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베리류 에탄올 추출물의 항산화 활성

Antioxidant Activities of Various Berries Ethanolic Extract

  • 이화 ((주)벤스랩 중앙연구소) ;
  • 정종문 (수원대학교 생명과학과)
  • Li, Hua (Research Center of Ben's Lab Co., Ltd.) ;
  • Jeong, Jong Moon (Department of Life Science, The University of Suwon)
  • 투고 : 2014.08.05
  • 심사 : 2015.01.05
  • 발행 : 2015.02.28

초록

Edible berries are rich in anthocyanins and phenolic acids, compounds that possess antioxidant, anti-inflammatory, and other biological activities. Antioxidant and anti-inflammatory activities of five berries including acaiberry (Euterpe oleracea Mart.), Aronia/black chokeberry (Aronia melanocarpa), blueberry (Vaccinium angustifolium), black currant (Ribes nigrum L.), and cranberry (Vaccinium macrocarpon) were assessed. The Aronia G (prepared by GreenField s.c.) exhibited the highest antioxidant activities as shown in total phenolic (138.81 mg CAE/g), flavonoid (3.68 mg QE/g), and anthocyanin (20.31 mg/g) contents compared to the other berries. It also showed the strongest scavenging activities such as DPPH (69.69 mg vitamin C/g) and ABTS radical scavenging activity ($757.79{\mu}mol$ trolox/g). Aronia G exhibited strong ferric reducing antioxidant power ($553.98{\mu}mol$ vitamin C/g), and oxygen radical absorbance capacity ($820.92{\mu}mol$ trolox/g). In addition, black currant and Aronia showed stronger inhibition of nitric oxide production in lipopolysaccharide-stimulated RAW 264.7 cell than the other berries. According to the above results, the Aronia and other edible berries have notably high level of antioxidant activities and they could be used as a potential source of natural antioxidants.

