Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Increase of Production Ratio of Pre-selected Superior Dairy Female Offspring by Combination of OPU derived Oocytes and X-bearing Semen

  • Kim, Seong-Su (Department of Animal Science, Division of Applied Life Science (BK21Plus), Gyeongsang National University) ;
  • Choi, Byung-Hyun (Department of Animal Science, Division of Applied Life Science (BK21Plus), Gyeongsang National University) ;
  • Lee, Kyeong-Lim (Department of Animal Science, Division of Applied Life Science (BK21Plus), Gyeongsang National University) ;
  • Jin, Jong-In (Department of Animal Science, Division of Applied Life Science (BK21Plus), Gyeongsang National University) ;
  • Suh, Tae-Kwang (Korea Sexing Biotech Inc.) ;
  • Son, Cheol-Ho (Korea Sexing Biotech Inc.) ;
  • Park, Chan-Ho (Korea Sexing Biotech Inc.) ;
  • Shin, Seung-Oh (Dairy Cattle Improvement Center, National Agricultural Cooperation Federation) ;
  • Han, Kwang-Jin (Dairy Cattle Improvement Center, National Agricultural Cooperation Federation) ;
  • Lim, Hyun-Tae (Department of Animal Science, Division of Applied Life Science (BK21Plus), Gyeongsang National University) ;
  • Cho, Kyu-Woan (College of Veterinary Medicine, Gyeongsang National University) ;
  • Kong, Il-Keun (Department of Animal Science, Division of Applied Life Science (BK21Plus), Gyeongsang National University)
  • Received : 2015.04.14
  • Accepted : 2015.06.17
  • Published : 2015.06.30
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Abstract

This study was designed to evaluate the possibility of increase through dairy female offspring's ratio by transfer of pre-selected transferrable blastocyst that was produced by pre-selected X-bearing semen with OPU derived oocytes. Elite dairy female cow is demanded strongly compared with male, the so called, farmer wants to produce only an elite female dairy offspring as a candidate female dairy cow for producing milk. In our study, we selected 2 elite dairy bull semen from National Agricultural Cooperative Federation to pre-select X-bearing semen and 5 elite dairy female cows as donor for collecting of OPU derived oocytes. OPU derived embryo production system was carried out an aspiration of immature oocytes from 5 donor cows 2 times per week, total 200 times for 2 to 7 months by an ultrasonographic guided follicular aspiration system and then produced in vitro-produced blastocysts by in vitro maturation, fertilization and culture. Dairy donor semen selected H-319, 320 bull in National Agricultural Cooperative federation was sorted X-bearing semen by flow-cytometer and frozen for using IVF with OPU derived oocytes. Donor cows were selected 5 elite dairy cows from Gyeongju Dairy Cow Community and then disease tests such as 4 kinds of disease before selecting was checked. Oocyte proportion of grade 1 to 3 from total collected oocytes was significantly lower in donor A and B than those in donor C, D and E (82.16 and 70.03% vs. 90.0, 91.78 and 93.57%), respectively (p<0.05). However, number of oocytes per session in donor A, C and E was significantly higher than those in donor B and D ($7.77{\pm}3.26$, $5.85{\pm}2.10$ and $7.03{\pm}2.14$ vs. $4.68{\pm}2.61$ and $5.21{\pm}1.97$ oocytes), but donor A was significantly higher than donor C (p<0.05). Development to blastocyst in donor B, C and E was significantly higher than those in donor A and D (31.0, 25.0 and 25.0% vs. 14.3 and 4.5%), but donor A was not different in donor C and E (p<0.05). Nine out of 10 blastocysts (90.0%) derived from OPU blastocysts were confirmed male embryos that was induced with Y-bearing semen to confirm sex ratio only. Total 96 blastocysts derived from female bearing semen were transferred into synchronized recipients and then confirmed 42 recipients (43.8%) pregnancy rate, 36 offspring (37.5%) and 91.7% female sex ratio (33 female vs. 3 male offspring). Taken together all data, elite dairy female offspring could be produced effectively by in vitro production system between pre-selected x-bearing semen and OPU derived oocytes that would be influential breeder in the breeding of dairy farm to increase effectively elite dairy offspring ratio as well as net income in the dairy farmer.

