Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
권호 표지
DOI QR코드

DOI QR Code

Antibacterial activity of isothiocyanates from cruciferous vegetables against pathogenic bacteria in olive flounder

십자화과 채소 유래 isothiocyanates의 넙치 어병세균에 대한 항균활성

  • Ko, Mi-Ok (Department of Food Bioengineering, Jeju National University) ;
  • Ko, Jeong-Yeon (Department of Food Bioengineering, Jeju National University) ;
  • Kim, Mi-Bo (Jeju Wellbeing Vegetables RIS System, Jeju National University) ;
  • Lim, Sang-Bin (Department of Food Bioengineering, Jeju National University)
  • 고미옥 (제주대학교 식품생명공학과) ;
  • 고정연 (제주대학교 식품생명공학과) ;
  • 김미보 (제주대학교 제주양채류RIS사업단) ;
  • 임상빈 (제주대학교 식품생명공학과)
  • Received : 2015.10.30
  • Accepted : 2015.11.09
  • Published : 2015.12.30
Download PDF
⟨ Previous Next ⟩

Abstract

The antimicrobial effects of ten isothiocyanates (ITCs) present in cruciferous vegetables and radish root hydrolysate were investigated against pathogenic bacteria from olive flounder. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were measured against two gram-positive bacterial strains (Streptococcus parauberis, S. iniae) and four gram-negative bacterial strains (Edwardsiella tarda, Vibrio ichthyoenteri, V. harveyi, Photobacterium damselae) by using a broth microdilution technique. The antibacterial activity of ITCs was in the order sulforaphane > sulforaphene > phenylethyl ITC > erucin > benzyl ITC > iberin > I3C > allyl ITC > phenyl ITC > hexyl ITC. The susceptibility of fish pathogens to ITCs was in the order of V. harveyi > E. tarda > P. damselae > S. parauberis > S. iniae > V. ichthyoenteri. Antimicrobial activity (MIC) of radish root hydrolysate was 0.250 mg/mL against S. iniae, 0.438 mg/mL against S. parauberis, and 0.500 mg/mL against both E. tarda and V. harveyi. The aliphatic ITCs were potent inhibitors of the growth of fish pathogens, followed by aromatic ITCs and indolyl ITC. The presence of a double bond in the chemical structure of ITCs decreased antibacterial activity, while ITCs with a thiol (-S-) group and a longer carbon chain increased antibacterial activity. These results suggest that ITCs have strong antibacterial activities and may be useful in the prevention of fish pathogens.

십자화과 채소의 주요 성분인 10종의 isothiocyanates(ITCs)와 무 가수분해물을 대상으로 6개의 넙치 어병세균에 대하여 항균활성을 측정하여 ITCs의 화학적 구조와 항균성과의 관계를 비교하였다. 항균활성은 sulforaphane, sulforaphene, PEITC, erucin, BITC, iberin, I3C가 높았으며, AITC, PITC, HITC는 낮았다. 어병 세균별로 ITCs에 대한 민감성은V. harveyi가 가장 높았으며, 그 다음으로E. tarda, P. damselae, S. parauberis, S. iniae, V. ichthyoenteri 순으로, 그람음성균이 그람양성균에 비하여 민감성이 높았다. 무가수분해물의 최소저해농도(MIC)는 S. iniae에 대해서 0.250 mg/mL(raphasatin의 농도)로 가장 높은 항균활성을 나타내었고, S. parauberis는 0.438 mg/mL, E. tarda와 V. harveyi는 0.500 mg/mL로 높은 항균활성을 나타내었다. ITCs의 화학구조에 따른 어병 세균에 대한 항균활성은 aliphatic ITCs 중에서는 sulforaphene, sulforaphane, erucin, iberin의 항균활성이 높았으며, benzene ring을 함유하고 있는 aromatic ITCs 중에서는 PEITC과 BITC이 가장 항균활성이 높았다. 이중결합이 없는 sulforaphane은 이중결합을 가지고 있는 sulforaphene에 비하여 항균활성이 대부분의 균주에 대해서 높았다. Thiol group을 가지고 있는 erucin은 sulfinyl group을 가지고 있는 sulforaphane에 비하여 일부균주에 대하여 높은 항균활성을 나타내었다. 탄소사슬의 길이가 긴 PEITC는 탄소사슬의 길이가 짧은 BITC에 비하여 4가지 균주에 대하여 항균활성이 높았다. 이상의 결과로 부터 십자화과 유래 ITCs는 넙치 어병 항균제로 활용할 수 있을 것으로 추정되었다.

