Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
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Parecoxib: an Enhancer of Radiation Therapy for Colorectal Cancer

  • Xiong, Wei (Department of Radiation Oncology, Yunnan Cancer Hospital, The Third Affiliated Hospital of Kunming Medical University) ;
  • Li, Wen-Hui (Department of Radiation Oncology, Yunnan Cancer Hospital, The Third Affiliated Hospital of Kunming Medical University) ;
  • Jiang, Yong-Xin (Cancer Research Institute of Yunnan Province, Yunnan Cancer Hospital, The Third Affiliated Hospital of Kunming Medical University) ;
  • Liu, Shan (Cancer Research Institute of Yunnan Province, Yunnan Cancer Hospital, The Third Affiliated Hospital of Kunming Medical University) ;
  • Ai, Yi-Qin (Department of Radiation Oncology, Yunnan Cancer Hospital, The Third Affiliated Hospital of Kunming Medical University) ;
  • Liu, Rong (Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology) ;
  • Chang, Li (Department of Radiation Oncology, Yunnan Cancer Hospital, The Third Affiliated Hospital of Kunming Medical University) ;
  • Zhang, Ming (Department of Radiation Oncology, Yunnan Cancer Hospital, The Third Affiliated Hospital of Kunming Medical University) ;
  • Wang, Xiao-Li (Department of Radiation Oncology, Yunnan Cancer Hospital, The Third Affiliated Hospital of Kunming Medical University) ;
  • Bai, Han (Department of Radiation Oncology, Yunnan Cancer Hospital, The Third Affiliated Hospital of Kunming Medical University) ;
  • Wang, Hong (Cancer Research Institute of Yunnan Province, Yunnan Cancer Hospital, The Third Affiliated Hospital of Kunming Medical University) ;
  • Zheng, Rui (Cancer Research Institute of Yunnan Province, Yunnan Cancer Hospital, The Third Affiliated Hospital of Kunming Medical University) ;
  • Tan, Jing (Department of Radiation Oncology, Yunnan Cancer Hospital, The Third Affiliated Hospital of Kunming Medical University)
  • Published : 2015.02.25
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Abstract

Background: To study the effect of parecoxib, a novel cyclooxygenase-2 selective inhibitor, on the radiation response of colorectal cancer (CRC) cells and its underlying mechanisms. Materials and Methods: Both in vitro colony formation and apoptosis assays as well as in vivo mouse xenograft experiments were used to explore the radiosensitizing effects of parecoxib in human HCT116 and HT29 CRC cells. Results: Parecoxib sensitized CRC cells to radiation in vitro with a sensitivity enhancement ratio of 1.32 for HCT116 cells and 1.15 for HT29 cells at a surviving fraction of 0.37. This effect was partially attributable to enhanced apoptosis induction by parecoxib combined with radiation, as illustrated using an in vitro apoptosis assays. Parecoxib augmented the tumor response of HCT116 xenografts to radiation, achieving growth delay more than 20 days and an enhancement factor of 1.53. In accordance with the in vitro results, parecoxib combined with radiation resulted in less proliferation and more apoptosis in tumors than radiation alone. Radiation monotherapy decreased microvessel density (MVD) and microvessel intensity (MVI), but increased the hypoxia level in xenografts. Parecoxib did not affect MVD, but it increased MVI and attenuated hypoxia. Conclusions: Parecoxib can effectively enhance radiation sensitivity in CRC cells through direct effects on tumor cells and indirect effects on tumor vasculature.

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