Keywords

References

  1. Amann RP. 1999. Issues affecting commercialization of sexed semen. Theriogenology 52:1441-1457 (10.1016/S0093-691X(99)00229-0).
  2. Baruselli PS, Souza AH, Martins CM, Gimenes LU, Sales JNS, Ayres H, Andrade AFC, Raphael CF and Arruda RP. 2007. Semen sexado: Inseminacao artificial e transferência de embrioes. Rev. Bras. Reprod. Anim. 31:374-81.
  3. Bousquet D, Twagiramungu H, Morin N, Brisson C, Carboneau G and Durocher J. 1999. In vitro embryo production in the cow: An effective alternative to the conventional embryo production approach. Theriogenology 51:59-70 (10.1016/ S0093-691X(98)00231-3).
  4. Callesen H, Greve T and Christensen F. 1987. Ultrasonically guided aspiration of bovine follicular oocytes. Theriogenology 27:217 (10.1016/0093-691X(87)90094-X).
  5. Chebel RC, Guagnini FS, Santos JEP, Fetrow JP and Lima JR. 2010. Sex-sorted semen for dairy heifers: Effects on reproductive and lactational performances. J. Dairy Sci. 93:2496- 2507 (10.3168/jds.2009-2858).
  6. Cran DG, Johnson LA and Polge C. 1995. Sex preselection in cattle: A field trial. Vet. Rec. 136:495-496 (10.1136/vr.136.19.495).
  7. Cran DG, Johnson LA, Miller NG, Cochrane D and Polge C. 1993. Production of bovine calves following separation of X- and Y-chromosome bearing sperm and in vitro fertilization. Vet. Rec. 132:40-1 (10.1136/vr.132.2.40).
  8. De Vries A, Overton M, Fetrow J, Leslie K, Eicker S and Rogers G. 2008. Exploring the impact of sexed semen on the structure of the dairy industry. J. Dairy Sci. 91:847-856 (10.3168/jds.2007-0536).
  9. Dey SR, Deb GK, Ha AN, Lee JI, Bang JI, Lee KL and Kong IK. 2012. Co-culturing denuded oocytes during the in vitro maturation of bovine cumulus oocyte complexes exerts a synergistic effect on embryo development. Theriogenology 77:1064-77 (10.1016/j.theriogenology.2011.10.009).
  10. Faber DC, Molina JA, Ohlrichs CO, Vander Zwaag DF and Ferre LB. 2003. Commercialization of animal biotechnology. Theriogenology 59:125-38 (10.1016/S0093-691X(02)01264-5).
  11. Figueiredo RA, Barros CM, Pinheiro OL and Soler JM. 1997. Ovarian follicular dynamics in Nelore breed (Bos indicus) cattle. Theriogenology 47:1489-1505 (10.1016/S0093-691X (97)00156-8).
  12. Garcia A and Salaheddine M. 1998. Effects of repeated ultrasound- guided transvaginal follicular aspiration on bovine oocyte recovery and subsequent follicular development. Theriogenology 50:575-585 (10.1016/S0093-691X(98)00162-9).
  13. Garner DL and Seidel GE. 2008. History of commercializing sexed semen for cattle. Theriogenology 69:886-895 (10. 1016/j.theriogenology.2008.01.006). https://doi.org/10.1016/j.theriogenology.2008.01.006
  14. Jin JI, Choi BH, Kim SS, Jo HT, Sun DW, Lim HT, Lee JG, Min CS and Kong IK. 2014. Transplantation and production of OPU derived Hanwoo IVF embryos. J. Emb. Trans. 29:273-81(10.12750/JET.2014.29.3.273).
  15. Johnson LA. 2000. Sexing mammalian sperm for production of offspring: The state-of-the-art. Anim. Reprod. Sci. 60/61:93- 107 (10.1016/S0378-4320(00)00088-9).
  16. Kruip TAM, Pieterse MC, van Beneden TH, Vos PL, Wurth YA and Taveme MA. 1991. A new method for bovine embryo production: A potential alternative to superovulation. Vet. Rec. 128:208-210 (10.1136/vr.128.9.208).
  17. Lim HT, Lee SH, Yoo CK, Sun DW, Cho IC, Yoon DH, Yang DY, Cheong IC, Lee JG and Jeon JT. 2010. Establishment of high-throughput hair roots DNA isolation system and verification of its applicability for Hanwoo traceability using the 11 microsatellite makes. J. Agric. & Life Sci. 44:91-99.
  18. Lu KH and Seidel GE. 2004. Effects of heparin and sperm concentration on cleavage and blastocyst development rates of bovine oocytes inseminated with flow-cytometrically sorted sperm. Theriogenology 62:819-30 (10.1016/j.theriogenology.2003.12.029).
  19. Lu KH, Cran DG and Seidel GE. 1999. In vitro fertilization with flow-cytometrically sorted bovine sperm. Theriogenology 52:1393-1405 (10.1016/S0093-691X(99)00225-3).
  20. Parrish JJ, Susko-Parrish JL, Leibfried-Rutledge ML, Critser ES, Eyestone WH and First NL. 1986. Bovine in vitro fertilization with frozen-thawed semen. Theriogenology 25:591-600 (10.1016/0093-691X(86)90143-3).
  21. Pieterse MC, Vos PLAM, Kruip TAM, Wurth YA, van Beneden TH, Willemse AM and Taveme MAM. 1991. Transvaginal ultrasound guided follicular aspiration of bovine oocytes. Theriogenology 35:857-862 (10.1016/0093-691X(91)90426-E).
