Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
권호 표지
DOI QR코드

DOI QR Code

Analysis of γ-Aminobutyric Acid Content in Fermented Plant Products by HPLC/UV

  • Lee, Dong Gu (Department of Integrative Plant Science, Chung-Ang University) ;
  • Cho, Sunghun (Department of Integrative Plant Science, Chung-Ang University) ;
  • Lee, Jamin (Department of Integrative Plant Science, Chung-Ang University) ;
  • Cho, Seon Haeng (Gongju National University of Education) ;
  • Lee, Sanghyun (Department of Integrative Plant Science, Chung-Ang University)
  • Received : 2015.06.12
  • Accepted : 2015.08.21
  • Published : 2015.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

${\gamma}$-Aminobutyric acid (GABA) content in fermented plant products and their main plant materials (aerial part of Acanthopanax sessiliflorus, fruit of Crataegus pinnatifida, and whole plant of Morus alba) was determined by high-performance liquid chromatography. GABA was quantified using a reverse-phase column with a gradient elution program (water:acetonitrile =90:10 to 0:100 for 40 min). UV detection was conducted at 280 nm. GABA content was measured in fermented plant products (15.07 mg/g), aerial part of A. sessiliflorus (4.49 mg/g), fruit of C. pinnatifida (10.59 mg/g), and whole plant of M. alba (2.31 mg/g). The presence of GABA in fermented plant products, including A. sessiliflorus, C. pinnatifida, and M. alba is important in industrial application for health supplements.

