Mycobiology

The Korean Society of Mycology (한국균학회)
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The Effects of Different Substrates on the Growth, Yield, and Nutritional Composition of Two Oyster Mushrooms (Pleurotus ostreatus and Pleurotus cystidiosus)

  • Hoa, Ha Thi (Department of Tropical Agriculture and International Cooperation, National Pingtung University of Science and Technology) ;
  • Wang, Chun-Li (Department of Plant Industry, National Pingtung University of Science and Technology) ;
  • Wang, Chong-Ho (Department of Plant Industry, National Pingtung University of Science and Technology)
  • Received : 2015.06.07
  • Accepted : 2015.09.29
  • Published : 2015.12.31
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Abstract

The study was conducted to compare the effects of different agro-wastes on the growth, yield, and nutritional composition of oyster mushrooms Pleurotus ostreatus (PO) and Pleurotus cystidiosus (PC). Seven substrate formulas including sawdust (SD), corncob (CC), sugarcane bagasse (SB) alone and in combination of 80 : 20, 50 : 50 ratio between SD and CC, SD and SB were investigated. The results indicated that different substrate formulas gave a significant difference in total colonization period, characteristics of fruiting bodies, yield, biological efficiency (BE), nutritional composition and mineral contents of two oyster mushrooms PO and PC. The results showed that increasing CC and SB reduced C/N ratio, and enhanced some mineral contents (Ca, P, and Mg) of substrate formulas. The increased amount of CC and SB of substrate formulas enhanced protein, ash, mineral contents (Ca, K, Mg, Mn, and Zn) of fruiting bodies of both mushrooms. Substrates with 100% CC and 100% SB were the most suitable substrate formulas for cultivation of oyster mushrooms PO and PC in which they gave the highest values of cap diameter, stipe thickness, mushroom weight, yield, BE, protein, fiber, ash, mineral content (Ca, K, and Mg) and short stipe length. However, substrate formula 100% CC gave the slowest time for the first harvest of both mushrooms PO and PC (46.02 days and 64.24 days, respectively). It is also found that the C/N ratio of substrate formulas has close correlation with total colonization period, mushroom weight, yield, BE and protein content of mushroom PO and PC.