  22. Pontes JHF, Silva KCF, Basso AC, Rigo AG, Ferreira CR, Santos GMG, Sanches BV, Porcionato JPF, Vieira PHS, Faifer FS, Sterza FAM and Seneda MM. 2010. Large-scale in vitro embryo production and pregnancy rates from Bos taurus, Bos indicus, and indicus-taurus dairy cows using sexed sperm. Theriogenology 74:1349-1355 (10.1016/j.theriogenology. 2010.06.004).
  23. Rath D, Barcikowski S, De Graaf S, Garrels W, Grossfeld R, Klein S, Knabe W, Knorr C, Kues W, Meyer H, Michl J, Moench-Tegeder G, Rehbock C, Taylor U and Washausen S. 2013. Sex selection of sperm in farm animals: Status report and developmental prospects. Reproduction 145:R15- R30 (10.1530/REP-12-0151).
  24. Rosenkrans CF, Zeng GQ, McNamara GT, Schoff PK and First NL. 1993. Development of bovine embryos in vitro as affected by energy substrates. Biol. Reprod. 49:459-62 (10. 1095/biolreprod49.3.459). https://doi.org/10.1095/biolreprod49.3.459
  25. Sartori R, Souza AH, Guenther JN, Caraviello DZ, Geiger LN, Schenk JL and Wiltbank MC. 2004. Fertilization rate and embryo quality in superovulated Holstein heifers artificially inseminated with X-sorted or unsorted sperm. Anim. Reprod. 1:86-90.
  26. Schenk JL, Suh TK and Seidel GE. 2006. Embryo production from superovulated cattle following insemination of sexed sperm. Theriogenology 65:299-307 (10.1016/j.theriogenology.2005.04.026).
  27. Schenk JL, Suh TK, Cran DG and Seidel GE. 1999. Cryopreservation of flow-sorted bovine spermatozoa. Theriogenology 52:1375-1391 (10.1016/S0093-691X(99)00224-1).
  28. Segerson EC, Hansen TR, Libby DW, Randel RD and Getz WR. 1984. Ovarian and uterine morphology and function in Angus and Brahman cows. J. Anim. Sci. 59:1026-1046 (10. 2134/jas1984.5941026x). https://doi.org/10.2527/jas1984.5941026x
  29. Seidel GE. 2003. Economics of selecting for sex: The most important genetic trait. Theriogenology 59:585-598 (10.1016/ S0093-691X(02)01242-6).
  30. Seidel GE. 2007. Overview of sexing sperm. Theriogenology 68:443-446 (10.1016/j.theriogenology.2007.04.005).
  31. Seidel GE, Schenk JL, Herickhoff LA, Doyle SP, Brink Z, Green RD and Cran DG. 1999. Insemination of heifers with sexed sperm. Theriogenology 52:1407-1420 (10.1016/S00- 93-691X(99)00226-5).
  32. Seidel GE. 2010. Brief introduction to whole genome selection in cattle using single nucleotide polymorphisms. Reprod. Fertil. Dev. 22:138-144 (10.1071/RD09220).
  33. Stroud B. 2011. IETS Statistics and Data Retrieval Committee Report. The year 2011 worldwide statistics of embryo transfer in domestic farm animals. IETS Newsletter.
  34. Suh TK and Schenk JL. 2003. Pressure during flow sorting of bull sperm affects post-thaw motility characteristics. Theriogenology 59:516 (Abstr.).
  35. Taylor STCS, Moore AJ and Thiessen RB. 1985. Efficiency of food utilization in traditional and sex-controlled systems of beef production. Anim. Prod. 40:401-440 (10.1017/S000- 3356100040125).
  36. Teepker G and Keller DS. 1989. Selection of sires originating from a nucleus breeding unit for use in a commercial dairy population. Can. J. Anim. Sci. 69:595-604 (10.4141/cjas- 89-071).
  37. Tominaga K and Hamada Y. 2004. Efficient production of sexidentified and cryosurvived bovine in-vitro produced blastocysts. Theriogenology 61:1181-1191 (10.1016/j.theriogenology.2003.07.008).
  38. Viana JHM, Ferreira AM, Sa WF and Camargo LSA. 2000. Follicular dynamics in Zebu cattle. Pesq Agrop Bras. 35: 2501-2509 (10.1590/S0100-204X2000001200021).
  39. Wilson RD, Weigel KA, Fricke PM, Rutledge JJ, Leibfried- Rutledge ML, Matthews DL and Schutzkus VR. 2005. In vitro production of Holstein embryos using sex-sorted sperm and oocytes from selected cull cows. J. Dairy Sci. 88:776- 782 (10.3168/jds.S0022-0302(05)72741-7).
  40. Wilson RD, Fricke PM, Leibfried-Rutledge ML, Rutledge JJ, Penfield CM and Weigel KA. 2006. In vitro production of bovine embryos using sex-sorted sperm. Theriogenology 65: 1007-1015 (10.1016/j.theriogenology.2005.07.007).
  41. Yang XY, Zhao JG, Li H, Liu HF, Huang Y, Huang SZ, Zeng F and Zeng YT. 2008. Effect of individual heifer oocyte donors on cloned embryo development in vitro. Anim. Reprod. Sci. 104:28-37 (10.1016/j.anireprosci.2007.01.017).
  42. Yang XY, Zhao JG, Li HW, Li H, Liu HF, Huang SZ and Zeng YT. 2005. Improving in vitro development of cloned bovine embryos with hybrid (Holstein-Chinese Yellow) recipient oocytes recovered by ovum pick up. Theriogenology 64:1263-72 (10.1016/j.theriogenology.2004.11.026).