Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Alteration of plant hormones in transgenic rice (Oryza sativa L.) by overexpression of anti-apoptosis genes during salinity stress

  • Ubaidillah, Mohammad (Division of Plant Biosciences, School of Applied Biosciences, College of Agriculture & Life Science, Kyungpook National University) ;
  • Safitri, Fika Ayu (Division of Plant Biosciences, School of Applied Biosciences, College of Agriculture & Life Science, Kyungpook National University) ;
  • Lee, Sangkyu (Research Institute of Pharmaceutical Sciences, College of Pharmacy, Kyungpook National University) ;
  • Park, Gyu-Hwan (School of Ecology & Environmental System, College of Ecology & Environmental Science, Kyungpook National University) ;
  • Kim, Kyung-Min (Division of Plant Biosciences, School of Applied Biosciences, College of Agriculture & Life Science, Kyungpook National University)
  • Received : 2015.08.26
  • Accepted : 2015.09.21
  • Published : 2015.09.30
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Abstract

We previously identified the rice gene, OsSAP, as an encoder of a highly conserved putative senescence-associated protein that was shown to have anti-apoptotic activity. To confirm the role of OsSAP in inducing abiotic stress tolerance in rice, we introduced OsSAP and AtBI-1, a plant homologue of Bax inhibitor-1, under the control of the CaMV 35S promoter into the rice genome through Agrobacterium-mediated transformation. The OsSAP transformants showed a similar chlorophyll index after salinity treatments with AtBI-1. Furthermore, we compared the effects of salinity stress on leaves and roots by examining the hormone levels of abscisic acid (ABA), jasmonic acid (JA), gibberellic acid (GA3), and zeatin in transformants compared to the control. With the exception of phytohormones, stress-induced changes in hormone levels putatively related to stress tolerance have not been investigated previously. Hormonal level analysis confirmed the lower rate of stress in the transformants compared to the control. The levels of ABA and JA in OsSAP and AtBI-1 transformants were similar, where stress rates increased after one week and decreased after a two week period of drought; there was a slightly higher accumulation compared to the control. However, a similar trend was not observed for the level of zeatin, as the decrease in the level of zeatin accumulation differed in both OsSAP and AtBI-1 transformants for all genotypes during the early period of salinity stress. The GA3 level was detected under normal conditions, but not under salinity stress.

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References

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