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References

  1. Sanchez C. Cultivation of Pleurotus ostreatus and other edible mushrooms. Appl Microbiol Biotechnol 2010;85:1321-37. https://doi.org/10.1007/s00253-009-2343-7
  2. Kues U, Liu Y. Fruiting body production in Basidiomycetes. Appl Microbiol Biotechnol 2000;54:141-52. https://doi.org/10.1007/s002530000396
  3. Obodai M, Cleland-Okine J, Vowotor KA. Comparative study on the growth and yield of Pleurotus ostreatus mushroom on different lignocellulosic by-products. J Ind Microbiol Biotechnol 2003;30:146-9. https://doi.org/10.1007/s10295-002-0021-1
  4. Mane VP, Patil SS, Syed AA, Baig MM. Bioconversion of low quality lignocellulosic agricultural waste into edible protein by Pleurotus sajor-caju (Fr.) Singer. J Zhejiang Univ Sci B 2007;8:745-51. https://doi.org/10.1631/jzus.2007.B0745
  5. Garcha H, Khanna P, Soni G. Nutritionla importance of mushrooms. In: Chang ST, Buswell JA, Chiu SW, editors. Mushroom biology and mushroom products. Hong Kong: Chinese University Press; 1993. p. 227-36.
  6. Tesfaw A, Tadesse A, Kiros G. Optimization of oyster (Pleurotus ostreatus) mushroom cultivation using locally available substrates and materials in Debre Berhan, Ethiopia. J Appl Biol Biotechnol 2015;3:15-20.
  7. Baysal E, Peker H, Yalinkilic MK, Temiz A. Cultivation of oyster mushroom on waste paper with some added supplementary materials. Bioresour Technol 2003;89:95-7. https://doi.org/10.1016/S0960-8524(03)00028-2
  8. Chang ST, Miles PG. Edible mushroom and their cultivation. Boca Raton (FL): CRC Press; 1989.
  9. Badu M, Twumasi SK, Boadi NO. Effect of lignocellulosic in wood used as substrate on the quality and yield of mushrooms. Food Nutr Sci 2011;2:780-4. https://doi.org/10.4236/fns.2011.27107
  10. Patil SS, Ahmed SA, Telang SM, Baig MM. The nutritional value of Pleurotus ostreatus (Jacq.:Fr) Kumm cultivated on different lignocellulosic agro-wastes. Innov Rom Food Biotechnol 2010;7:66-76.
  11. Szabova E, Rohalova L, Hedvigy M. Semi-solid fermentation of Pleurotus ostreatus. J Microbiol Biotechnol Food Sci 2013; 2:1950-8.
  12. Sivrikaya H, Bacak L, Saracbasi A, Toroglu I, Eroglu H. Trace elements in Pleurotus sajor-caju cultivated on chemithermomechanical pulp for bio-bleaching. Food Chem 2002;79:173-6. https://doi.org/10.1016/S0308-8146(02)00128-0
  13. Chang ST. Mushroom cultivation using the "ZERI" principle: potential for application in Brazil. Micol Aplicada Int 2007; 19:33-4.
  14. Li L, Ng TB, Song M, Yuan F, Liu ZK, Wang CL, Jiang Y, Fu M, Liu F. A polysaccharide-peptide complex from abalone mushroom (Pleurotus abalonus) fruiting bodies increases activities and gene expression of antioxidant enzymes and reduces lipid peroxidation in senescence-accelerated mice. Appl Microbiol Biotechnol 2007;75:863-9. https://doi.org/10.1007/s00253-007-0865-4
  15. Chorvathoba V, Bobek P, Ginter E, Klvanova J. Effect of the oyster fungus on glycaemia and cholesterolaemia in rats with insulin-dependent diabetes. Physiol Res 1993;42:175-9.
  16. Rizki M, Tamai Y. Effects of different nitrogen rich substrates and their combination to the yield performance of oyster mushroom (Pleurotus ostreatus). World J Microbiol Biotechnol 2011;27:1695-702. https://doi.org/10.1007/s11274-010-0624-z
  17. Nelson DW, Sommers LE. Total carbon, organic carbon, and organic matter. In: Page AL, Miller RH, Keeney DR, editors. Methods of soil analysis. Part 2. Chemical and microbiological properties. Agronomy monograph 9. Madison (WI): American Society of Agronomy; 1982. p. 539-79.
  18. Ezeibekwe IO, Ogbonnaya CI, Unamba CI, Osuala OM. Proximate analysis and mineral composition of edible mushrooms in parts of South Eastern Nigeria. Rep Opin 2009;1:32-6.
  19. Cavins TJ, Whipker BE, Fonteno WC, Harden B, McCall I, Gibson JL. Monitoring and managing pH and EC using the PourThru extraction method. Horticulture Information Leaflet 590. Raleigh (NC): North Carolina State University; 2000.
  20. Association of Official Annalytical Chemists. Official methods of analysis of AOAC International. 16th ed. Washington, DC: Association of Official Annalytical Chemists; 1995.
  21. Oei P. Small-scale mushroom cultivation: oyster, shiitake and wood ear mushrooms. First edition of Digigrafi, Wageningen, The Netherlands. Wageningen: Agromisa Foundation and CTA; 2005.
  22. Sales-Campos C, Fereira da Eira A, Teixeira de Almeida Minhoni M, Nogueira de Andrade MC. Mineral composition of raw material, substrate and fruiting bodies of Pleurotus ostreatus in culture. Interciencia 2009;34:432-6.
  23. Albores S, Pianzzola MJ, Soubes M, Cerdeiras MP. Biodegradation of agroindustrial wastes by Pleurotus spp for its use as ruminant feed. Electron J Biotechnol 2006;9:215-20.
  24. Naraian R, Sahu RK, Kumar S, Garg SK, Singh CS, Kanaujia RS. Influence of different nitrogen rich supplements during cultivation of Pleurotus florida on corn cob substrate. Environmentalist 2009;29:1-7. https://doi.org/10.1007/s10669-008-9174-4
