DOI QR코드

DOI QR Code

Study on Embryo Transfer System for Production of Transgenic Pigs

  • Na, Seungwon (College of Veterinary Medicine & Research Institute of Veterinary Medicine, Chungnam National University) ;
  • Lee, Euncheol (College of Veterinary Medicine & Research Institute of Veterinary Medicine, Chungnam National University) ;
  • Kim, Ghangyong (College of Veterinary Medicine & Research Institute of Veterinary Medicine, Chungnam National University) ;
  • Min, Kyuhong (College of Veterinary Medicine & Research Institute of Veterinary Medicine, Chungnam National University) ;
  • Yu, Youngkwang (College of Veterinary Medicine & Research Institute of Veterinary Medicine, Chungnam National University) ;
  • Roy, Pantu Kumar (College of Veterinary Medicine & Research Institute of Veterinary Medicine, Chungnam National University) ;
  • Fang, Xun (College of Veterinary Medicine & Research Institute of Veterinary Medicine, Chungnam National University) ;
  • Hassan, Bahia Mohamed Salih (College of Veterinary Medicine & Research Institute of Veterinary Medicine, Chungnam National University) ;
  • Yoon, Kiyoung (Department of Companion Animal, Shingu College) ;
  • Shin, Sangtae (College of Veterinary Medicine & Research Institute of Veterinary Medicine, Chungnam National University) ;
  • Cho, Jongki (College of Veterinary Medicine & Research Institute of Veterinary Medicine, Chungnam National University)
  • 투고 : 2015.12.22
  • 심사 : 2015.12.27
  • 발행 : 2015.12.31

초록

In the last 10 years, porcine somatic cell nuclear transfer to generate transgenic pig has been performed tremendous development with introduction and knockout of many genes. However, efficiency of porcine somatic cell nuclear transfer is still low and embryo transfer (ET) is one of important step for production efficiency. In porcine ET for production of transgenic cloned pig, we can consider many of points to increase production rates. In respect of seasonality and weather, porcine ET usually is not performed in summer and winter. Cloned transgenic embryos must be transferred into reproductive tracts of recipients where embryos are located after natural fertilization with similar estrous cycle. If cloned embryos with 2~4 cell stage are transferred, they must be transferred into oviducts in periovulatory stage. Number and deposition sites of transferred cloned embryos are important. And we must compare the methods of ET between surgical and non-surgical ones in respect of production efficiency. Sow recipients after natural estrus is most preferred recipients however its cost is must be considered. Here we will review many of current studies about porcine embryo transfer to increase production efficiency of transgenic pigs and strategies for further studies.

