthetic bones have been irradiated by an electron beam to change the basic structures of their inorganic materials. The optimal electron beam energy and individual dose have not been established for maximizing the bony regeneration capacity in electron beam irradiated bone. Results: Commercial products consisting of four allogenic bones, six xenogenic bones, and six synthetic bones were used in this study. We used 1.0-MeV and 2.0 MeV linear accelerators (power: 100 KW, pressure; 115 kPa, temperature; -30 to $120^{\circ}C$, sensor sensitivity: 0.1-1.2 mV/kPa, generating power sensitivity: 44.75 mV/kPa, supply voltage: 50.25 V), and a microtrone with different individual irradiation doses such as 60 kGy and 120 kGy. Additional in vitro analyses were performed by elementary analysis using field emission scanning electron microscopy (FE-SEM), scanning electron microscopy (SEM), X-ray diffraction (XRD) and confocal laser scanning microscopy (CLSM). In vivo clinical, radiographic, and micro-computed tomography (Micro-CT) with bone marrow density (BMD) analysis was performed in 8- and 16-week-old Spraque-Dawley rats with calvarial defect grafts. Conclusions: Electron beam irradiation of bony substitutes has four main effects: the cross-linking of biphasic calcium phosphate bony apatite, chain-scissioning, the induction of rheological changes, and microbiological sterilization. These novel results and conclusions are the effects of electron beam irradiation.

Keywords

Acknowledgement

Supported by : National Research Foundation of Korea(NRF)

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