DOI QR코드

DOI QR Code

Isolation and Identification of Yeasts from Jeju Island Soils

제주도 토양에서 효모의 분리 및 동정

  • Han, Sang-Min (Department of Biomedicinal Science and Biotechnology, Paichai University) ;
  • Bae, Sang-Min (Department of Biomedicinal Science and Biotechnology, Paichai University) ;
  • Han, Jae-Won (Department of Biomedicinal Science and Biotechnology, Paichai University) ;
  • Kim, Ji-Yoon (Department of Biomedicinal Science and Biotechnology, Paichai University) ;
  • Lee, Jong-Soo (Department of Biomedicinal Science and Biotechnology, Paichai University) ;
  • Kim, Ha-Kun (Department of Biomedicinal Science and Biotechnology, Paichai University)
  • 한상민 (배재대학교 바이오.의생명공학과) ;
  • 배상민 (배재대학교 바이오.의생명공학과) ;
  • 한재원 (배재대학교 바이오.의생명공학과) ;
  • 김지윤 (배재대학교 바이오.의생명공학과) ;
  • 이종수 (배재대학교 바이오.의생명공학과) ;
  • 김하근 (배재대학교 바이오.의생명공학과)
  • Received : 2015.12.05
  • Accepted : 2015.12.10
  • Published : 2015.12.31

Abstract

Significant differences in annual precipitates on Jeju island have been reported depending on the location. We collected soil samples from east and west areas of Jeju Island to identify yeasts by plating on yeast peptone dextrose plates and subsequent analysis for the polymerase chain reaction amplified D1/D2 region of 26S rDNA of colonies. As a result, 20 yeast strains of 12 species were isolated from 7 different sampling sites in east area and 13 yeast strains of 6 species from 5 different sampling sites in west area. Some differences in yeast flora were observed depending on the sample collection sites having different annual precipitates.

제주도 토양에 서식하는 야생효모들의 분포 특성을 조사하기 위해, 동부지역 7곳, 서부지역 5곳에서 토양을 채취하여 효모를 분리한 후, 콜로니 PCR에 의해 증폭된 26S rDNA D1/D2 지역의 염기서열을 결정하여 BLAST를 이용하여 효모를 동정하였다. 동부지역에서 채집한 토양에서 12종에 속하는 20균주를, 서부지역에서 6종에 속하는 13균주를 분리하였다. 분리되는 효모 종은 강수량 차이를 보이는 지역에 따라서 현저한 차이가 있는 것을 알 수 있었다.