Keywords

References

  1. Abdou AM, Higashiguchi S, Horie K, Kim M, Hatta H, and Yokogoshi H (2006) Relaxation and immunity enhancement effects of gammaaminobutyric acid (GABA) administration in humans. Biofactors 26, 201-8. https://doi.org/10.1002/biof.5520260305
  2. Agarwal S and Kanwar K (2007) Comparison of genetic transformation in Morus alba L. via different regeneration systems. Plant Cell Rep 26, 177-85. https://doi.org/10.1007/s00299-006-0217-3
  3. Ahn KS, Hahm MS, Park EJ, Lee HK, and Kim IH (1998) Crosolic acid isolated from fruit Crataegus pinnatifida var. pilosa is protein kinase C inhibitor as well as a cytotoxic agent. Planta Med 64, 468-70. https://doi.org/10.1055/s-2006-957487
  4. Al Makdessi S, Sweidan H, Mullner S, and Jocob R (1996) Myocardial protection by pretreatment with Crataegus oxycantha: An assessment by means of the release of lactate dehydrogenase by the ischemic and reperfused Langendorff heart. Arzneimitt Forsch 46, 25-7.
  5. An Q, Yang CJ, Song Y, Yu K, Xiong ZL, and Li FM (2008) Studies on the chemical constituents of the fruit of Acanthopanax sessiliflorus (Rupr. et Maxim.) Seem. Nat Prod Res Dev 20, 765-9.
  6. Aoki H, Furuya Y, Endo Y, and Fujimoto K (2003) Effect of $\gamma$-aminobutyric acid-enriched tempeh-like fermented soybean (GABA-tempeh) on the blood pressure of spontaneously hypertensive rats. Biosci Biotechnol Biochem 67, 1806-8. https://doi.org/10.1271/bbb.67.1806
  7. Brekhman II and Dardymov IV (1969) Substances of plant origin which increase nonspecific resistance. Annu Rev Pharmacol 9, 415.
  8. Du J, He ZD, Jiang RW, Ye WC, Xu HX, and But PPH (2003) Antiviral flavonoids from the root bark of Morus alba L. Phytochemistry 62, 1235-8. https://doi.org/10.1016/S0031-9422(02)00753-7
  9. Edwards JE, Brown PN, Talent N, Dickinson TA, and Shipley PR (2012) A review of the chemistry of the genus Crataegus. Phytochemistry 79, 5-26. https://doi.org/10.1016/j.phytochem.2012.04.006
  10. Fong HHS and Bauman JL (2002) Hawthorn in cardiovascular disease. J Cardiovasc Nurs 16, 1-8.
  11. Hayakaw K, Kimura M, Kasaha K, Matsumoto K, Sansawa H, and Yamori Y (2004) Effect of a $\gamma$-aminobutyric acid-enriched dairy product on the blood pressure of spontaneously hypertensive and normotensive Wistar-Kyoto rats. British J Nutr 92, 411-7. https://doi.org/10.1079/BJN20041221
  12. Huang LZ, Huang BK, Ye Q, and Qin LP (2011) Bioactivity-guided fractionation for anti-fatigue property of Acanthopanax senticosus. J Ethnopharmacol 133, 213-9. https://doi.org/10.1016/j.jep.2010.09.032
  13. Huang XX, Guo DD, Li LZ, Lou LL, Li DM, Zhou CC et al. (2013) Monoterpene and sesquilignan compounds from the leaves of Crataegus pinnatifida. J Biochem. Syst Ecol 48, 1-5. https://doi.org/10.1016/j.bse.2012.12.001
  14. Inoue K, Shirai T, Ochiai H, Kasao M, Hayakawa K, Kimura M et al. (2003) Blood-pressure-lowering effect of a novel fermented milk containing $\gamma$- aminobutyric acid (GABA) in mild hypertensives. Eur J Clin Nutr 57, 490-5. https://doi.org/10.1038/sj.ejcn.1601555
  15. Jakobs C, Jaeken J, and Gibson KM (1993) Inherited disorders of GABA metabolism. J Inh Metab Dis 16, 704-5. https://doi.org/10.1007/BF00711902
  16. Jin JL, Lee S, Lee YY, Kim JM, Heo JE, and Choi HS (2004) Platelet antiaggregating triterpenoids from the leaves of Acanthopanax senticosus and the fruits of A. sessiliflorus. Planta Med 70, 564-6. https://doi.org/10.1055/s-2004-827159
  17. Kim HY, Lee DG, Lee KH, and Lee S (2012) Protective effects of 3,4-secolupane type triterpenes from Acanthopanax senticosus against advanced glycation endproducts. Hort Environ Biotechnol 53, 242-6. https://doi.org/10.1007/s13580-012-0030-6
  18. Komassuzaki N, Shima J, Kawamoto S, Momose H, and Kimura T (2005) Production of $\gamma$-aminobutyric acid (GABA) by Lactobacillus paracasei isolated from traditional fermented foods. Food Microbiol 22, 497-504. https://doi.org/10.1016/j.fm.2005.01.002
  19. Lee SH, Lee YS, Jung SH, Ji J, Shin KH, Kim B-K et al. (2003) Antitumor and immunostimulating activities of Acanthopanax sessiliflorus fruits. Nat Prod Sci 9, 112-6.
  20. Lee WT (1979) Distribution Acanthopanax plants in Korea. Kor J Pharmacol 10, 103-7.
  21. Lin SH, Mau JL, and Hsu CA (2012) Bioactive components and antioxidants of tea leaves. Food Sci Technol 46, 64-70.
  22. Liu FZ, Ren JW, Tang JS, Liu XZ, Che YS, and Yao XS (2013) Cyclohexanone derivatives with cytotoxicity from the fungus Penicillium commune. Fitoterapia 87, 78-83. https://doi.org/10.1016/j.fitote.2013.03.022
  23. Manyam BV, Katz L, Hare TA, Kaniefski K, and Tremblay RD (1981) Isoniazidinduced elevation of cerebrospinal fluid (CSF) GABA levels and effects on chorea in huntington's disease. Ann Neurol 10, 35-7. https://doi.org/10.1002/ana.410100107
