Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
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Evaluation of Extended Boar Semen after Glass Wool Filtration

Glass Wool Filtration 후 돼지정액의 평가

  • Kang, Tae-Young (College of Veterinary Medicine, Jeju National University)
  • 강태영 (제주대학교 수의과대학)
  • Accepted : 2015.01.28
  • Published : 2015.02.28
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Abstract

The purpose of this study was to select high-quality boar semen after the glass wool filtration of extended boar semen. After collecting boar semen, its concentration, morphology, viability, and motility were examined according the glass wool's height and time. After glass wool filtration, the sperm concentration decreased, but the proportion of normal sperms and the sperm viability increased. Nevertheless, the sperm motility showed no changes. The above results showed that the glass wool filtration of boar semen is a method of obtaining sperms with relatively low abnormal rates and high viabilities.

돼지의 희석정액을 glass wool filtration 한 후 양질의 정자를 선별하고자 실시하였다. 돼지의 정액을 채취한 후 glass wool 높이와 시간에 따른 정자의 농도, 기형율, 생존율, 그리고 운동성을 조사하였다. Glass wool 여과 후 정자의 농도는 줄었으나, 정상 정자비율과 생존율은 상승하였고, 운동성의 변화는 나타나지 않았다. 이상의 결과들을 통해 돼지 정자의 glass wool 여과는 상대적으로 낮은 기형율과 높은 생존율을 가진 정자를 얻을 수 있는 방법임을 알 수 있었다.

Keywords

References

  1. Ahmad Z, Anzar M, Shahab M, Ahmad N, Andrabi SMH. Sephadex and sephadex ion exchange filtration improves the quality and freezability of low grade buffalo semen ejaculates. Theriogenology 203; 59: 1189-1202. https://doi.org/10.1016/S0093-691X(02)01159-7
  2. Anzar M, Graham EF. Filtration of bovine semen. II. Factors affecting the recovery rate of spermatozoa. Anim Reprod Sci 1993; 31: 197-204. https://doi.org/10.1016/0378-4320(93)90004-B
  3. Anzar M, Graham EF. Effect of filtration on post-thaw quality of bull semen. Theriogenology 1995; 43: 439-449. https://doi.org/10.1016/0093-691X(94)00037-U
  4. Bussalleu E, Pinart E, Rivera MM, Briz M, Sancho S, Yeste M, Faberga A, Rigau T, Rodriguez JE, Bonet S. Effects of matrix filtration of low-quality boar semen doses on sperm quality. Reprod Domest Anim 2009; 44: 499-503. https://doi.org/10.1111/j.1439-0531.2008.01221.x
  5. Bussalleu E, Pinart E, Yeste M, Briz M, Sancho S, Garcia-Gil N, Badia E, Bassols J, Pruneda A, Casas I, Bonet S. Development of a protocol for multipl staining with fluorochromes to assess the fuctional status of boar spermatozoa. Microsc Res Tech 2005; 68:277-283. https://doi.org/10.1002/jemt.20246
  6. Cisale HO, Fischman ML, Blasi CD, Femandez HA, Gledhill BL. Enrichment of high quality spermatozoa in bovine semen: relative effectiveness of three filtration matrixes. Andrologia 2001; 33: 143-150. https://doi.org/10.1046/j.1439-0272.2001.00413.x
  7. Engel S, Weber H, Petzoldt R, Seidle B, Wiehe W, Slperl J. An improved method of sperm selection by glass wool filtration. Andrologia 2001; 33: 223-230. https://doi.org/10.1046/j.1439-0272.2001.00434.x
  8. Gerrits R, Lunney J. Johnson A. Pursel V. Kraeling R, Rohrer G, Dobrinsky J. Perspectives for artificial insemination and genomics to improve global swine populations. Theriogenology 2005; 63: 283-299. https://doi.org/10.1016/j.theriogenology.2004.09.013
  9. Gorus FK, Piper DG. A rapid method for the fractionation of human spermatozoa according to their progressive motility. Fertil Steril 1981; 35: 662-665. https://doi.org/10.1016/S0015-0282(16)45561-5
  10. Graham EF, Graham JK. The effect of whole ejaculate filtration on morphology and the fertility of bovine semen. J Dairy Sci 1990; 73: 91-97. https://doi.org/10.3168/jds.S0022-0302(90)78650-X
  11. Januskauskas A, Lukoseviciute K, Nagy, Johannisson A, Rodriguez-Martinez H. Assessment of the efficacy of Sephadex G-15 filtration of bovine spermatozoa for cryopreservation. Theriogenology 2005; 63: 160-178. https://doi.org/10.1016/j.theriogenology.2004.04.002
  12. Johnson LA, Aalbers JG, Willems CM, Rademaker JH, Rexroad CE Jr. Use of boar spermatozoa for artificial insemination. III. Fecundity of boar spermatozoa stored in Beltsville liquid and Kiev extenders for three days at 18 C. J Anim Sci 1982; 54: 132-136. https://doi.org/10.2527/jas1982.541132x
  13. Johnson LA, Weitze KF, Fiser P, Maxwell WMC. Storage of boar semen. Anim Reprod Sci 2000; 62: 143-172. https://doi.org/10.1016/S0378-4320(00)00157-3
  14. Lopata A, Patullo MJ, Chang A, James B. A method for collection motile spermatozoa from human semen. Fertil Steril 1976; 27: 677-684. https://doi.org/10.1016/S0015-0282(16)41899-6
  15. Maki-Laurila M, Graham EF. Separation of dead and live spermatozoa in bovine semen. J Dairy Sci 1968; 51: 965.
  16. Mogas T, Rigau T, Piedrafita J, Bonet S, Rodriguez-Gil JE. Effect of column filtration upon the quality parameters of fresh dog semen. Theriogenology 1998; 50: 1171-1189. https://doi.org/10.1016/S0093-691X(98)00218-0
  17. Morrel JM. Update on semen technologies for animal breeding. Repro Domest Anim 2006; 41: 63-67.
  18. Paulson JD, Polakoski KL. A glass wool procedure for removing extraneous material from the human ejaculate. Fertil Steril 1977; 28: 178-181. https://doi.org/10.1016/S0015-0282(16)42379-4
  19. Roca J, Rod iguez-Martinez H, Vazquez JM, Bolarin A, Hernandez M, Saravia F, Wallgren M, Martinez EA. Strategies to improve the fertility of frozen-thawed boar semen for artificial insemination. Soc Reprod Fertil Suppl. 2006; 62: 261-275.
  20. Roldan ER. Signal transduction during mammalian sperm acrosomal exocytosis. In: Gamates: Development and Fuction. Rome: Serono Symposia. 1998: 219-228.
  21. Samper JC. Evaluation of Membrane Integrity of Preserved Stallion Spermatozoa. PhD Dissertation, University of Minnesota. 1990.
  22. Tsakmakidis I, Lymberopoulos A, Khalifa T. Relationship between sperm quality traits and field-fertility of porcine semen. J Vet Sci 2010; 11: 151-154. https://doi.org/10.4142/jvs.2010.11.2.151
  23. Waberski D, Petrunkina A, Topfer-Petersen E. Can external quality controlimprove pig AI efficiency? Theriogenology 2008; 70: 1346-1351. https://doi.org/10.1016/j.theriogenology.2008.06.006