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배암차즈기 차 제조와 항산화 활성 및 품질특성

Antioxidative Activities and Qualitative Characteristics of Substitute Tea using Salvia plebeia R. Br.

  • 정지숙 (구례야생화연구소) ;
  • 김용주 (구례야생화연구소) ;
  • 안은경 (경기과학기술진흥원 천연물신약연구소) ;
  • 신주영 (경기과학기술진흥원 천연물신약연구소) ;
  • 고근배 (구례군농업기술센터 자원연구개발과) ;
  • 손병길 (구례군농업기술센터 자원연구개발과)
  • Jeong, Ji-Suk (Dept. of Wild Flower Institute of Resources Gurye-gun Agricultural Center) ;
  • Kim, Yong-Joo (Dept. of Wild Flower Institute of Resources Gurye-gun Agricultural Center) ;
  • Ahn, Eun-Kyung (Natural Products Research Institute, Gyeonggi Institute of Science & Technology Promotion) ;
  • Shin, Ju-Young (Natural Products Research Institute, Gyeonggi Institute of Science & Technology Promotion) ;
  • Go, Geun-Bae (Dept. of Research and Development of Resources Gurye-gun Agricultural Center) ;
  • Son, Byeong-Gil (Dept. of Research and Development of Resources Gurye-gun Agricultural Center)
  • 투고 : 2014.11.29
  • 심사 : 2015.01.27
  • 발행 : 2015.02.28

초록

항염 및 항산화 활성이 우수한 배암차즈기(SPR) 잎을 활용하여 배암차즈기 차(SPRT)를 제조하였다. SPR의 총 폴리페놀 및 총 플라보노이드 함량은 각각 $71.8{\pm}4.74GAE\;mg/g$$38.7{\pm}2.95QUE\;mg/g$으로 측정되었다. DPPH에 따른 수소공여능은 $16.9{\mu}g/mL$$RC_{50}$을 나타내었으며, 이때, 양성대조구인 ascorbic acid는 $6.1{\mu}g/mL$$RC_{50}$을 나타내었다. 배암차즈기는 세포 독성을 나타내지 않는 법 위에서 물 추출물의 $IC_{50}$$100{\mu}g/mL$ 이상이었으며, $25{\sim}100{\mu}g/mL$의 범위에서 NO 생성이 LPS 처리구에 비해 약 30% 정도 저해되었다. SPRT는 중잎과 대잎을 사용하여 자체수분을 활용하여 단시간 덖음하여 총 120분 가향한 차가 가장 좋은 평가를 받았다. 향은 맛과 전반적인 기호도에 양의관계로 영향을 주었다. SPRT는 덖음 및 가향 작업에 따른 열처리에도 항산화 수치가 통계적으로 유의적인 차이가 없어 항산화능이 유지되었다. 티백량은 1.0 g이 적당한 것으로 평가되었다. 따라서 본 실험의 결과를 바탕으로 배암차즈기가 항산화 등 기능성을 강조한 음료 또는 식품 소재로 활용될 수 있을 것이다.

The substitute tea was manufactured from the leaves of Salvia plebeia R. Br. (SPR) that have anti-inflammatory and antioxidative activities. Total polyphenols and flavonoids in SPR were $71.8{\pm}4.74GAE\;mg/g$ and $38.7{\pm}2.95QUE\;mg/g$, respectively. DPPH radical scavenging activity of SPR water extract had an $RC_{50}$ of $16.9{\mu}g/mL$. SPR inhibited lipopolysaccharide (LPS)-induced nitric oxide (NO) production with an $IC_{50}$ of > $100{\mu}g/mL$. Medium and large leaves were used in the SPR substitute tea (SPRT). The water content was dissipated on roasting within approximately 10 minutes, and the tea obtained from fan-firing for a total 120 minutes was evaluated as most superior. Flavor was positively correlated with the taste and general preference. SPRT maintained its anti-oxidative activity without significant changes on roasting or fan-firing even after the heat treatment. The appropriate weight of a tea bag for one serving was 1.0 g. The study results indicated that SPR is a potential resource for the development of food or ingredients with anti-inflammatory properties.

키워드

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