Development and Reproduction (한국발생생물학회지:발생과생식)

The Korean Society of Developmental Biology (한국발생생물학회)
권호 표지
DOI QR코드

DOI QR Code

A Testa Extract of Black Soybean (Glycine max (L.) Merr.) suppresses Adipogenic Activity of Adipose-derived Stem Cells

  • Jeon, Younmi (Division of Developmental Biology and Physiology, School of Biosicences and Chemistry, Sungshin University) ;
  • Lee, Myoungsook (Dept. of Food and Nutrition, Sungshin University) ;
  • Cheon, Yong-Pil (Division of Developmental Biology and Physiology, School of Biosicences and Chemistry, Sungshin University)
  • Received : 2015.11.20
  • Accepted : 2015.12.12
  • Published : 2015.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Black soybean teata is helpful to preventing obesity through enhancing energy expenditure and suppressing accumulation in mesenteric adipose tissue. The ethanol testa-extract of Cheongja #3 black soybean (ETCBS) is also have similar effects on obesity. So far, it is not clear whether the ethanol testa extract of black soybean can have effect on the characters of subcutaneous adipose stem cells such as proliferation, activity, and adipogenicity. The doubling time was different between subcutaneous adipose-derived stem (ADS) and visceral ADS cells. By the in vitro culture and passage, the doubling time was increased both of them. The shape was not different between groups and their passages were not cause the change of shapes. In the case of visceral ADS cells, the doubling time was 62.3 h or 40.3 h in control or high fat diet administrated mice, respectively, but not modified in subcutaneous ADS cells. ETCBS administration caused of increased the doubling time from 62.3 h to 84.2 h. ETCBS had suppressive effects on the cellular activity of subcutaneous ADS cells. The intensity of Oil Red O staining was very faint in 100 and $200{\mu}g/mL$ ETCBS treated groups. The amounts of accumulated triglyceride were also significantly low in 100 and $200{\mu}g/mL$ treated groups. From these results we know that the doubling times and the effects of ETCBS are different by the anatomical origin of ADS cells. It also suggested that ETCBS may suppress the differentiation of subcutaneous ADS cells into the precursors and maturing of adipocytes.

