Journal of Korean Traditional Oncology (대한암한의학회지)

Korean Association of Traditional Oncology (대한암한의학회)
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Anti-tumor Activities of Onbaekwon on Various Cancer Cells

온백원이 암세포에 미치는 항암활성 효과

  • Lee, Jee Young (Department of Korean Internal Medicine, Kyung Hee University Hospital at Gangdong) ;
  • Oh, Hye Kyung (Department of Korean Internal Medicine, Kyung Hee University Hospital at Gangdong) ;
  • Ryu, Han Sung (Department of Korean Internal Medicine, Kyung Hee University Hospital at Gangdong) ;
  • Kim, Nam Jae (East-West Medical Research Institute, Kyung Hee University Medical Center) ;
  • Jung, Won-Yong (East-West Medical Research Institute, Kyung Hee University Medical Center) ;
  • Oh, Hyun-A (East-West Medical Research Institute, Kyung Hee University Medical Center) ;
  • Choi, Hyuck Jai (East-West Medical Research Institute, Kyung Hee University Medical Center) ;
  • Yoon, Seong Woo (Department of Korean Internal Medicine, Kyung Hee University Hospital at Gangdong) ;
  • Ryu, Bong-Ha (Department of Korean Internal Medicine, Kyung Hee University Medical Center)
  • 이지영 (강동경희대학교병원 한방암센터 한방내과) ;
  • 오혜경 (강동경희대학교병원 한방암센터 한방내과) ;
  • 류한성 (강동경희대학교병원 한방암센터 한방내과) ;
  • 김남재 (경희대학교 동서의학연구소 약물연구부) ;
  • 정원용 (경희대학교 동서의학연구소 약물연구부) ;
  • 오현아 (경희대학교 동서의학연구소 약물연구부) ;
  • 최혁재 (경희대학교 동서의학연구소 약물연구부) ;
  • 윤성우 (강동경희대학교병원 한방암센터 한방내과) ;
  • 류봉하 (경희대학교 한방병원 비계내과)
  • Received : 2015.11.15
  • Accepted : 2015.12.20
  • Published : 2015.12.30
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Abstract

Objective : The objective of this study was to investigate the experimental efficacy of anti-tumor activity of the complexed herbal formula, Onbaekwon (OBW), which was derived from the literature of Traditional Korean Medicine, Dongeuibogam. Methods : Nine Cancer cell lines, LoVo, MCF-7, HepG2, AGS, A549, NCI-H69, HL-60, Sarcoma 180, LL/2, were prepared and the cytotoxicity was assessed by 3-(4,5-dimethylthiazol-2yl)-2,5-dephenyl tetrazolium bromide (MTT) assay. Four of them, NCI-H69, HL-60, Sarcoma 180, and LL/2, showed strong cytotoxic activities and they were additionally undergone flow cytometry to find out their effects on apoptosis. ICR male mice were implanted with Sarcoma 180 intraperitoneally and divided into 8 species for each group. Control group was treated with normal saline, positive control group was treated with cyclophosphamide 8mg/kg, and experimental group was treated with OBW 1 g/kg. Results : Among 9 cancer cell lines, NCI-H69, HL-60, Sarcoma 180, and LL/2, expressed less than 0.10 mg/ml of $IC_{50}$ under 0.1~1mg/ml of OBW. NCI-H69, HL-60, Sarcoma 180, and LL/2, showed dose-dependent efficacy of apoptosis. When Sarcoma 180 cancer cell was implanted in ICR male mice and treated with the OBW, they prolonged the median overall survival for 0.8 days, from 17.5 to 18.3. Conclusion : OBW showed strong cytotoxicity to some cancer cells, which are NCI-H69, HL-60, Sarcoma 180, and LL/2, and its apoptotic activity was dose-dependent. OBW prolonged the median survival of mice implanted with Sarcoma 180. Further researches would be expected to support the efficacy of OBW.

Keywords

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