Keywords

References

  1. Wennberg M, Ekvall J, Olsson K, Nyman M (2006) Changes in carbohydrate and glucosinolate composition in white cabbage (Brassica oleracea var. capitata) during blanching and treatment with acetic acid. Food Chem, 95, 226-236 https://doi.org/10.1016/j.foodchem.2004.11.057
  2. Clarke DB (2010) Glucosinolates, structures and analysis in food. Anal Methods, 2, 310-325 https://doi.org/10.1039/b9ay00280d
  3. Agerbirk N, Olsen CE (2012) Glucosinolate structures in evolution. Phytochem, 77, 16-45 https://doi.org/10.1016/j.phytochem.2012.02.005
  4. Bones AM, Rossiter JT (1996) The myrosinase-glucosinolate system, its organisation and biochemistry. Physiol Plant, 97, 194-208 https://doi.org/10.1111/j.1399-3054.1996.tb00497.x
  5. Dias C, Aires A, Saavedra MJ (2014) Antimicrobial activity of isothiocyanates from cruciferous plants against methicillin-resistant Staphylococcus aureus (MRSA). Int J Mol Sci, 15, 19552-19561 https://doi.org/10.3390/ijms151119552
  6. Shen L, Su G, Wang X, Du Q, Wang K (2010) Endogenous and exogenous enzymolysis of vegetable-sourced glucosinolates and influencing factors. Food Chem, 119, 987-994 https://doi.org/10.1016/j.foodchem.2009.08.003
  7. Yuan G, Wang X, Guo R, Wang Q (2010) Effect of salt stress on phenolic compounds, glucosinolates, myrosinase and antioxidant activity in radish sprouts. Food Chem, 121, 1014-1019 https://doi.org/10.1016/j.foodchem.2010.01.040
  8. Pocasap P, Weerapreeyakul N, Barusrux S (2013) Cancer preventive effect of Thai rat-tailed radish (Raphanus sativus L. var. caudatus Alef). J Funct Foods, 5, 1372-1381 https://doi.org/10.1016/j.jff.2013.05.005
  9. Fahey JW, Zalcmann AT, Talalay P (2001) The chemical diversity and distribution of glucosinolates and isothiocyanates among plants. Phytochem, 56, 5-51 https://doi.org/10.1016/S0031-9422(00)00316-2
  10. Dufour V, Stahl M, Baysse C (2015) The antibacterial properties of isothiocyanates. Microbiol, 161, 229-243 https://doi.org/10.1099/mic.0.082362-0
  11. Ahn ES, Kim JH, Shin DH (1999) Antimicrobial effects of allyl isothiocyanates on several microorganisms. Korean J Food Sci Technol, 31, 206-211
  12. Wilson AE, Bergaentzle M, Bindler F, Marchioni E (2013) In vitro efficacies of various isothiocyanates from cruciferous vegetables as antimicrobial agents against foodborne pathogens and spoilage bacteria. Food Control, 30, 318-324 https://doi.org/10.1016/j.foodcont.2012.07.031
  13. Kang BJ (2003) A study on the characteristics of bacteria isolated from cultured flounders (Paralichthys olivaceus) showing disease symptoms in Jeju area of Korea. Ph D Thesis. Jeju National University, Korea, p 1-112
  14. Choi HS, Kim YC, Lee JS, Jo MR, Seo CH, Park SI (2004) Antibacterial activities of hot-water and ethyl alcohol extracts of medicinal herbs of fish pathogenic bacteria. J Fish Pathol, 17, 39-55
  15. Moon YG, Choi KS, Lee KJ, Kim KY, Heo MS (2006) Screening of antioxidative and antibacterial activity from hot water extracts of indigenous plants, Jeju-island. Korean J Biotechnol Bioeng, 21, 164-169
  16. Choi HS, Kim JS, Jang DS, Yu YB, Kim YC, Lee JS (2005) Antibacterial activities of Galla rhois extracts against fish pathogenic bacteria. J Fish Pathol, 18, 239-245
  17. Kim KH, Kim AR, Cho EJ, Joo SJ, Park JH, Moon JY, Yum JH, Kim TH, Kwon HJ, Lee HT, Kim YM, Lee EW (2014) Antibacterial activity of Rhus javanica against the fish pathogens Vibrio ichthyoenteri and Streptococcus iniae. Korean J Fish Aquat Sci, 47, 018-022 https://doi.org/10.5657/KFAS.2014.0018
  18. Kim SM, Jun LJ, Yeo IK, Jeon YJ, Lee KJ, Jeon HD, Jeong JB (2014) Effects of dietary supplementation with garlic extract on immune responses and diseases resistance of olive flounder, Paralichthys olivaceus. J Fish Pathol, 27, 35-45 https://doi.org/10.7847/jfp.2014.27.1.035
  19. Kim JW, Kim MB, Lim SB (2015) Formation and stabilization of raphasatin and sulforaphene from radish roots by endogenous enzymolysis. Prev Nutr Food Sci, 20, 119-125 https://doi.org/10.3746/pnf.2015.20.2.119
  20. CLSI (2012) Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; Approved Standard. 9th ed, Document M07-A9, Clinical and Laboratory Standards Institute, Wayne PA, 1-63
  21. Kim MS, Cho JY, Seo JS, Jung SH, Choi HS, Park MA (2012) Distribution of MIC value of antibiotics against Edwardsiella tarda isolated from olive flounder (Paralichthys olivaceus). J Fish Pathol, 25, 181-188 https://doi.org/10.7847/jfp.2012.25.3.181
  22. Bulfon C, Volpatti D, Galeotti M (2014) In vitro antibacterial activity of plant ethanolic extracts against fish pathogens. J World Aquacult Soc, 45, 545-557 https://doi.org/10.1111/jwas.12151
  23. Scholl C, Eshelman BD, Barnes DM, Hanlon PR (2011) Raphasatin is a more potent inducer of the detoxification enzymes than its degradation products. J Food Sci, 76, C504-C511
  24. Han SM, Lee KG, Park KK (2011) Antimicrobial activiy of honeybee venom against fish pathogenic bacteria. J Fish Pathol, 24, 113-120 https://doi.org/10.7847/jfp.2011.24.2.113
  25. Kang MA, Lim MB, Kim JH, Ko YH, Lim SB (2010) Integral antioxidative capacity and antimicrobial activity of pressurized liquid extracts from 40 selected plant species. J Korean Soc Food Sci Nutr, 39, 1249-1256 https://doi.org/10.3746/jkfn.2010.39.9.1249