Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
권호 표지
DOI QR코드

DOI QR Code

Fibulin-5 is a Prognostic Marker that Contributes to Proliferation and Invasion of Human Glioma Cells

  • Sheng, Xu-Dong (Department of Neurosurgery, the First Affiliated Hospital of Xi'an Medical College) ;
  • Chen, Hu (Department of Neurosurgery, Tang-Du Hospital, Fourth Military Medical University) ;
  • Wang, Hui (Department of Neurosurgery, the First Affiliated Hospital of Xi'an Medical College) ;
  • Ding, Zhi-Bin (Department of Neurosurgery, the First Affiliated Hospital of Xi'an Medical College) ;
  • Xu, Gang-Zhu (Department of Neurosurgery, the First Affiliated Hospital of Xi'an Medical College) ;
  • Zhang, Jun-Feng (Department of Neurosurgery, the First Affiliated Hospital of Xi'an Medical College) ;
  • Lu, Wen-Chao (Department of Neurosurgery, the First Affiliated Hospital of Xi'an Medical College) ;
  • Wu, Tao (Department of Neurosurgery, the First Affiliated Hospital of Xi'an Medical College) ;
  • Zhao, Ling (Department of Anesthesiology, the First Affiliated Hospital of Xi'an Medical College)
  • Published : 2015.02.25
Download PDF
⟨ Previous Next ⟩

Abstract

Fibulin-5 has recently been considered as a potential tumor suppressor in human cancers. Several studies have shown that it is down-regulated in a variety of tumor types and inhibits tumor growth and metastasis. This study was aimed to investigate the clinical significance of fibulin-5 in glioma and its role in cell proliferation and invasion. We found that the expression of fibulin-5 in glioma tissues was significantly lower than those in normal brain (NB) tissues. Negative expression was significantly correlated with advanced clinical stage (grade III+IV). Furthermore, Fibulin-5 negative expression was correlated with a shorter overall survival of glioma patients. Multivariate Cox repression analysis indicated that fibulin-5 was an independent factor for predicting overall survival of glioma patients. Overexpression obviously inhibited cell proliferation in U251 and U87 cells. Furthermore, it significantly reduced the number of migrating and invading glioma cells. In conclusion, impaired expression of fibulin-5 is correlated with the advanced tumor stage in glioma. Otherwise, Fibulin-5 is an independent prognostic marker for predicting overall survival of glioma patients. Mechanistically, it may function as a tumor suppressor via inhibiting cell proliferation and invasion in gliomas.

