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Occurrence of Rust on Peucedanum japonicum Caused by Puccinia jogashimensis in Korea

Puccinia jogashimensis에 의한 갯기름나물 녹병

  • Ko, Sug-Ju (Jeollanam-do Agricultural Research and Extension Services) ;
  • Kim, Hyo-Jeong (Jeollanam-do Agricultural Research and Extension Services) ;
  • Myung, Inn-Shik (Crop Protection Division, National Institute of Agricultural Science, Rural Development Administration) ;
  • Uhm, Mi-Jeong (Jeollabuk-do Agricultural Research and Extension Services) ;
  • Choi, In-Young (Jeollabuk-do Agricultural Research and Extension Services)
  • Received : 2015.09.22
  • Accepted : 2015.11.06
  • Published : 2015.12.31

Abstract

During July to November 2014, severe rust infection was consistently found on Peucedanum japonicum growing farm in Yeosu, Korea. The rust was observed mainly on lower leaf surfaces. Symptoms of typical plants included yellow-orange rust pustules were observed on the petiole and leaf surface with small yellowish to chlorotic lesions on the upper surface. No symptom was observed on flowers. Uredinia were occurred amphigenous on leaf surface, and occasionally caulicolous, scattered or loosely aggregate, rounded to oblong, 0.4 to 4 mm in diameter, covered by epidermis, then naked, surrounded by ruptured epidermis, pulverulent, and brown. Urediniospores were ovate-ellipsoid, ellipsoid or subglobose, light brown, 20 to $45{\times}15$ to $35{\mu}m$, walls 2 to $4{\mu}m$ thick. The resulting sequences were deposited in GenBank with accession No. KT778808, KT778809, and KT778810, respectively. Since this was the first accession of 28S sequence Puccinia jogashimensis, there was no exact match in GenBank nucleotide database. On the basis of the morphological characteristics and phylogenetic analyses of 28S rDNA, the fungus was identified as P. jogashimensis. To our knowledge, this is the first confirmed report on the occurrence of P. jogashimensis on P. japonicum in Korea.

2014년 7월 중순부터 11월까지 전라남도 여수시 금오도의 농가포장에 갯기름나물 녹병이 발생되었다. 녹병은 새로운 잎보다는 아래로 늘어진 오래된 잎에서 주로 발병되었는데, 병징은 잎 앞면과 뒷면, 그리고 잎자루에 주황색 모양의 여름포자퇴를 만들었으며, 그 안에는 대량의 포자를 가지고 있었다. 그러나 갯기름나물 꽃에는 녹병이 관찰되지 않았다. 녹병에 심하게 감염된 식물체는 여름포자퇴 주변에서 갈색형태의 반점을 보였으며, 잎이 황화되거나 고사되고 조기 노화를 가져왔다. 녹병균의 여름포자퇴는 잎 표면의 앞 뒷면과 잎자루에 발생하였으며, 개별적으로 형성되거나 때때로 군집하여 형성되기도 하였다. 둥근 원형에서 길쭉한 반구 형태로 표피로 덮여 있거나, 벗겨진 표피로 둘러 쌓여진 형태로 직경은 0.1-4 mm이며, 분말형태의 구릿빛 갈색을 띄고 있었다. 여름포자는 달걀모양에서 타원형으로 겉 표면에 가시 같은 돌기가 있는 밝은 갈색으로 $20-45{\times}15-35{\mu}m$이며, 벽의 두께는 $2-4{\mu}m$이었다. 28S rDNA의 염기서열을 NCBI에서 BLAST search한 결과 등록된 GenBank accession number는 없는 것으로 확인되었다. 따라서 녹병의 발생생태, 균학적 특징, 28S rDNA의 D1/D2의 염기서열 분석 등의 결과를 바탕으로 기존의 보고된 P. jogashimensis에 의한 갯기름나물 녹병을 다시 보완하고자 한다.

Keywords

References

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