The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology (한방안이비인후피부과학회지)

The Society of Korean Medicine Ophthalmology, Otolaryngology & Dermatology (대한한방안이비인후피부과학회)
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Effects of Gamijaungo on the burn mice model and the study of hematologic, pathologic and molecular mechanism

가미자운고(加味紫雲膏)가 mouse의 피부화상 치료에 대한 분자생물학적 효과 및 기전연구

  • Lee, Jong-Chul (Department of Ophthalmology, Otolaryngology and Dermatology, College of Korean Medicine, Gachon University) ;
  • Kim, Gyung-Jun (Department of Ophthalmology, Otolaryngology and Dermatology, College of Korean Medicine, Gachon University)
  • 이종철 (가천대학교 한의과대학 한방안이비인후피부과) ;
  • 김경준 (가천대학교 한의과대학 한방안이비인후피부과)
  • Received : 2015.01.07
  • Accepted : 2015.02.04
  • Published : 2015.02.25
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Abstract

Objective: The aim of this study was to investigate the wound healing effect of herbal ointment, Gamijaungo, on the burn-induced model. Reports about Gamijaungo on the wound healing effect by local application in mice model or human study have published in the several domestic or internationally, but most are anecdotal and lack solid scientific evidence. Method: We observed the morphologic and histologic changes in the burn-induced mice model. we counted white blood cell and platelet changes. we confirmed VEGF, PI3K and pAkt protein expression by Western blot analysis. Result: In this study, we observed that Gamijaungo showed strong wound healing effects in the morphologic and histologic changes in the burn-induced mice model. Also we found that the significant changes of white blood cell and platelet changes by the treatment of Gamijaungo. In molecular mechanism, we got the strong positive effect by Gamijaungo treatment on angiogenesis, a key process in the formation of the granulation tissue during wound healing. Conclusion: These findings suggest the potential use of Gamijaungo as a therapeutic in thermal burn-induced skin injuries.