Keywords

References

  1. Ailhaud G, Grimaldi P, Negrel R (1992) Cellular and molecular aspects of adipose tissue development. Annu Rev Nutr 12:207-233. https://doi.org/10.1146/annurev.nu.12.070192.001231
  2. Cho KM, Ha TJ, Lee YB Seo WD, Kim JY, Ryu HW, Jeong SH, Kang YM, Lee JH (2013) Soluble phenolics and antioxidant properties of soybean (Glycine max L.) cultivars with varying seed coat colours. J Funct Foods 5:1065-1076. https://doi.org/10.1016/j.jff.2013.03.002
  3. Colitti M, Grasso S. 2014. Nutraceuticals and regulation of adipocyte life: Premises or promises. Biofactors 40:398-418. https://doi.org/10.1002/biof.1164
  4. Ha TJ, Lee MH, Park CH, Pae SB, Shim KB, Ko JJ, Shin SO, Baek IY, Park KY (2010) Identification and characterization of anthocyanins in yard-long beans (Vigna unguiculata ssp. sesquipedalis L.) by high-performance liquid chromatography with diode array detection and electrospray ionization/mass spectrometry (HPLC-DAD-ESI/MS) analysis. J Agricult Food Chem 58:2571-2576. https://doi.org/10.1021/jf903883e
  5. Hausman DB, DiGirolamo M, Bartness TJ, Hausman GJ, martin RJ (2001) The biology of white adipocyte proliferation. Obes Rev 2:239-254. https://doi.org/10.1046/j.1467-789X.2001.00042.x
  6. Jeon Y, Song S, Kim H, Cheon Y (2013) Diphlorethohydroxycarmalol of Ishige okamurae and caffeine modified the expression of extracellular fibrillars during adipogenesis of mouse subcutaneous adipose derived stem cell. Dev Reprod 17:275-287. https://doi.org/10.12717/DR.2013.17.3.275
  7. Kanamoto Y, Yamashita Y, Nanba F, Yoshida T, Tsuda T, Fukuda I, Nakamura-Tsuruta S, Ashida H (2011) A black soybean seed coat extract prevents obesity and glucose intolerance by up-regulating uncoupling proteins and down-regulating inflammatory cytokines in high-fat diest-fed mice. J Agric Food Chem 59:8985-8993. https://doi.org/10.1021/jf201471p
  8. Kim HK, Kim JN, Han SN, Nam JH, Na HN, Ha TJ (2012) Black soybean anthocyanins inhibit adipocyte differentiation in 3T3-L1 cells. Nutr Res 32:770-777. https://doi.org/10.1016/j.nutres.2012.06.008
  9. Kim MJ, Yang HJ, Kim JH, Ahn CW, lee JH, Kim KS, Kwon DY (2013) Obesity-related metabolomics analysis of human subjects in lack soybean peptide intervening study by ultraperformance lipquid chromatography and quadrupole-time-of-flight mass spectrometry. J Obes 2013:1-11.
  10. Kim SY, Wi HR, Choi S, Ha TJ, Le BW, Lee M (2015) Inhibitory effect of anthocyanin-rich black soybean testa (Glycine max (L.) Merr.) on the inflammation-induced adipogenesis in a DIO mouse model. J Funct Food 14:623-633. https://doi.org/10.1016/j.jff.2015.02.030
  11. Kwon SH, Ahn IS, Kim SO, Kong CS, Chung HY, Do MS, Park KY (2007) Anti-obesity and hypolipidemic effects of black soybean anthocyanins. J Med Food 10:552-556. https://doi.org/10.1089/jmf.2006.147
  12. Lee JH, Kang NS, Shin SO, Shin SH, Lim SG, Suh DY, Baek IY, Park KY, Ha TJ (2009) Characterization of anthocyanins in the black soybean (Glycine max L.) by HPLC-DAB-ESI/MS analysis. Food Chem 112:226-231. https://doi.org/10.1016/j.foodchem.2008.05.056
  13. Obst BE, Schemmel RA, Czijka-Narins D, Merkel R (1981) Adipocyte size and number in dietary obesity resistant and susceptible rats. Am J Physiol 240:E47-53.
  14. Parlee SD, Lentz SI, Mori H, MacDougald OA (2014) Quantifying size and number of adipocytes in adipose tissue. Methods Enzymol 537:93-122. https://doi.org/10.1016/B978-0-12-411619-1.00006-9
  15. Peptan IA, Hong L, Mao JJ (2006) Comparison of osteogenic potentials of visceral and subcutaneous adipose-derived cells of rabbits. Plast Reconstr Sug 117:1462-1470. https://doi.org/10.1097/01.prs.0000206319.80719.74
  16. Pouteau E, Tumer S, Aprikian O, Hellerstein M, Moser M, Darimont C, Fay LB, Mace K (2008) Time course and dynamics of adipose tissue development in obese an lean Zucker rat pups. Int J Obes 32:648-657. https://doi.org/10.1038/sj.ijo.0803787
  17. Rayalam S, Della-Fera MA, Baile CA (2008) Phytochemicals and regulation of the adipocyte life cycle. 2008. J Nutr Biochem 19:717-726. https://doi.org/10.1016/j.jnutbio.2007.12.007
  18. Ryden M, Andersson DP, Bergstrom IB, Arner P (2014) Adipose tissue and metabolic alterations: regional differrences in fat cell size and number matter, but differently: a cross-sectional study. J Clin Endocrinol Metab 99:E1870-1876. https://doi.org/10.1210/jc.2014-1526
  19. Sato D, Kusunoki M, Seino N, Nishina A, Feng Z, Tsutsumi K, Nakamura T (2015) Black soybean extract reduces fatty acid contents in subcutaneous, but not in visceral adipose triglyceride in high-fat fed rats. Int J Food Sci Nutr 66:539-545. https://doi.org/10.3109/09637486.2015.1028907
  20. Takikawa M, Inoue S, Horio F, Tsuda T (2010) Dietary anthocyanin-rich bilberry extract ameliorates hyperglycemia and insulin sensitivity via activation of AMP-activated protein kinase in diabetic mice. J Nut 140:527-533. https://doi.org/10.3945/jn.109.118216
  21. Tsuda T, Ueno Y, Aoki H, Koda T, Horio F, Takahashi N, kawada T, Osawa T (2004) Anthocyanin enhances adipocyteline secretion and adipocyte-specific gene expression in isolated rat adipocytes. Biochem Biophys Res Comm 316:149-157. https://doi.org/10.1016/j.bbrc.2004.02.031
  22. Tsuda T, Ueno Y, Kojo H Yoshikawa T, Osawa T (2005) Gene expression profile of isolated rat adipocytes treated with anthocyanins. Biochem Biophys Acta 1733:137-147.
  23. Zou Y, Chang SKC (2011) Effect of black soybean extract on the suppression of the proliferation of human AGS gastric cancer cells via the induction of apoptosis. J Agric Food Chem 59:4597-4605. https://doi.org/10.1021/jf104945x