Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Initiation of embryogenic callus from mature zygotic embryos in Japanese larch (Larix kaempferi)

  • Kim, Yong-Wook (Department of Forest Genetics, Korea Forest Research Institute)
  • Received : 2015.09.11
  • Accepted : 2015.09.23
  • Published : 2015.09.30
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Abstract

Embryogenic callus (EC) was created from mature embryos of Larix kaempferi. With the mature embryos, keeping the culture in dark conditions throughout the experiment (38.2%) seemed to give better results than exposing them to 16 h light ($25{\mu}Em^{-2}s^{-1}$) for the first week (21.9%). EC was obtained most frequently from Quoirin and Lepoivre (LP) mediums with 1.0 mg/L 4-amino-3,5,6-trichloropicolinic acid (Picloram), plus 1.0 mg/L benzyladenine (BA) (62.8%) or Litvay's medium (LM) containing 1.0 mg/L p-chlorophenoxyacetic acid (pCPA) plus 1.0 mg/L BA (62.8%) treatment. In both cases, best results were obtained when zygotic embryos were cultured in darkness. As for the effective sucrose concentration on initiation of EC, 29.2 mM sucrose (38.6%) gave the best results.

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References

  1. Attree SM, Fowke LC (1991) Conifer somatic embryogenesis. In:Bajai YPS(Ed) Biotechnology in Agriculture and Forestry, Springer-Verlag, Berlin. 17:53-70
  2. Bailian Li, Wyckoff GW (1994) Breeding strategies for Larix decidua, L. Leptolepis and their hybrids in the United States. For Genet 1:65-72
  3. Beardmore T, Charest PJ (1995) Black spruce somatic embryo germination and desiccation tolerance. I. Effects of abscisic acid, cold, and heat treatments on the germinability of mature black spruce somatic embryos. Can J For Res 25:1763-1772 https://doi.org/10.1139/x95-191
  4. Bonga JM, Pond SE (1991) Adventitious shoot formation in cultures of 30-year-old Larix decidua, L. leptolepis, L. eurolepis, and L. larcina trees. Plant Cell Tiss Org Cult 26:45-51 https://doi.org/10.1007/BF00116609
  5. Diner AM, Strickler A, Karnosky DF (1986) Initiation, elongation, and remultiplication of Larix decidua micropropagules. New Zealand J For Sci 16:306-318
  6. Einspahr DW, McDonough TJ, Joachimedes T (1983) Kraft pulping characteristics of European, Japanese, and European$\times$ Japanese larch hybrids. Tappi 66:72-76
  7. Gowere ST, Richards JH (1990) Larches: deciduous conifers in an evergreen world. Biosci 40:818-826 https://doi.org/10.2307/1311484
  8. Gupta PK, Durzan DL (1989) Plantlet regeneration via somatic embryogenesis from subcultured callus of mature embryos of Picea abies (Norway spruce). In Vitro Cell Develop Biol-Plant 22:685-688
  9. Hatton JV (1986) Chemical and pulping properties. In: Larch Workshop Proceedings. Fredericton, NB, October 14, New Brunswick Forest Research Advisor Committee. pp.17-305
  10. Klimaszewska K (1989) Plantlet developmental from immature zygotic embryos of hybrid larch through somatic embryogenesis. Plant Sci 63:95-103 https://doi.org/10.1016/0168-9452(89)90105-2
  11. Lelu MA, Bastien C, Klimaszewska K, Ward C, Charest PJ (1994a) An improved method for somatic plantlet production in hybrid larch (Larix$\times$leptoeuropaea): Part 1. Somatic embryo maturation Plant Cell Tiss Org Cult 36:107-115 https://doi.org/10.1007/BF00048321
  12. Lelu MA, Klimaszewska K, Charest PJ (1994b) Somatic embryogenesis from immature and mature zygotic embryos and from cotyledons and needles of somatic plantlets of Larix. Can J For Res 24:100-106 https://doi.org/10.1139/x94-015
  13. Liao YK, Amerson H (1995) Slash pine (Pinus elliottii Engelm) somatic embryogenesis I. Initiation of embryogenic cultures from immature zygotic embryos. New For 10:145-163
  14. Litvay JD, Verma DC, Johnson MA (1985) Influence of a loblolly pine (Pinus taeda L.) culture medium and its components on growth and somatic embryogenesis of the wild carrot (Darcus carota L.) Plant Cell Rep 4:325-328 https://doi.org/10.1007/BF00269890
  15. Lu CV, Thorpe TA (1986) Somatic embryogenesis and plantlet regeneration in cultured immature embryos of Picea glauca. J Plant Physiol 128:297-302
  16. Nagmani R, Bonga JM (1985) Embryogenesis in subcultured callus of Larix decidua. Can J For Res 15:1088-1091 https://doi.org/10.1139/x85-177
  17. Quoirin M, Lepoivre P (1977) Etude de milleux adates aux cultures in vitro de Prunus. Acta Hortic 78:439-432
  18. Schenk RV, Hildebrandt AC (1972) Medium and techniques for induction and growth of monocotyledonous plant cell cultures. Can J Bot 50:199-204 https://doi.org/10.1139/b72-026
  19. Thompson RG, von Aderkas P (1992) Somatic embryogenesis and plant regeneration from immature embryos of western larch. Plant Cell Rep 11:379-385 https://doi.org/10.1007/BF00234365
  20. Verhagen SA, Wana SR (1989) Norway spruce somatic embryogenesis: high-frequency initiation from light-cultured mature embryos. Plant Cell Tiss Org Cult 16:103-111 https://doi.org/10.1007/BF00036518
  21. von Aderkas P, Bonga JM (1987) Promotion of embryogenesis in cultured megagametophytes of Larix decidua. Can J For Res 17:1293-1296 https://doi.org/10.1139/x87-200
  22. von Aderkas P, Bonga JM (1988) Formation of haploid embroids of Larix deciuda : early embryogenesis. Am J Bot 75:690-700. https://doi.org/10.2307/2444202
  23. von Aderkas P, Klimaszewska K, Bonga JM (1990) Diploid and haploid embryogenesis in Larch leptolepis, L. decidua, and their reciprocal hybrids. Can J For Res 20:9-14 https://doi.org/10.1139/x90-002
  24. von Arnold S, Hakman I (1986) Effects of sucrose on initiation of embryogenic callus cultures from mature zygotic embryos of Picea abies (L) Karst (Norway spruce). J Plant Physiol 122:261-265 https://doi.org/10.1016/S0176-1617(86)80125-0

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