한국식품영양과학회지 (Journal of the Korean Society of Food Science and Nutrition)

  • 제44권1호
  • /
  • Pages.24-28
  • /
  • 2015
  • /
  • 1226-3311(pISSN)
  • /
  • 2288-5978(eISSN)
한국식품영양과학회 (The Korean Society of Food Science and Nutrition)
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ICR 마우스를 이용한 Bacillus subtilis JNS 균주의 단회경구투여 독성시험

Single Dose Oral Toxicity of Bacillus subtilis JNS in ICR Mice

  • 김경훈 ((주)제이엔에스텍) ;
  • 정창화 ((주)제이엔에스텍) ;
  • 주성제 (동의대학교 생명응용학과) ;
  • 박종훈 (동의대학교 생명응용학과) ;
  • 문지영 (동의대학교 생명응용학과) ;
  • 조은지 (동의대학교 생명응용학과) ;
  • 이현태 (동의대학교 생명응용학과) ;
  • 권현주 (동의대학교 생명응용학과) ;
  • 김병우 (동의대학교 생명응용학과) ;
  • 엄성환 (한국식품연구원 소재연구센터) ;
  • 이은우 (동의대학교 생명응용학과)
  • Kim, Kyoung-Hoon (JNS Tech Co.) ;
  • Jeong, Chang Hwa (JNS Tech Co.) ;
  • Joo, Seong-Je (Department of Life Science and Biotechnology, Dongeui University) ;
  • Park, Jong-Hoon (Department of Life Science and Biotechnology, Dongeui University) ;
  • Moon, Ji-Young (Department of Life Science and Biotechnology, Dongeui University) ;
  • Cho, Eun-Ji (Department of Life Science and Biotechnology, Dongeui University) ;
  • Lee, Hyun-Tai (Department of Life Science and Biotechnology, Dongeui University) ;
  • Kwon, Hyun-Ju (Department of Life Science and Biotechnology, Dongeui University) ;
  • Kim, Byung-Woo (Department of Life Science and Biotechnology, Dongeui University) ;
  • Eom, Sung-Hwan (Food Resource Research Center, Korea Food Research Institute) ;
  • Lee, Eun-Woo (Department of Life Science and Biotechnology, Dongeui University)
  • 투고 : 2014.08.27
  • 심사 : 2014.12.08
  • 발행 : 2015.01.31
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초록

청국장에서 분리한 항균활성이 우수한 Bacillus subtilis JNS의 probiotics로서의 활용성을 검증하기 위해 단기투여에 의한 안전성을 확인하고자 실험을 실시하였다. 경구투여용 존데를 이용하여 임상적용 경로인 경구로 투여하였으며, 최고투여용량은 2,000 mg/kg으로 설정하였다. ICR계 암수 마우스에서 14일간 관찰한 결과 일반증상, 사망률, 체중, 임상증상 및 육안적 소견 등에서 무처리 대조군과 비교해서 유의성 있는 차이를 보이지 않았다. 부검 후의 주요 장기에 대한 육안적 검사에서 또한 대조군과 비교하여 이상소견이나 병변이 관찰되지 않았다. 이상의 결과로부터 시험물질인 B. subtilis JNS는 경구투여 시 ICR계 암수 마우스에서 독성학적인 변화가 관찰되지 않았으며, 경구투여가 2,000 mg/kg 이상인 저독성의 안전한 물질로 사료된다.

The present study was carried out to investigate the in vivo single-dose acute toxicity of Bacillus subtilis JNS isolated from Cheonggukjang, which is a probiotic candidate showing strong and broad antibacterial activity. The test sample was orally administrated to male and female ICR mice at a highest dose of 2,000 mg/kg for 14 days. No significant change in general conditions, mortalities, body weight changes, clinical signs, autopsy findings, or presence of gross lesions was observed in either sex of mice. The results indicate that up to 2,000 mg/kg of B. subtilis JNS had no adverse effect on ICR mice.

키워드

참고문헌

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피인용 문헌

  1. Genotoxicological Safety Evaluation of Crude Antifungal Compounds Produced by Bacillus subtilis SN7 vol.28, pp.1, 2017, https://doi.org/10.7856/kjcls.2017.28.1.131
  2. Single Oral Dose Toxicity Test and Four Weeks Repeated Oral Dose Determination Test of Crude Antifungal Compounds Produced by Bacillus subtilis SN7 in Rats vol.27, pp.3, 2016, https://doi.org/10.7856/kjcls.2016.27.3.437