펄프종이기술 (Journal of Korea Technical Association of The Pulp and Paper Industry)

한국펄프종이공학회 (Korea Technical Association of the Pulp and Paper Industry)
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MicroFibrillated Cellulose(MFC) 제조를 위한 전처리 효소의 활성 및 고해 특성

Enzyme Activity and Beating Properties for Preparation of MicroFibrillated Cellulose(MFC)

  • 김강재 (경북대학교 농업생명과학대학 임산공학과) ;
  • 정진동 (경북대학교 농업생명과학대학 임산공학과) ;
  • 정수은 (경북대학교 농업생명과학대학 임산공학과) ;
  • 안은별 (경북대학교 농업생명과학대학 임산공학과) ;
  • 엄태진 (경북대학교 농업생명과학대학 임산공학과)
  • Kim, Kang-Jae (Dept. of Wood Science and Technology, College of Agriculture and Life Science, Kyungpook National University) ;
  • Jung, Jin-Dong (Dept. of Wood Science and Technology, College of Agriculture and Life Science, Kyungpook National University) ;
  • Jung, Soo-Eune (Dept. of Wood Science and Technology, College of Agriculture and Life Science, Kyungpook National University) ;
  • Ahn, Eun-Byeoul (Dept. of Wood Science and Technology, College of Agriculture and Life Science, Kyungpook National University) ;
  • Eom, Tae-Jin (Dept. of Wood Science and Technology, College of Agriculture and Life Science, Kyungpook National University)
  • 투고 : 2015.01.14
  • 심사 : 2015.02.23
  • 발행 : 2015.02.28
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초록

In this study, we evaluated optimum condition of enzyme with pH and temperature for preparation of microfibillated cellulose(MFC). Well-known endo-glucanase, three enzymes were used and CMC was used for substrate. Enzyme activity was evaluated using DNS method and absorbance with UV/VIS spectrophotometer. The enzyme shown the greatest activity was reacted with pulps at optimum condition for 1 hour and treated pulps beated until 100 mL CSF. Enzyme B and Enzyme L was the higher enzyme activity below 0.1% concentration and Enzyme N was the lowest enzyme activity. At various pH and temperature conditions, enzyme activity of Enzyme B was higher than the others at the same concentration. Especially enzyme activity at $50^{\circ}C$ of Enzyme B was almost not changed over pH 6.0. Optimum condition of three enzyme was pH 6 or pH 7 and $50^{\circ}C$ or $60^{\circ}C$. Also beating efficiency of enzyme treated pulps with Enzyme B is 55.6%.

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참고문헌

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