The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Study of Tannin Reducing Effect of Aronia by Yeast Isolated from Jeotgal

젓갈에서 분리된 효모를 이용한 아로니아의 탄닌 성분 저감화 효과에 관한 연구

  • Received : 2015.11.27
  • Accepted : 2015.12.14
  • Published : 2015.12.31
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Abstract

Aronia (Black chokeberry, Aronia melanocarpa) belonging to the Rosaceae family, is native to eastern North America. Aronia contain high levels of flavonoids, mostly anthocyanins and proanthocyanidins, which are known as condensed tannins. The dominant proanthocyanidins in aronia are (-)-epicatechin and (+)-catechin. The concentration of proanthocyanidins in aronia is higher than in other berries, however due to the astringent taste it is not desirable for consumption. Therefore, the purpose of this study is to evaluate the effect of aronia on the reduction in tannins by yeast isolated from regional Jeotgal. We isolated strains of yeast with high ${\beta}$-glucosidase activity from Jeotgal, with the MTY2 strains exhibiting a reduction in final tannin concentration according to thin layer chromatography (TLC) analysis. MTY2 was confirmed as Kazachstania servazzii using an 18S rDNA sequence and named as K. servazzii MTY2. K. servazzii MTY2 showed most significant growth when K. servazzii MTY2 was cultured in a solution of 10% (w/v) glucose, 3% (w/v) tryptone and 0.1% (w/v) sodium chloride. According to the high performance liquid chromatography (HPLC) analysis, the (+) - catechin peak is present, but (-) - epicatechin peak was reduced at culture condition added with 10% glucose in medium.

젓갈에서 분리한 효모를 이용하여 아로니아의 탄닌 성분 저감화에 대해 조사하였다. TLC 실험을 통하여 최종 선별된 MTY2 균주는 18S rDNA sequence에 의해 Kazachstania servazzii으로 동정되어 K. servazzii MTY2로 명명하였다. K. servazzii MTY2의 생육은 배양 3시간 이후부터 급격하게 증가하였으며, 9시간에 최대 증식을 나타내었다. 또한, 균체가 증식됨에 따라 pH는 6에서 5까지 감소하였다. 5% 아로니아 추출물을 첨가했을 때 K. servazzii MTY2의 최적 발효조건을 실험한 결과, 탄소원 경우 glucose 10% (w/v) 첨가 시 균체의 생육이 가장 높았으며, 10% (w/v) glucose 고정으로 질소원 실험 결과 3% (w/v) tryptone 첨가 시 균체 생육이 높았다. 마지막으로 glucose 10% (w/v)와 tryptone 3% (w/v)를 고정으로 하고 무기염류 실험 결과 sodium chloride 0.1% (w/v)를 첨가했을 때 가장 높은 균체 생육을 보였다. 또한 탄소원, 질소원, 무기염류 각각의 균체 생장이 가장 잘된 배양액을 수포화-n-butanol로 추출, 농축하여 HPLC 분석을 한 결과 (+)-catechin peak는 모든 조건에서 꾸준히 존재하였지만, (-)-epicatechin peak는 glucose 10% (w/v) 첨가의 조건으로 배양할 시 검출되지 않았다. 따라서, K. servazzii MTY2을 glucose 10% (w/v) 첨가의 조건으로 아로니아와 함께 발효시킬 경우, 탄닌 성분의 저감화가 가능할 것으로 판단하였다. 따라서 탄닌 성분의 저감화를 통해 특유의 떫은맛을 감소시킨 아로니아를 식용 혹은 식품의 2차 가공물의 재료로 용이하게 이용할 수 있을 것으로 생각한다.

