Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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Direct Regulation of TLR5 Expression by Caveolin-1

  • Lim, Jae Sung (Department of Biochemistry, Chonnam National University Medical School) ;
  • Nguyen, Kim Cuc Thi (Department of Biochemistry, Chonnam National University Medical School) ;
  • Han, Jung Min (Department of Integrated OMICS for Biomedical Science, Yonsei University) ;
  • Jang, Ik-Soon (Division of life Science, Korea Basic Science Institute) ;
  • Fabian, Claire (Department of Immunology, Fraunhofer Institute for Cell Therapy and Immunology (IZI)) ;
  • Cho, Kyung A (Department of Biochemistry, Chonnam National University Medical School)
  • Received : 2015.08.03
  • Accepted : 2015.09.25
  • Published : 2015.12.31
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Abstract

Toll-like receptor 5 (TLR5) is a specific receptor for microbial flagellin and is one of the most well-known receptors in the TLR family. We reported previously that TLR5 signaling is well maintained during aging and that caveolin-1 may be involved in TLR5 signaling in aged macrophages through direct interactions. Therefore, it is important to clarify whether caveolin-1/TLR5 interactions affect TLR5 expression during aging. To assess the effect of caveolin-1 on TLR5, we analyzed TLR5 expression in senescent fibroblasts and aged tissues expressing high levels of caveolin-1. As expected, TLR5 mRNA and protein expression was well maintained in senescent fibroblasts and aged tissues, whereas TLR4 mRNA and protein were diminished in those cells and tissues. To determine the mechanism of caveolin-1-dependent TLR5 expression, we examined TLR5 expression in caveolin-1 deficient mice. Interestingly, TLR5 mRNA and protein levels were decreased dramatically in tissues from caveolin-1 knockout mice. Moreover, overexpressed caveolin-1 in vitro enhanced TLR5 mRNA through the MAPK pathway and prolonged TLR5 protein half-life through direct interaction. These results suggest that caveolin-1 may play a crucial role in maintaining of TLR5 by regulating transcription systems and increasing protein half-life.

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References

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