키워드

참고문헌

  1. Bakar MFA, Mohamed M, Rahmat Am and Fry J. (2009). Phytochemicals and antioxidant activity of different parts of bambangan(Mangifera pajang) and tarap(Artocarpus odoratissimus). Food Chemistry. 113:479-483. https://doi.org/10.1016/j.foodchem.2008.07.081
  2. Basu A, Rhone M and Lyons TJ. (2010). Berries: Emerging impact on cardiovascular health. Nutrition Reviews. 68:168-177. https://doi.org/10.1111/j.1753-4887.2010.00273.x
  3. Benvenuti S, Pellati F, Melegari M and Bertelli D. (2004). Polyphenols, anthocyanins, ascorbic acid, and radical scavenging activity of Rubus, Ribes, and Aronia. Journal of Food Science. 69:FCT164-FCT169.
  4. Byeon SE, Chung JY, Lee YG and Kim BH. (2008). In vitro and In vivo anti-inflammatory effects of taheebo, a water extract from the inner bark of Tabebuia avellanedae. Journal of Ethnopharmacology. 119:145-152. https://doi.org/10.1016/j.jep.2008.06.016
  5. Chung YC, Chen SJ, Hsu CK, Chang CT and Chou ST. (2005). Studies on the antioxidative activity of Graptopetalum paraguayense E. Walther. Food Chemistry. 91:419-424. https://doi.org/10.1016/j.foodchem.2004.06.022
  6. Choi KH, Nam HH and Choo BK. (2013). Effect of five Korean native Taraxacum on antioxidant activity and nitric oxide production inhibitory activity. Korean Journal of Medicinal Crop Science. 21:191-196. https://doi.org/10.7783/KJMCS.2013.21.3.191
  7. Garbacki N, Tits M, Angenot L and Damas J. (2004). Inhibitory effects of proanthocyanidins from Ribes nigrum leaves on carrageenin acute inflammatory reactions induced in rats. BioMed Central Phamacology. 4:1-9. https://doi.org/10.1186/1471-244X-4-1
  8. Gasiorowski K, Szyba K, Brokos B, Kolaczynska B, Jankowiak-Wlodarczyk M and Oszmianski. (1997). Antimutagenic activity of anthocyanins isolated from Aronia melanocarpa fruits. Cancer Letters. 119:37-46. https://doi.org/10.1016/S0304-3835(97)00248-6
  9. Grace MH, Ribnicky DM, Kuhn P, Poulev A, Logendra S, Yousef GG, Raskin I and Lila MA. (2009). Hypoglycemic activity of a novel anthocyanin-rich formulation from lowbush blueberry, Vaccinium angustifolium Aiton. Phytomedicine. 16:406-415. https://doi.org/10.1016/j.phymed.2009.02.018
  10. Gutfinger T. (1981). Polyphenols in olive oils. Journal of the American Oil Chemists' Society. 58:966-968. https://doi.org/10.1007/BF02659771
  11. Halliwell B and Gutteridge JMC. (2003). Free radicals in biology and medicine. Oxford University Press. Oxford, England. p.246-350.
  12. Huang WY, Majumder K and Wu J. (2010). Oxygen radical absorbance capacity of peptides from egg white protein ovotransferrin and their interaction with phytochemicals. Food Chemistry. 123:635-641. https://doi.org/10.1016/j.foodchem.2010.04.083
  13. Jeong CH, Jang CW, Lee KY, Kim IH and Shim KW. (2012). Chemical components and anti-oxidant activities of black currant. Korean Journal of Food Preservation. 19:263-270. https://doi.org/10.11002/kjfp.2012.19.2.263
  14. Kalt W, Forney CF, Martin A and Prior RL. (1999). Antioxidant capacity, vitamin C, phenolics, and anthocyanins after fresh storage of small fruits. Journal of Agricultural and Food Chemistry. 47:4638-4644. https://doi.org/10.1021/jf990266t
  15. Kay CD and Holub BJ. (2002). The effect of wild blueberry (Vaccinium angustifolium) consumption on postprandial serum antioxidant status in human subjects. British Journal of Nutrition. 88:389-397. https://doi.org/10.1079/BJN2002665
  16. Knox YM, Suzutani T, Yosida I and Azuma M. (2003). Anti-influenza virus activity of crude extract of Ribes nigrum L. Phytotherapy Research. 17:120-122. https://doi.org/10.1002/ptr.1053
  17. Kwak HY, Lee SJ, Lee DY, Jung L, Bae NH, Hong SY, Kim GW and Baek NI. (2008). Cytotoxic and anti-inflammatory activities of lipids from the Nuruk(Rhizopus oryzae KSD-815). Journal of the Korean Society for Applied Biological Chemistry. 51:142-147.
  18. Lala G, Malik M, Zhao CW, He J, Kwon Y, Giusti MM and Magnuson BA. (2006). Anthocyanin-rich extracts inhibits multiple biomarkers of colon cancer in rats. Nutrition and Cance. 54:84-93. https://doi.org/10.1207/s15327914nc5401_10
  19. Lee J. (2005). Determination of total monomeric anthocyanin pigment content of fruit juices, beverages, natural colorants, and wines by the pH differential method: collaborative study. Journal of AOAC International. 88:1269-1278.
  20. Lee SJ, Choi HR, Lee JC, Park HJ, Lee HK, Jeong JT and Lee TB. (2014). The anti-aging effects of various berries in the human skin keratinocyte(HeCaT) cells. Korean Journal of Food Science and Technology. 46:198-204. https://doi.org/10.9721/KJFST.2014.46.2.198
  21. Lichtenthaler R, Rodrigues RB, Maia JGS, Papagiannopoulos M, Fabricius H and Marx F. (2005). Total oxidant scavenging capacities of Euterpe oleracea Mart. (Acai) fruits. International Journal of Food Sciences and Nutrition. 56:53-64. https://doi.org/10.1080/09637480500082082
  22. Martineau LC, Couture A, Spoor D, Benhaddou-Andaloussi A, Harris C, Meddah B, Leduc C, Burt A, Vuong T, Le PM, Prentki M, Bennett SA, Arnason JT and Haddad PS. (2006). Anti-diabetic properties of the Canadian lowbush blueberry Vaccinium angustifolium Ait. Phytomedidine. 13:612-623. https://doi.org/10.1016/j.phymed.2006.08.005
  23. Matchett MD, MacKinnon SL, Sweeney MI, Gottschall-Pass KT and Hurta RAR. (2006). Inhibition of matrix metalloproteinase activity in DU145 human prostate cancer cells by flavonoids from lowbush blueberry(Vaccinium angustifolium): Possible roles for protein kinase C and mitogen-activated protein-kinase-mediated events. Journal of Nutritional Biochemistry. 17:117-125. https://doi.org/10.1016/j.jnutbio.2005.05.014