Keywords

References

  1. Jeppsson N. The effects of fertilizer rate on vegetative growth, yield and fruit quality, with special respect to pigments, in black chokeberry (Aronia melanocarpa) cv. 'Viking'. Sci Hortic (Amsterdam) 2000;83:127-37. https://doi.org/10.1016/S0304-4238(99)00070-9
  2. Jeppsson N. Genetic variation and fruit quality in sea buckthorn and black chokeberry [dissertation]. Uppsala: Swedish University of Agricultural Sciences; 1999.
  3. Hardin JW. The enigmatic chokeberries (Aronia, Rosaceae). Bull Torrey Bot Club 1973;100:178-84. https://doi.org/10.2307/2484630
  4. Kokotkiewicz A, Jaremicz Z, Luczkiewicz M. Aronia plants: a review of traditional use, biological activities, and perspectives for modern medicine. J Med Food 2010;13:255-69. https://doi.org/10.1089/jmf.2009.0062
  5. Oszmianski J, Wojdylo A. Aronia melanocarpa phenolics and their antioxidant activity. Eur Food Res Technol 2005;221:809-13. https://doi.org/10.1007/s00217-005-0002-5
  6. Slimestad R, Torskangerpoll K, Nateland HS, Johannessen T, Giske NH. Flavonoids from black chokeberries, Aronia melanocarpa. J Food Compost Anal 2005;18:61-8. https://doi.org/10.1016/j.jfca.2003.12.003
  7. Lila MA. From beans to berries and beyond: teamwork between plant chemicals for protection of optimal human health. Ann N Y Acad Sci 2007;1114:372-80. https://doi.org/10.1196/annals.1396.047
  8. Santos-Buelga C, Scalbert A. Proanthocyanidins and tanninlike compounds-nature, occurrence, dietary intake and effects on nutrition and health. J Sci Food Agric 2000;80:1094-117. https://doi.org/10.1002/(SICI)1097-0010(20000515)80:7<1094::AID-JSFA569>3.0.CO;2-1
  9. Evans WC. Trease and Evans' pharmacognosy. 13th ed. London: Bailliere Tindall; 1989.
  10. Bate-Smith EC, Swain T. Flavonoid compounds. In: Florkin M, Mason HS, editors. Comparative biochemistry. New York: Academic Press; 1962. p. 705-809.
  11. Haslam E. Plant polyphenols: vegetable tannins revisited. Cambridge: Cambridge University Press; 1989.
  12. Oszmianski J, Kucharska A. Tannins aronia. Scientific Papers of the Agricultural University of Wroclaw. Food Technol 1995;8:55-65.
  13. Gu L, Kelm MA, Hammerstone JF, Beecher G, Holden J, Haytowitz D, Gebhardt S, Prior RL. Concentrations of proanthocyanidins in common foods and estimations of normal consumption. J Nutr 2004;134:613-7. https://doi.org/10.1093/jn/134.3.613
  14. Skupien K, Oszmianski J. The effect of mineral fertilization on nutritive value and biological activity of chokeberry fruit. Agric Food Sci 2007;16:46-55. https://doi.org/10.2137/145960607781635822
  15. Seo JH, Jeong YJ, Shin SR, Kim KS. Effects of tannins from astringent persimmons in alcohol fermentation for persimmon vinegars. J Korean Soc Food Sci Nutr 2000;29:407-11.
  16. Atlas RM, Parks LC. Handbook of microbiological media. Boca Raton: CRC Press; 1993.
  17. Amarowicz R, Kozlowska H, Shimoyamada M, Okubo K. Chromatographic analysis of rapeseed glucoside fractions. Pol J Food Nutr Sci 1992;1:89-93.
  18. Lee S, Lee YH, Park JM, Bai DH, Jang JK, Park YS. Bioconversion of ginsenosides from red ginseng extract using Candida allociferrii JNO301 isolated from Meju. Mycobiology 2014;42:368-75. https://doi.org/10.5941/MYCO.2014.42.4.368
  19. Lee YH. Bioconversion of ginsenosides by Candida allociferrii JNO301 isolated from Meju [dissertation]. Seongnam: Kyungwon University; 2011.