Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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Galangin and Kaempferol Suppress Phorbol-12-Myristate-13-Acetate-Induced Matrix Metalloproteinase-9 Expression in Human Fibrosarcoma HT-1080 Cells

  • Choi, Yu Jung (Department of Biochemistry, College of Life Science and Biotechnology, Yonsei University) ;
  • Lee, Young Hun (Department of Biochemistry, College of Life Science and Biotechnology, Yonsei University) ;
  • Lee, Seung-Taek (Department of Biochemistry, College of Life Science and Biotechnology, Yonsei University)
  • Received : 2014.08.13
  • Accepted : 2014.11.05
  • Published : 2015.02.28
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Abstract

Matrix metalloproteinase (MMP)-9 degrades type IV collagen in the basement membrane and plays crucial roles in several pathological implications, including tumorigenesis and inflammation. In this study, we analyzed the effect of flavonols on MMP-9 expression in phorbol-12-myristate-13-acetate (PMA)-induced human fibrosarcoma HT-1080 cells. Galangin and kaempferol efficiently decreased MMP-9 secretion, whereas fisetin only weakly decreased its secretion. Galangin and kaempferol did not affect cell viability at concentrations up to $30{\mu}M$. Luciferase reporter assays showed that galangin and kaempferol decrease transcription of MMP-9 mRNA. Moreover, galangin and kaempferol strongly reduce $I{\kappa}B{\alpha}$ phosphorylation and significantly decrease JNK phosphorylation. These results indicate that galangin and kaempferol suppress PMA-induced MMP-9 expression by blocking activation of NF-${\kappa}B$ and AP-1. Therefore, these flavonols could be used as chemopreventive agents to lower the risk of diseases involving MMP-9.

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References

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