  25. Yang XM. Cultivation of edible mushroom. Beijing: China Agriculture Press; 2000.
  26. Dahmardeh M, Dahmardeh M, Hossienabadi R, Safarpoor H, Dahmardeh M. Comparative study on cultivation and yield performance of Pleurotus ostreatus (oyster mushroom) grown on different substrates (wheat straw and barley straw) and supplemented at various levels of spawn. J Food Agric Environ 2010;8:996-8.
  27. Bughio I. Yelid performance of oyster mushroom, Pleurotus ostreatus (Jacq. ex. Fr.) Kummer on combination of different straws [dissertation]. Tando Jam: Sindh Agriculture University; 2001.
  28. Bugarski D, Gvozdenovic D, Takac A, Cervenski J. Yield and yield components of different strains of oyster mushroom. Savremena Poljoprivreda (Yugoslavia) 1994;42:314-8.
  29. Mondal SR, Rehana J, Noman MS, Adhikary SK. Comparative study on growth and yield performance of oyster mushroom (Pleurotus florida) on different substrates. J Bangladesh Agric Univ 2010;8:213-20.
  30. Bhattacharjya DK, Paul RK, Miah MN, Ahmed KU. Effect of different saw dust substrates on the growth and yield of oyster mushroom (Pleurotus ostreatus). J Agric Vet Sci 2014; 7:38-46.
  31. Das N, Mukherjee M. Cultivation of Pleurotus ostreatus on weed plants. Bioresour Technol 2007;98:2723-6. https://doi.org/10.1016/j.biortech.2006.09.061
  32. Yang WJ, Guo FL, Wan ZJ. Yield and size of oyster mushroom grown on rice/wheat straw basal substrate supplemented with cotton seed hull. Saudi J Biol Sci 2013;20:333-8. https://doi.org/10.1016/j.sjbs.2013.02.006
  33. Liang ZC, Wu KJ, Wang JC, Lin CH, Wu CY. Cultivation of the culinary-medicinal lung oyster mushroom, Pleurotus pulmonarius (Fr.) Quel. (Agaricomycetideae) on grass plants in Taiwan. Int J Med Mushrooms 2011;13:193-9. https://doi.org/10.1615/IntJMedMushr.v13.i2.120
  34. Serra MD, Kirby R. Development of Pleurotus (oyster) mushroom production in South Africa using alien wood species as lignocellulose substrate. Int J Mushroom Sci 1999; 2:49-55.
  35. Mintesnot B, Ayalew A, Kebede A. Evaluation of biomass of some invasive weed species as substrate for oyster mushroom (Pleurotus spp.) cultivation. Pak J Biol Sci 2014;17:213-9. https://doi.org/10.3923/pjbs.2014.213.219
  36. Ashrafi R, Mian MH, Rahman MM, Jahiruddin M. Recycling of spent mushroom substrate for the production of oyster mushroom. Res Biotechnol 2014;5:13-21.
  37. Chang ST, Miles PG. A new look at cultivated mushroom. BioScience 1984;34:358-62. https://doi.org/10.2307/1309726
  38. Taurachand D. Sugarcane bagasse. In: Choi KW, editor. Mushroom growers' handbook 1. Oyster mushroom cultivation. Seoul: MushWorld; 2004. p. 118-21.
  39. Philippoussis A, Diamantopoulou P, Israilides C. Productivity of agricultural residues used for the cultivation of the medicinal fungus Lentinula edodes. Int Biodeterior Biodegradation 2007;59:216-9. https://doi.org/10.1016/j.ibiod.2006.10.007
  40. Wang D, Sakoda A, Suzuki M. Biological efficiency and nutritional value of Pleurotus ostreatus cultivated on spent beer grain. Bioresour Technol 2001;78:293-300. https://doi.org/10.1016/S0960-8524(01)00002-5
  41. Philippoussis AN, Diamantopoulou PA, Zervakis GI. Correlation of the properties of several lignocellolosic substrates to the crop performance of shitake mushroom Lentinula edodes. World J Microbiol Biotechnol 2003;19:551-7. https://doi.org/10.1023/A:1025100731410
  42. Quimio TH. Introducing: Pleurotus flabellatus for your dinner table. Mushroom J 1978;69:282-3.
  43. Sivaprakasam K. Studies on oyster mushroom (Pleurotus sajorcaju) Fr. [dissertation]. Coimbatore: Tamil Nadu Agricultural University; 1980.
  44. Kurtzman RH. A review mushrooms: sources for modern Western medicine. Micol Aplicada Int 2005;17:21-33.
  45. Ahmed SA, Kadam JA, Mane VP, Patil SS, Baig MM. Biological effeciency and nutritional contents of Pleurotus florida (Mont.) Singer cultivated on different agro-wastes. Nat Sci 2009;7:44-8.
  46. Ragunathan R, Swaminathan K. Nutritional status of Pleurotus spp. grown on various agro-wastes. Food Chem 2003;80:371-45. https://doi.org/10.1016/S0308-8146(02)00275-3
  47. Mshandete AM, Cuff J. Proximate and nutrient composition of three types of indigenous edible wild mushroom grown in Tanzania and their utilization prospects. Afr J Food Agric Nutr Dev 2007;7:1-16.
  48. Sharma S, Yadav RK, Pokhrel CP. Growth and yield of oyster mushroom (Pleurotus ostreatus) on different substrates. J New Biol Rep 2013;2:3-8.
  49. Bonatti-Chaves M, Karnopp P, Soares HM, Furlan SA. Evaluation of Pleurotus ostreatus and P. sajor-caju nutritional characteristics when cultivated in different lignocellulosic wastes. Food Chem 2004;88:425-8. https://doi.org/10.1016/j.foodchem.2004.01.050
  50. Khan MA, Amin SM, Uddin MN, Tania M, Alam N. Comparative study of the nutritional composition of oyster mushrooms cultivated in Bangladesh. Bangladesh J Mushroom 2008;2:9-14.
  51. Kalac P, Svoboda L. A review of trace element concentrations in edible mushrooms. Food Chem 2000;69:273-81. https://doi.org/10.1016/S0308-8146(99)00264-2