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References

  1. Kang YH. Encyclopedia of Life Science. Seoul:Academic books. 2009.
  2. Kang JS. Plastic Surgery. Seoul:Kunja Publisher. 2004:471-538.
  3. Cho SH, Choi SI. A Clinical Review of 1420 Burn Cases. Journal of the Korean Surgical Society. 1998;54(3):305-12.
  4. Greenhalgy DG. The role of growth factors in wound healing. J Trauma. 1996;41(1):159. https://doi.org/10.1097/00005373-199607000-00029
  5. Htherill JR, Till GO, Bruner LH, Ward PA. Thermal injury, Intravascular Hemolysis and Toxic Oxygen Products. J Clin Invest. 1986;78:629-36. https://doi.org/10.1172/JCI112620
  6. Choi BJ. Advanced in wound care. Seoul:Korea medical book. 2002:43-91.
  7. Kaufman T, Nathan P, Levin M, Hebda PA, Eichenlaub EH, Korel B. Drug-loaded synthetic dressings: effect on contraction, epithelization, and collagen synthesis of deep second-degree experimental burns, Ann. Plast. Surg. 1985;14:420-7. https://doi.org/10.1097/00000637-198505000-00004
  8. Cho AR. Effect of Silver sulfadiazine on the Skin Cell Proliferation and Wound Healing Process in Hairless Mouse 2nd degree Burn Model. Journal of Pharmaceutical Investigation. 2002;32(2):113-7. https://doi.org/10.4333/KPS.2002.32.2.113
  9. Lee KS, Park SJ, Kim SR, Min KH, Lee KY, Coe YH, Hong SH, Kim JS, Hong SJ, Lee YC. Inhibition of VEGF blocks TGF-b1 production through a PI3K/Akt signaling pathway. Eur Respir J. 2008;31:523-31. https://doi.org/10.1183/09031936.00125007
  10. Lee KS, Park SJ, Kim SR, Park SJ, Lee HK, Park HS, Min KH, Jin SM, Lee YC. Phosphatase and tension homolog deleted on chromosome 10 (PTEN) reduces vascular endothelial growth factor expression in allergen-induced airway inflammation. Mol Pharmacol. 2006;69:1829–39. https://doi.org/10.1124/mol.106.022228
  11. Lee KS, Park SJ, Kim SR, Min KH, Jin SM, Puri KD, Lee YC. Phosphoinositide 3-kinaseδ inhibitor reduces vascular permeability in a murine model of asthma. J Allergy Clin Immunol. 2006;118:403–9. https://doi.org/10.1016/j.jaci.2006.04.041
  12. Thakker GD, Hajjar DP, Muller WA, Rosengart TK. The role of phosphatidy-linositol 3-kinase in vascular endothelial growth factor signaling. J Biol Chem. 1999;274:10002–7. https://doi.org/10.1074/jbc.274.15.10002
  13. Singer AJ, Clark RA. Cutaneous wound healing. N ENgl J Med. 1999;341:738-46. https://doi.org/10.1056/NEJM199909023411006
  14. Hassam W, Zhang R, Lin M, Warn JD, Smith PG. Coordinate expression of NGF and α -smooth muscle actin mRNA and protein in cutaneous wound tissue of developing and adult rats. Cell Tissue Res. 2000;300:97-109.
  15. Shisudomyoung. Hanbang Chiryo Backhaw. Seoul:Dongnam Publisher. 1991:412-31.
  16. Yeo EJ, Han JK, Kim YH. Effects of Atopy Cream-combined with Jawoongo Ointment on the Development of Atopic Dermatitis-like Skin Lesions in NC/Nga Mouse. J Korean Oriental Pediatrics. 2009;23(1):37-72.
  17. Yeo EJ, Han JK, Kim YH. Topical Application of Atopy cream-Jawoongo ointment(A-J) of Ointment Inhibits Biostir mite antigen cream induced Atopy Dermatitis by Local Action in NC/Nga Mice. Oriental medicine of Daejeon University. 2008;17(2):185-98.
  18. Eo KJ, Ko WS, Kim YK. The effects of Jowoongo on the artificial wound on rat skin. J Korean Med Ophthalmol Otolaryngol Dermatol. 1998;11(1):54-68.
  19. Lee YT. The effects of jawoongo mixture on wound healing. Dong-Eui university Dong-Eui Papers. 1998;28(1):547-58.
  20. Kim SK, Choi HY, Ji SY, Lee SG. An Experimental study on the Synergic Effect of Jawoongo and Gentamicin in Dermal Wound Healing and Skin Wound Infection against Staphylococcus aureus, Streptococcus pyogenes, and Pseudomonas aeruginosa. The Korea Journal of Herbology. 2004;19(4): 137-60.
  21. Yoshida T, Yoshida S, Kobayashi M, Herndon DN, Suzuki F. Pivotal advance:glycyrrhizin restores the impaired production of beta-defensins in tissues surrounding the burn area and improves the resistance of burn mice to Pseudomonas aeruginosa wound infection. J Leukoc Biol. 2010;87(1):35-41. https://doi.org/10.1189/jlb.1208760