Keywords

References

  1. Palmisano A, Benecchi M, De Filippo M, Maggiore U, Buzio C, Vaglio A. Candida sake as the causative agent of spondylodiscitis in a hemodialysis patient. Spine J 2011;11:e12-6. https://doi.org/10.1016/j.spinee.2011.01.026
  2. Bal J, Yun SH, Song HY, Yeo SH, Kim JH, Kim JM, Kim DH. Mycoflora dynamics analysis of Korean traditional wheatbased nuruk. J Microbiol 2014;52:1025-9. https://doi.org/10.1007/s12275-014-4620-0
  3. Hong SG, Lee KH, Bae KS. Diversity of yeasts associated with natural environments in Korea. J Microbiol 2002;40:55-62.
  4. Hyun SH, Han SM, Kim HK, Lee JS. Yeasts diversity of wild flowers in mountains of Korea and their physiological functionalities. Kor J Mycol 2015;43:137-41.
  5. Kim DH, Kim SH, Kwon SW, Lee JK, Hong SB. Mycoflora of soybeans used for meju fermentation. Mycobiology 2013;41:100-7. https://doi.org/10.5941/MYCO.2013.41.2.100
  6. Kurtzman CP, Robnett CJ. Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie Van Leeuwenhoek 1998;73:331-71. https://doi.org/10.1023/A:1001761008817
  7. Min JH, Kim YH, Kim JH, Choi SY, Lee JS, Kim HK. Comparison of microbial diversity of Korean commercial Makgeolli showing high $\beta$-glucan content and high antihypertensive activity, respectively. Mycobiology 2012;40:138-41. https://doi.org/10.5941/MYCO.2012.40.2.138
  8. Hyun SH, Lee JG, Park WJ, Kim HK, Lee JS. Isolation and diversity of yeasts from fruits and flowers of orchard in Sinammyeon of Yesan-gun, Chungcheongnam-do, Korea. Kor J Mycol 2014;42:21-7. https://doi.org/10.4489/KJM.2014.42.1.21
  9. Hyun SH, Mun HY, Lee HB, Kim HK, Lee JS. Isolation of yeasts from wild flowers in Gyonggi-do province and Jeju island in Korea and the production of anti-gout xanthine oxidase inhibitor. Korean J Microbiol Biotechnol 2013;41:383-90. https://doi.org/10.4014/kjmb.1309.09002
  10. Hyun SH, Han SM, Lee JS. Isolation and physiological functionality of yeasts from wild flowers in Seonyudo of Gogunsanyeoldo, Jeollabuk-do, Korea. Kor J Mycol 2014;42:201-6. https://doi.org/10.4489/KJM.2014.42.3.201
  11. Min JH, Lee HB, Lee JS, Kim HK. Identification of yeasts isolated from wild flowers collected in coast areas of Korea based on the 26S rDNA sequences. Kor J Mycol 2013;41:185-91. https://doi.org/10.4489/KJM.2013.41.3.185
  12. Min JH, Ryu JJ, Kim HK, Lee JS. Isolation and identification of yeasts from wild flowers in Gyejoksan, Oseosan and Beakamsan of Korea. Kor J Mycol 2013;41:47-51. https://doi.org/10.4489/KJM.2013.41.1.47
  13. Hyun SH, Min JH, Kim SA, Lee JS, Kim HK. Yeasts associated with fruits and blossoms collected from Hanbat arboretum, Daejeon, Korea. Kor J Mycol 2014;42:178-82. https://doi.org/10.4489/KJM.2014.42.2.178
  14. Han SM, Park WJ, Lee JS. Isolation and diversity of wild yeasts from some cereals. Kor J Mycol 2015;43:64-7. https://doi.org/10.4489/KJM.2015.43.1.64
  15. Kirimura K, Gunji H, Wakayama R, Hattori T, Ishii Y. 2010. Enzymatic Kolbe-Schmitt reaction to form salicylic acid from phenol: enzymatic characterization and gene identification of a novel enzyme, Trichosporon moniliiforme salicylic acid decarboxylase. Biochem Biophys Res Commun 2010;394:279-84. https://doi.org/10.1016/j.bbrc.2010.02.154
  16. Botes AL, Lotter J, Rhode OH, Botha A. 2005. Interspecies differences in the enantioselectivity of epoxide hydrolases in Cryptococcus laurentii (Kufferath) C.E. Skinner and Cryptococcus podzolicus (Bab'jeva & Reshetova) Golubev. Syst Appl Microbiol 2005;28:27-33. https://doi.org/10.1016/j.syapm.2004.10.003
  17. Ueda-Nishimura K, Mikata K. A new yeast genus, Tetrapisispora gen. nov.: Tetrapisispora iriomotensis sp. nov., Tetrapisispora nanseiensis sp. nov. and Tetrapisispora arboricola sp. nov., from the Nansei Islands, and reclassification of Kluyveromyces phaffii (van der Walt) van der Walt as Tetrapisispora phaffii comb. nov. Int J Syst Bacteriol 1999;49:1915-24. https://doi.org/10.1099/00207713-49-4-1915
  18. Ko PY, Seok SJ, Lee HB, Ko HS, Jeun YC. Species diversity of spontaneous mushrooms on Jeju Island. Kor J Mycol 2014;42:104-32. https://doi.org/10.4489/KJM.2014.42.2.104