  24. Nam SY, Yi HK, Lee JC, Kim JC, Song CH, Park JW et al. (2002) Cortex mori extract induces cancer cell apoptosis through inhibition of microtubule assembly. Arch Pharm Res 25, 191-6. https://doi.org/10.1007/BF02976562
  25. Oh CH and Oh SH (2004) Effects of germinated brown rice extracts with enhanced levels of GABA on cancer cell proliferation and apoptosis. J Med Food 7, 19-23. https://doi.org/10.1089/109662004322984653
  26. Oh SH, Soh JR, and Cha YS (2003) Germinated brown rice extract shows a nutraceutical effect in the recovery of chronic alcohol-related symptoms. J Med Food 7, 115-21.
  27. Park KM, You JS, Lee HY, Baek NI, Hwang JK, and Kuwanon G (2003) An antibacterial agent from the root bark of Morus alba against oral pathogens. J Ethnopharmacol 84, 181-5. https://doi.org/10.1016/S0378-8741(02)00318-5
  28. Popping S, Rose H, Ioneseu I, Fisher Y, and Kammermeier H (1995) Effect of a hawthorn extract on contraction and energy turnover of isolated rat cardiomyocytes. Arzneimitt Forsch 11, 1157-61.
  29. Sendker J, Petereit F, Lautenschlager M, Hellenbrand N, and Hensel A (2013) Phenylpropanoid-substituted procyanidins and tentatively identified procyanidin glycosides from hawthorn (Crataegus spp.). Planta Med 79, 45-51.
  30. Shi YQ, Fukai T, Sakagami H, Chang WJ, Yang PQ, Wang FP et al. (2001) Cytotoxic flavonoids with isoprenoid groups from Morus mongolica. J Nat Prod 64,181-8. https://doi.org/10.1021/np000317c
  31. Shin KH and Lee S (2002) The chemistry of secondary products from Acanthopanax species and their pharmacological activities. Nat Prod Sci 8, 111-26.
  32. Shohael AM, Chakrabarty D, Yu KW, Hahn EJ, and Paek KY (2005) Application of bioreactor system for large-scale production of Eleutherococcus sessiliflorus somatic embryos in an air-lift bioreactor and production of eleutherosides. J Biotechnol 120, 228-36. https://doi.org/10.1016/j.jbiotec.2005.06.010
  33. Song SJ, Li LZ, Gao PY, Yuan YQ, Wang RP, Liu KC et al. (2012a) Isolation of antithrombotic phenolic compounds from the leaves of Crataegus pinnatifida. Planta Med 78, 1967-71. https://doi.org/10.1055/s-0032-1327877
  34. Song Y, Deng Y, Huang D, Wen J, Liu Z, and Li F (2012b) LC-MS/MS determination and pharmacokinetic study of four lignin components in rat plasma after oral administration of Acanthopanax sessiliflorus extract. J Ethnopharmacol 141, 957-63. https://doi.org/10.1016/j.jep.2012.03.034
  35. Song Y, Yang CJ, Yu K, and Li FM (2011) In vivo antithrombotic and antiplatelet activites of a quantified Acanthopanax sessiliflorus fruit extract. Chin J Nat Med 9, 0141-5.
  36. Tsushida T, Murai T, Omori M, and Okamoto J (1987) Production of new type tea containing a high level of $\gamma$-aminobutyric acid. Nippon Nogeikagaku Kaishi 61, 817-22. https://doi.org/10.1271/nogeikagaku1924.61.817
  37. Wang JJ, Wang HY, and Shih CD (2010) Autonomic nervous system and nitric oxide in antihypertensive and cardiac Inhibitory effects Induced by red mold rice in spontaneously hypertensive rats. J Agric Food Chem 58, 7940-8. https://doi.org/10.1021/jf100339p
  38. Wong CG, Bottiglieri T, and Snead OC (2003) GABA, gammahydroxybutyric acid, and neurological disease. Ann Neurol 54, 3-12.
  39. Zazzeroni R, Homan A, and Thain E (2009) Determination of a-aminobutyric acid in food matrices by isotope dilution hydrophilic interaction chromatography coupled to mass spectrometry. J Chromatogr Sci 47, 564-8. https://doi.org/10.1093/chromsci/47.7.564
  40. Zhang LL, Guan ZZ, and Zhang JK (2003) Experimental study of the effects of Asarinin on Immunosuppression activity in vitro. Chin J Cardiol 31, 444-7.
  41. Zhao M, Wang Y, and Kang L (2001) Study on activity of inner inhibitory substances of Acanthopanax senticosus (Rupr. et Maxim) harms fruits and seeds. China J Chin Mater Med 26, 534-8.
  42. Zhishen J, Mengcheng T, and Jianming W (1999) The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem 64, 555-9. https://doi.org/10.1016/S0308-8146(98)00102-2
  43. Zhou CC (1985) Anti-inflammatory action of ethanol extracts from Acanthopanax sessiliflorus. Zhong Yao Tong Bao 10, 37-41.
  44. Zhou DW and Li CM (1997) The pharmacological activity of the root bark of Morus alba L. World Notes Plant Med 12, 155-7.
  45. Zhu QG, Sujari ANA, and Ghani SA (2013) Nafion-MWCNT Composite Modified Graphite Paste for the Analysis of Quercetin in Fruits of Acanthopanax sessiliflorus. Sens Actuator B-Chem 177, 103-10. https://doi.org/10.1016/j.snb.2012.10.112

Cited by

  1. GABA, a non-protein amino acid ubiquitous in food matrices vol.4, pp.1, 2018, https://doi.org/10.1080/23311932.2018.1534323