  22. Takei M, Kobayashi M, Herndon DN, Pollard RB, Suzuki F. Glycyrrhizin inhibits the manifestations of anti-inflammatory responses that appear in association with systemic inflammatory response syndrome (SIRS)-like reactions. Cytokine. 2006;35(5-6):295-301. https://doi.org/10.1016/j.cyto.2006.10.002
  23. Nationwide College of Korean Oriental Medicine Joint Instruction Compilation Committe. Bonchohak. Seoul:Yeongrimsa. 1995:178-9, 182-3, 196-9, 540-1, 578-80.
  24. Ji KY. A Study on Histological Recuperative Effect of Burn Remedies. Korean Journal of Oriental Physiology and Pathology. 2002;16(4): 774-81.
  25. Ministry of Health & Welfare. Preparation of soft hydrogel for wound healing. 2001.
  26. Min DH, Kim DK, Lim JP, Yang JH. Transdermal Drug Deliver & Therapeutic Effect of the Preparations of Lithospermi Radix and Gardeniae Fructus Extract on the Burn & Wound Healing. Journal of Pharmaceutical Investigation. 2005;35:255-63. https://doi.org/10.4333/KPS.2005.35.4.255
  27. Bruen KJ, Campbell CA, Schooler WG, deSerres S, Cairns BA, Hultman CS, Meyer AA, Randell SH. Real-time monitoring of keratin 5 expression during burn re-epithelialization 1. Journal of Surgical Research. 2004;120:12-20. https://doi.org/10.1016/j.jss.2004.02.024
  28. Lee HJ, Ahn DH, Yu TH, Park HS. Psychological Responses in Burn Patients. Journal of Korean Neuropsychiatric Association. 1998;37(6):1158-66.
  29. Alexander JW. Mechanism of immunological suppression in burn injury. J. Trauma. 1990;30:70. https://doi.org/10.1097/00005373-199012001-00017
  30. Schall T. The cytokine Handbook. New York, Academic Press. 1999.
  31. Herberston BM, Lee YM, Cho HG, Cho OJ, Repine JE. Alveolar type 2 cell abnormalities and peroxide formation in lungs of rats given IL-1 intratracheally. Inflammation. 2000;24(4): 289-303. https://doi.org/10.1023/A:1007092529261
  32. Korean Dermatological Association Dermatology Textbook Editing Board. Textbook of Dermatology. Seoul:Ryomoongak. 1994:114-5.
  33. Shanghai University of Traditional Chinese Medicine. Surgery in Chinese Medicine. Beijing:People's medical publising house. 1999:414
  34. Son SM. Bigeupcheongeumyobang. Seoul: Daesung publisher. 1984:459.
  35. National Oriental Medical School Dermatology & Surgery Textbook Editing Board. Text of Traditional Korean Dermatology & Surgery. Busan:Sun Wo Publisher. 2007:263-70.
  36. Yu MK, Jeong DH, Sim SH, Park SY, Kim JH, Choi JH. A Literature Study on the External Treatment of a Burn. J Korean Med Ophthalmol Otolaryngol Dermatol. 2003; 16(3):38-67.
  37. Science encyclopedia publisher. Component and the use of herbs. Seoul:Ilwolbooks 1999:593-5.
  38. Min KA. Study on the antimicrobial effect of angelica gigas. Catholic University Graduate School. 1996.
  39. Kim HC. Herbal medical pharmacology. Seoul:Jipmoondang. 2001:422-7.
  40. Park WY, Jang DS, Cho HR. Screening of Antimicrobial Activity for Medicinal Herb Extracts. J. Korean Soc. Food Nutr. 1992;21(1):91-6.
  41. Kim YH, Park YS. Effect of Scutellaria baicalensis Water Extract on Antioxidative Activity and Epidermal Thickness in DNCB-induced Allergic Contact Dermatitis Animal Model. J. Korean Soc. Food Nutr. 2006;35(5):543-8. https://doi.org/10.3746/jkfn.2006.35.5.543
  42. Lee GT, Choi BT, Lee YT, Ko WS, Lee GT. The Effects of Jawoongo plus Flos Lonicerae on The Artificial Wound on Rats. J. Korean Med. 1999;20(1):132-41. https://doi.org/10.3346/jkms.2005.20.1.132
  43. Kim SC, Lee JR, Choi KI, Park SJ, Kwon YG, Beon SH. Effect of Lonicerae Flos-Skin on Contact Hypersensitivity induced by Repeat Elicitation of DNCB. The Korea Journal of Herbology. 2006;21(1):9-15.
  44. Liu Y, Petreaca M, Martins-Green M. Cell and molecular mechanism of insulin-indcued angiogenesis. J Cell Mol Med. 2009;13 (11-12):4492-504. https://doi.org/10.1111/j.1582-4934.2008.00555.x

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