키워드

참고문헌

  1. Angel MA, Gil MA, Cuello C, Sanchez-Osorio J, Gomis J, Parrilla I, Vila J, Colina I, Diaz M, Reixach J, Vazquez JL, Vazquez JM, Roca J and Martinez EA. 2014. An earlier uterine environment favors the in vivo development of fresh pig morulae and blastocysts transferred by a nonsurgical deep-uterine method. J. Reprod. Dev. 60(5):371-376. https://doi.org/10.1262/jrd.2014-022
  2. Blitek A, Waclawik A, Kaczmarek MM, Kiewisz J and Ziecik AJ. 2010. Effect of estrus induction on prostaglandin content and prostaglandin synthesis enzyme expression in the uterus of early pregnant pigs. Theriogenology 73(9):1244-1256. https://doi.org/10.1016/j.theriogenology.2009.12.004
  3. Estienne MJ, Harper AF, Horsley BR, Estienne CE and Knight JW. 2001. Effects of P.G. 600 on the onset of estrus and ovulation rate in gilts treated with Regu-mate. J. Anim. Sci. 79(11):2757-2761. https://doi.org/10.2527/2001.79112757x
  4. Galvin JM, Killian DB and Stewart AN. 1994. A procedure for successful nonsurgical embryo transfer in swine. Theriogenology 41(6):1279-1289. https://doi.org/10.1016/0093-691X(94)90486-3
  5. Geisert RD, Morgan GL, Zavy MT, Blair RM, Gries LK, Cox A and Yellin T. 1991. Effect of asynchronous transfer and oestrogen administration on survival and development of porcine embryos. J. Reprod. Fertil. 93(2):475-481. https://doi.org/10.1530/jrf.0.0930475
  6. Huang Y, Ouyang H, Yu H, Lai L, Pang D and Li Z. 2013. Efficiency of porcine somatic cell nuclear transfer - A retrospective study of factors related to embryo recipient and embryos transferred. Biol. Open. 2(11):1223-1228. https://doi.org/10.1242/bio.20135983
  7. Koo OJ, Kang JT, Kwon DK, Park HJ and Lee BC. 2010. Influence of ovulation status, seasonality and embryo transfer method on development of cloned porcine embryos. Reprod. Domest. Anim. 45(5):773-778. https://doi.org/10.1111/j.1439-0531.2009.01346.x
  8. Li J, Rieke A, Day BN and Prather RS. 1996. Technical note: porcine non-surgical embryo transfer. J. Anim. Sci. 74(9):2263-2268. https://doi.org/10.2527/1996.7492263x
  9. Love RJ, Evans G and Klupiec C. 1993. Seasonal effects on fertility in gilts and sows. J. Reprod. Fertil. Suppl. 48:191-206.
  10. Ma Y, Li Y, Wei H, Li Q, Fang R, Zhao R, Zhang K, Xue K, Lou Y, Dai Y, Lian L and Li N. 2009. Effects of chemical activation and season on birth efficiency of cloned pigs. Sci. China C. Life Sci. 52(7):657-664. https://doi.org/10.1007/s11427-009-0087-3
  11. Martinez EA, Angel MA, Cuello C, Sanchez-Osorio J, Gomis J, Parrilla I, Vila J, Colina I, Diaz M, Reixach J, Vazquez JL, Vazquez JM, Roca J and Gil MA. 2014. Successful non-surgical deep uterine transfer of porcine morulae after 24 hour culture in a chemically defined medium. PLoS. One 9(8):e104696. https://doi.org/10.1371/journal.pone.0104696
  12. Martinez EA, Caamano JN, Gil MA, Rieke A, McCauley TC, Cantley TC, Vazquez JM, Roca J, Vazquez JL, Didion BA, Murphy CN, Prather RS and Day BN. 2004. Successful nonsurgical deep uterine embryo transfer in pigs. Theriogenology 61(1):137-146. https://doi.org/10.1016/S0093-691X(03)00190-0
  13. Martinez MF, Kastelic JP, Colazo MG and Mapletoft RJ. 2007. Effects of estradiol on gonadotrophin release, estrus and ovulation in CIDR-treated beef cattle. Domest. Anim. Endocrinol. 33(1):77-90. https://doi.org/10.1016/j.domaniend.2006.04.009
  14. Peltoniemi OA, Love RJ, Heinonen M, Tuovinen V and Saloniemi H. 1999. Seasonal and management effects on fertility of the sow: A descriptive study. Anim. Reprod. Sci. 55(1):47-61. https://doi.org/10.1016/S0378-4320(98)00159-6
  15. Peltoniemi OA, Tast A and Love RJ. 2000. Factors effecting reproduction in the pig: Aeasonal effects and restricted feeding of the pregnant gilt and sow. Anim. Reprod. Sci. 60-61:173-184. https://doi.org/10.1016/S0378-4320(00)00092-0
  16. Schmidt M, Kragh PM, Li J, Du Y, Lin L, Liu Y, Bogh IB, Winther KD, Vajta G and Callesen H. 2010. Pregnancies and piglets from large white sow recipients after two transfer methods of cloned and transgenic embryos of different pig breeds. Theriogenology 74(7):1233-1240. https://doi.org/10.1016/j.theriogenology.2010.05.026
  17. Vajta G and Callesen H. 2012. Establishment of an efficient somatic cell nuclear transfer system for production of transgenic pigs. Theriogenology 77(7):1263-1274. https://doi.org/10.1016/j.theriogenology.2011.10.040
  18. Wilde MH, Xie S, Day ML and Pope WF. 1988. Survival of small and large littermate blastocysts in swine after synchronous and asynchronous transfer procedures. Theriogenology 30(6):1069-1074. https://doi.org/10.1016/0093-691X(88)90281-6
  19. Xue JL, Dial GD, Marsh WE and Davies PR. 1994. Multiple manifestations of season on reproductive performance of commercial swine. J. Am. Vet. Med. Assoc. 204(9):1486-1489.
  20. Yoshioka K, Noguchi M, Suzuki C. 2012. Production of piglets from in vitro-produced embryos following non-surgical transfer. Anim. Reprod. Sci. 131(1-2):23-29. https://doi.org/10.1016/j.anireprosci.2012.01.018
  21. Youngs CR. 2001. Factors influencing the success of embryo transfer in the pig. Theriogenology 56(8):1311-1320. https://doi.org/10.1016/S0093-691X(01)00632-X
  22. Ziecik AJ, Biallowicz M, Kaczmarek M, Demianowicz W, Rioperez J, Wasielak M and Bogacki M. 2005. Influence of estrus synchronization of prepubertal gilts on embryo quality. J. Reprod. Dev. 51(3):379-384. https://doi.org/10.1262/jrd.17008