  24. McKay DL and Blumberg JB. (2007). Cranberries(Vaccinium macrocarpon) an cardiovascular disease risk factors. Nutrition Reviews. 65:490-502. https://doi.org/10.1301/nr.2007.nov.490-502
  25. Moreno MIN, Isla MI, Sampietro AR and Vattuone MA. (2000). Comparison of the free radical-scavenging activity of propolis from several regions of Argetina. Journal of Ethnopharmacology. 71:109-114. https://doi.org/10.1016/S0378-8741(99)00189-0
  26. Moyer RA, Hummer KE, Finn CE, Frei B and Wrolstad RE. (2002). Anthocyanins, phenolics, and antioxidant capacity in diverse small fruits: Vaccinium, Rubus, and Ribes. Journal of Agricultural and Food Chemistry. 50:519-525. https://doi.org/10.1021/jf011062r
  27. Niki E, Shimaski H and Mino M. (1994). Antioxidantism-Free radical and biological defence. Gakkai Syuppan Center. Tokyo, Japan. p.3-16.
  28. Ohgami K, Ilieva I, Shiratori K, Koyama Y, Jin XH, Yoshida K, Kase S, Kitaichi N, Suzuki Y, Tanaka T and Ohno S. (2005). Anti-inflammatory effects of aronia extract on rat endotoxin-induced uveitis. Investigative Ophthalmology & Visual Science. 46:275-281. https://doi.org/10.1167/iovs.04-0715
  29. Park HJ, Han ES, Park DK, Lee C and Lee KW. (2010). An extract of Phellinus linteus grown on germinated brown rice inhibits inflammation markers in RAW 264.7 macrophages by suppressing inflammatory cytokines, chemokines, and mediators and upregulating antioxidant activity. Journal of Medicinal Food. 13:1468-1477. https://doi.org/10.1089/jmf.2010.1131
  30. Pozo-Insfran DD, Percival SS and Talcott ST. (2006). Acai(Euterpe oleracea Mart.) polyphenolics in their glycoside and aglycone forms induce apoptosis of HL-60 leukemia cells. Journal of Agricultural and Food Chemistry. 54:1222-1229. https://doi.org/10.1021/jf052132n
  31. Prior RL, Cao G, Martin A, Sofic E, McEwen J, O' Brien C, Lischner N, Ehlenfeldt M, Kalt W, Krewer G and Mainand CM. (1998). Antioxidant capacity as influenced by total phenolic acid and anthocyanin content, maturity, and variety of Vaccinium species. Journal of Agricultural and Food Chemistry. 46:2686-2693. https://doi.org/10.1021/jf980145d
  32. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M and Rice-Evans C. (1999) Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine. 26:1231-1237. https://doi.org/10.1016/S0891-5849(98)00315-3
  33. Slimestad R, Torskangerpoll K, Nateland HS, Johannessen T and Giske NH. (2005). Flavonoids from black chokeberries, Aronia melanocarpa. Journal of Food Composition and Analysis. 18:61-68. https://doi.org/10.1016/j.jfca.2003.12.003
  34. Taruscio TG, Barney DL and Exon J. (2004). Content and profile of flavanoid and phenolic acid compounds in conjunction with the antioxidant capacity for a variety of northwest Vaccinium berries. Journal of Agricultural and Food Chemistry. 52:3169-3176. https://doi.org/10.1021/jf0307595
  35. Valcheva-Kuzmanova S, Borisova P, Galunska B, Krasnaliev I and Belcheva A. (2004). Hepatoprotective effect of the natural fruits juice from Aronia melanocarpa on carbon tetrachloride-induced acute liver damage in rats. Experimental and Toxicologic Pathology. 56:195-201. https://doi.org/10.1016/j.etp.2004.04.012
  36. Valcheva-Kuzmanova S, Kuzmanov K, Mihova V, Krasnaliev I, Borisova P and Belcheva A. (2007). Antihyperlipidemic effect of Aronia melanocarpa fruit juice in rats fed a high-cholesterol diet. Plant Foods for Human Nutrition. 62:19-24. https://doi.org/10.1007/s11130-006-0036-2
  37. Wang SY and Lin H. (2000). Antioxidant activity in fruits and leaves of blackberry, rasberry, and strawberry varies with cultivar and developmental stage. Journal of Agricultural and Food Chemistry. 48:140-148. https://doi.org/10.1021/jf9908345
  38. Wang SY and Stretch AW. (2001). Antioxidant capacity in cranberry is influenced by cultivar and storage temperature. Journal of Agricultural and Food Chemistry. 49:969-974. https://doi.org/10.1021/jf001206m
  39. Wu VCH, Qiu X, Bushway A and Harper L. (2008). Antibacterial effects of American cranberry(Vaccinium macrocarpon) concentrate on foodborne pathogens. LWT-Food Science and Technology. 41:1834-1841. https://doi.org/10.1016/j.lwt.2008.01.001
  40. Wu X, Gu L, Prior RL and McKay S. (2004). Characterization of anthocyanins and proanthocyanidins in some cultivars of Ribes, Aronia, and Sambucus and their antioxidant capacity. Journal of Agricultural and Food Chemistry. 52:7846-7856. https://doi.org/10.1021/jf0486850
  41. Yamaguchi T, Takamura H, Matoba T and Teral J. (1998). HPLC method for evaluation of the free radical-scavenging activity of foods by using 1,1-diphenyl-2-picrylhydrazyl. Bioscience, Biotechnology, and Biochemistry. 62:1201-1204. https://doi.org/10.1271/bbb.62.1201
  42. Yan X, Murphy BT, Hammond GB, Vinson JA and Neto CC. (2002). Antioxidant activities and antitumor screening of extracts from cranberry fruit(Vaccinium macrocarpon). Journal of Agricultural and Food Chemistry. 50:5844-5849. https://doi.org/10.1021/jf0202234
  43. Yang SA, Pyo BS, Kim SM and Lee KI. (2012). Antibacterial activity and nitric oxide production inhibitory activity of the extract and its fractions from the leaves of Prunus sargentii. Korean Journal of Medicinal Crop Science. 20:308-314. https://doi.org/10.7783/KJMCS.2012.20.4.308
  44. Zheng W and Wang SY. (2003). Oxygen radical absorbing capacity of phenolics in blueberries, cranberries, chokeberries and lingonberries. Journal of Agricultural and Food Chemistry. 51:502-509. https://doi.org/10.1021/jf020728u

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