Keywords

References

  1. Albig AR, Schiemann WP (2005). Fibulin-5 function during tumorigenesis. Future Oncol, 1, 23-35. https://doi.org/10.1517/14796694.1.1.23
  2. Argraves WS, Greene LM, Cooley MA, et al (2003). Fibulins: physiological and disease perspectives. EMBO Rep, 4, 1127-31. https://doi.org/10.1038/sj.embor.7400033
  3. Brat DJ, Scheithauer BW, Fuller GN, et al (2007). Newly codified glial neoplasms of the 2007 WHO Classification of Tumours of the Central Nervous System: angiocentric glioma, pilomyxoid astrocytoma and pituicytoma. Brain Pathol, 17, 319-24. https://doi.org/10.1111/j.1750-3639.2007.00082.x
  4. de Vega S, Iwamoto T, Yamada Y (2009). Fibulins: multiple roles in matrix structures and tissue functions. Cell Mol Life Sci, 66, 1890-902. https://doi.org/10.1007/s00018-009-8632-6
  5. Gu WT, Gu SX, Shou JJ (2014). Pathway and network analysis in glioma with the partial least squares method. Asian Pac J Cancer Prev, 15, 3145-9. https://doi.org/10.7314/APJCP.2014.15.7.3145
  6. Hagemann C, Fuchs S, Monoranu CM, et al (2013). Impact of MACC1 on human malignant glioma progression and patients' unfavorable prognosis. Neuro Oncol, 15, 1696-709. https://doi.org/10.1093/neuonc/not136
  7. Hu Z, Ai Q, Xu H, et al (2011). Fibulin-5 is down-regulated in urothelial carcinoma of bladder and inhibits growth and invasion of human bladder cancer cell line 5637. Urol Oncol, 29, 430-5. https://doi.org/10.1016/j.urolonc.2009.06.004
  8. Hwang CF, Shiu LY, Su LJ, et al (2013). Oncogenic fibulin-5 promotes nasopharyngeal carcinoma cell metastasis through the FLJ10540/AKT pathway and correlates with poor prognosis. PLoS One, 8, 84218. https://doi.org/10.1371/journal.pone.0084218
  9. Li C, Yang W, Zhang J, et al (2014a). SREBP-1 has a prognostic role and contributes to invasion and metastasis in human hepatocellular carcinoma. Int J Mol Sci, 15, 7124-38. https://doi.org/10.3390/ijms15057124
  10. Li F, Xu H, Zeng Y, et al (2012). Overexpression of fibulin-5 in retinal pigment epithelial cells inhibits cell proliferation and migration and downregulates VEGF, CXCR4, and TGFB1 expression in cocultured choroidal endothelial cells. Curr Eye Res, 37, 540-8. https://doi.org/10.3109/02713683.2012.665561
  11. Li PD, Wang XJ, Shan Q, et al (2014b). Evaluation of TAZ expression and its effect on tumor invasion and metastasis in human glioma. Asian Pac J Trop Med, 7, 757-60. https://doi.org/10.1016/S1995-7645(14)60131-0
  12. Nakamura T, Lozano PR, Ikeda Y, et al (2002). Fibulin-5/DANCE is essential for elastogenesis in vivo. Nature, 415, 171-5. https://doi.org/10.1038/415171a
  13. Noda K, Dabovic B, Takagi K, et al (2013). Latent TGF-beta binding protein 4 promotes elastic fiber assembly by interacting with fibulin-5. Proc Natl Acad Sci USA, 110, 2852-7. https://doi.org/10.1073/pnas.1215779110
  14. Ohara H, Akatsuka S, Nagai H, et al (2011). Stage-specific roles of fibulin-5 during oxidative stress-induced renal carcinogenesis in rats. Free Radic Res, 45, 211-20. https://doi.org/10.3109/10715762.2010.523702
  15. Papke CL, Yanagisawa H (2014). Fibulin-4 and fibulin-5 in elastogenesis and beyond: Insights from mouse and human studies. Matrix Biol, 37, 142-9. https://doi.org/10.1016/j.matbio.2014.02.004
  16. Schiemann WP, Blobe GC, Kalume DE, et al (2002). Contextspecific effects of fibulin-5 (DANCE/EVEC) on cell proliferation, motility, and invasion. Fibulin-5 is induced by transforming growth factor-beta and affects protein kinase cascades. J Biol Chem, 277, 27367-77. https://doi.org/10.1074/jbc.M200148200
  17. Tu K, Dou C, Zheng X, et al (2014). Fibulin-5 inhibits hepatocellular carcinoma cell migration and invasion by down-regulating matrix metalloproteinase-7 expression. BMC Cancer, 14, 938. https://doi.org/10.1186/1471-2407-14-938
  18. Wlazlinski A, Engers R, Hoffmann MJ, et al (2007). Downregulation of several fibulin genes in prostate cancer. Prostate, 67, 1770-80. https://doi.org/10.1002/pros.20667
  19. Xiao W, Zhou S, Xu H, et al (2013). Nogo-B promotes the epithelial-mesenchymal transition in HeLa cervical cancer cells via Fibulin-5. Oncol Rep, 29, 109-16.
  20. Yanagisawa H, Schluterman MK, Brekken RA (2009). Fibulin-5, an integrin-binding matricellular protein: its function in development and disease. J Cell Commun Signal, 3, 337-47. https://doi.org/10.1007/s12079-009-0065-3