The Korean Journal of Pesticide Science (농약과학회지)

The Korean Society of Pesticide Science (한국농약과학회)
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Development of Analytical Method for Fenoxanil in Agricultural Products Using GC-NPD and GC/MS

농산물 중 Fenoxanil 잔류성 시험법 개발

  • Kim, Gyeong-Ha (Department of Herbal Medicine Resource, Kangwon National University) ;
  • Ahn, Kyung-Geun (Department of Herbal Medicine Resource, Kangwon National University) ;
  • Kim, Gi-Ppeum (Department of Herbal Medicine Resource, Kangwon National University) ;
  • Hwang, Young-Sun (Department of Herbal Medicine Resource, Kangwon National University) ;
  • Lee, Young Deuk (Division of Life and Environmental Science, Daegu University) ;
  • Choung, Myoung-Gun (Department of Herbal Medicine Resource, Kangwon National University)
  • 김경하 (강원대학교 생약자원개발학과) ;
  • 안경근 (강원대학교 생약자원개발학과) ;
  • 김기쁨 (강원대학교 생약자원개발학과) ;
  • 황영선 (강원대학교 생약자원개발학과) ;
  • 이영득 (대구대학교 생명환경학부) ;
  • 정명근 (강원대학교 생약자원개발학과)
  • Received : 2015.10.14
  • Accepted : 2015.11.17
  • Published : 2015.12.31
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Abstract

The aim of this study is to develop residue analysis method for fenoxanil, a MBI (melanin biosynthesis inhibitor) propionamide fungicide, had mainly been used to control rice blast, and disease of other crops, fruits, and vegetables by using GLC/NPD and GC/MS. Extraction with acetone and partition with n-hexane/dichloromethane (80/20, v/v) were performed from hulled rice, soybean, Kimchi cabbage, green pepper, and apple, then column clean-up with florisil was applied. Mean recoveries were 82.2%-109.1% with less than 7.2% of coefficients of variation and limit of quantitation was set at the concentration of 0.04 mg/kg from the five agricultural products through the determination by GLC/NPD equipped with DB-5 capillary column and single laboratory validation. As a confirmatory method, GC/MS selected ion monitoring (SIM) was set from m/z 125.0, 188.9, and 293.0. Developed method is expected to apply the single residue analysis of fenoxanil in agricultural products.

본 연구는 GC/NPD와 GC/MS를 이용하여 농산물 시료에 적용 가능한 MBI propionamide계 살균제 fenoxanil의 잔류 분석법을 확립하였다. 고추, 배추, 사과, 현미 및 콩을 대표 농산물로 설정하여 fenoxanil 단성분의 정량 및 확인 시험법을 개발하였다. 각각 농산물에 대한 추출은 acetone을 이용하였으며, 분배과정은 n-hexane/dichloromethane (80/20, v/v), 정제는 florisil 흡착제를 활용한 정제법을 적용하였다. 정량분석은 DB-5 capillary column을 GC/NPD에 장착하여 수행하였고, 각 농산물에 대한 정량한계는 0.04 mg/kg으로 설정 가능하였다. 회수율은 82.2-109.1%로 조사되었으며 농산물 시료의 종류 및 처리수준에 관계없이 7.2% 미만의 변동계수를 나타냈다. 정성적 확인을 위해 GC/MS를 이용, m/z 125.0, 188.9 및 293.0을 fenoxanil의 정성확인을 위한 선택적 이온으로 설정하였다.

Keywords

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