Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Involvement of PI3K/AKT and MAPK Pathways for TNF-α Production in SiHa Cervical Mucosal Epithelial Cells Infected with Trichomonas vaginalis

  • Yang, Jung-Bo (Department of Obstetrics and Gynecology, Chungnam National University School of Medicine) ;
  • Quan, Juan-Hua (Department of Gastroenterology, The Affiliated Hospital of Guangdong Medical College) ;
  • Kim, Ye-Eun (Department of Biomedical Science, Chungnam National University School of Medicine) ;
  • Rhee, Yun-Ee (Department of Obstetrics and Gynecology, Chungnam National University School of Medicine) ;
  • Kang, Byung-Hyun (Department of Obstetrics and Gynecology, Chungnam National University School of Medicine) ;
  • Choi, In-Wook (Department of Infection Biology, Chungnam National University School of Medicine) ;
  • Cha, Guang-Ho (Department of Infection Biology, Chungnam National University School of Medicine) ;
  • Yuk, Jae-Min (Department of Infection Biology, Chungnam National University School of Medicine) ;
  • Lee, Young-Ha (Department of Infection Biology, Chungnam National University School of Medicine)
  • Received : 2015.07.08
  • Accepted : 2015.07.30
  • Published : 2015.08.31
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Abstract

Trichomonas vaginalis induces proinflammation in cervicovaginal mucosal epithelium. To investigate the signaling pathways in $TNF-{\alpha}$ production in cervical mucosal epithelium after T. vaginalis infection, the phosphorylation of PI3K/AKT and MAPK pathways were evaluated in T. vaginalis-infected SiHa cells in the presence and absence of specific inhibitors. T. vaginalis increased $TNF-{\alpha}$ production in SiHa cells, in a parasite burden-dependent and incubation time-dependent manner. In T. vaginalis-infected SiHa cells, AKT, ERK1/2, p38 MAPK, and JNK were phosphorylated from 1 hr after infection; however, the phosphorylation patterns were different from each other. After pretreatment with inhibitors of the PI3K/AKT and MAPK pathways, $TNF-{\alpha}$ production was significantly decreased compared to the control; however, $TNF-{\alpha}$ reduction patterns were different depending on the type of PI3K/MAPK inhibitors. $TNF-{\alpha}$ production was reduced in a dose-dependent manner by treatment with wortmannin and PD98059, whereas it was increased by SP600125. These data suggested that PI3K/AKT and MAPK signaling pathways are important in regulation of $TNF-{\alpha}$ production in cervical mucosal epithelial SiHa cells. However, activation patterns of each pathway were different from the types of PI3K/MAPK pathways.

Keywords

References

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