Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
권호 표지
DOI QR코드

DOI QR Code

A Specific Biomarker for The Diagnosis of Equine Laminitis

말의 제엽염 진단을 위한 특이적 생물지표

  • Lee, Seungwoo (College of Veterinary Medicine, Seoul National University) ;
  • Lee, Seung Heon (College of Veterinary Medicine, Seoul National University) ;
  • Kim, Myung-Chul (College of Veterinary Medicine, Seoul National University) ;
  • Kim, Yong-Baek (College of Veterinary Medicine, Seoul National University) ;
  • Ryu, Doug-Young (College of Veterinary Medicine, Seoul National University)
  • Accepted : 2014.01.25
  • Published : 2015.02.28
Download PDF
⟨ Previous Next ⟩

Abstract

There is no reliable indicator available for the diagnosis of horse laminitis, although the disease is common and costly. This study was performed to develop a specific diagnostic biomarker for laminitis. We have identified 33 differentially expressed proteins in plasma of a horse suffering laminitis that is experimentally induced by an overdose of oligofructose, in comparison with normal horse plasma. Among the proteins, myosin-9 mRNA was found in RNA sequencing analysis to be expressed specifically in laminitis tissues compared to other horse tissues. It is thus suggested that expression of plasma myosin-9 may be used for the diagnosis of equine laminitis.

Keywords

References

  1. 권미영, 류덕영, 전형선, 김준규, 최봉희, 이승우, 김용백. 올리고당 투 여를 통한 급성 제엽염의 실험적 유발. 한국임상수의학회지 2013; 30: 75-79.
  2. Bailey SR, Baillon ML, Rycroft AN, Harris PA, Elliott J. Identification of equine cecal bacteria producing amines in an in vitro model of carbohydrate overload. Appl Environ Microbiol 2003; 69: 2087-2093. https://doi.org/10.1128/AEM.69.4.2087-2093.2003
  3. Bailey SR, Katz LM, Berhane Y, Samuels T, De Brauvere N, Marr CM, Elliott J. Seasonal changes in plasma concentrations of cecum-derived amines in clinically normal ponies and ponies predisposed to laminitis. Am J Vet Res 2003; 64: 1132-1138. https://doi.org/10.2460/ajvr.2003.64.1132
  4. van Eps AW, Pollitt CC. Equine laminitis induced with ligofructose. Equine Vet J. 2006; 38: 203-208.
  5. Fontaine GL, Belknap JK, Allen D, Moore JN, Kroll DL. Expression of interleukin-1beta in the digital laminae of horses in the prodromal stage of experimentally induced laminitis. Am J Vet Res 2001; 62: 714-720. https://doi.org/10.2460/ajvr.2001.62.714
  6. Garner HE, Coffman JR, Hahn AW, Hutcheson DP, Tumbleson ME. Equine laminitis of alimentary origin: an experimental model. Am J vet Res 1975; 36: 441-444.
  7. Johnson PJ, Kreeger JM, Keeler M, Ganjam VK, Messer NT. Serum markers of lamellar basement membrane degradation and lamellar histopathological changes in horses affected with laminitis. Equine Vet J 2000; 32: 462-468.
  8. Kalck KA, Frank N, Elliott SB, Boston RC. Effects of lowdose oligofructose treatment administered via nasogastric intubation on induction of laminitis and associated alterations in glucose and insulin dynamics in horses. Am J Vet Res 2009; 70: 624-632. https://doi.org/10.2460/ajvr.70.5.624
  9. Kim S, Jho EH. The protein stability of Axin, a negative regulator of Wnt signaling, is regulated by Smad ubiquitination regulatory factor 2 (Smurf2). J Biol Chem 2010; 285: 36420-36426. https://doi.org/10.1074/jbc.M110.137471
  10. Longland AC, Byrd BM. Pasture nonstructural carbohydrates and equine laminitis. J Nutr 2006; 136: 2099S-2102S. https://doi.org/10.1093/jn/136.7.2099S
  11. Metzker ML. Sequencing technologies - the next generation. Nat Rev Genet 2010; 11: 31-46. https://doi.org/10.1038/nrg2626
  12. Baldwin MA. Protein Identification by Mass Spectrometry. Mol Cell Proteomics 2004; 3: 1-9. https://doi.org/10.1074/mcp.R300012-MCP200
  13. Pollitt CC. Laminitis in perspective. In: Equine laminitis, current concept. Canberra: Rural Industries Research and Development Corporation. 2008; 19-68.
  14. Steelman SM, Chowdhary BP. Plasma proteomics shows an elevation of the anti-inflammatory protein APOA-IV in chronic equine laminitis. BMC Vet Res 2012; 8: 179-188. https://doi.org/10.1186/1746-6148-8-179
  15. Trapnell C, Roberts A, Goff L, Pertea G, Kim D, Kelley DR, Pimentel H, Salzberg SL, Rinn JL, Pachter L. Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and Cufflinks. Nat Protoc 2012; 7: 562-578. https://doi.org/10.1038/nprot.2012.016
  16. Twine NA, Janitz K, Wilkins MR, Janitz M. Whole transcriptome sequencing reveals gene expression and splicing differences in brain regions affected by Alzheimer's disease. PLoS One 2011; 6: e16266-e16278. https://doi.org/10.1371/journal.pone.0016266
  17. Vijaya Bhaskar V, Middha A. Liquid chromatography/tandem mass spectrometry method for quantitation of cremophor el and its applications. Int J Anal Chem 2013; 2013: 135613-135623.