Journal of Mushroom (한국버섯학회지)

The Korean Society of Mushroom Science (한국버섯학회)
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Nutritional contents and physiological activity of Pleurotus eryngii by extraction solvents

추출용매에 따른 큰느타리 버섯의 영양성분 및 생리활성

  • 박혜성 (농촌진흥청 국립원예특작과학원 인삼특작부 버섯과) ;
  • 김수연 (농촌진흥청 국립원예특작과학원 인삼특작부 버섯과) ;
  • 김희순 (농촌진흥청 국립원예특작과학원 인삼특작부 버섯과) ;
  • 한재구 (농촌진흥청 국립원예특작과학원 인삼특작부 버섯과) ;
  • 이강효 (농촌진흥청 국립원예특작과학원 인삼특작부 버섯과) ;
  • 조재한 (농촌진흥청 국립원예특작과학원 인삼특작부 버섯과)
  • Received : 2015.11.27
  • Accepted : 2015.12.23
  • Published : 2015.12.31
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Abstract

Physiological activities of 70% methanol, fermented ethanol and hot-water extracts of Pleurotus eryngii were investigated. Free radical scavenging activities of P. eryngii extracts were determined according to the elimination of DPPH radicals. Their nitrite scavenging activity and total polyphenol content were also determined. Amino acid analysis showed that phenylalanine (Phe) and glutamic acid (Glu) are most abundant essential and non-essential amino acids in the analyzed extracts. The hot-water extract of ASI 2394 represented the highest antioxidant activity with the DPPH radical scavenging rate value of $40.97{\pm}1.65%$. ASI 2820 displayed the superior capacity to eliminate nitrate regardless of extraction solvents. The hot-water extract of ASI 2887 had the highest content of polyphenol. Our results showed that P. eryngii is well qualified as a functional food.

큰느타리버섯의 아미노산을 함량을 분석하고, 70% 메탄올, 95% 발효주정, 물 추출물의 항산화 활성을 측정하였다. 아미노산 17종류 분석결과 필수아미노산에서는 체내 단백질을 구성하는 Phe의 함량이 가장 높았고, 비필수아미노산에서는 감칠맛을 내는 Glu가 가장 높은 함량을 보였다. DPPH 라디칼 소거능에서는 ASI 2887이 70% 메탄올, 95% 발효주정, 열수 추출용매별로 $34.37{\pm}1.19$, $34.66{\pm}5.46$, $40.97{\pm}1.65mg/g$으로 소거능을 보였고, 아질산염 소거능 역시 열수추출물이 다른 추출물보다 소거능이 높았으며 ASI 2820이 70% 메탄올, 95% 발효주정, 열수 추출물에서 각각 $21.45{\pm}2.73$, $33.32{\pm}1.06$, $33.31{\pm}2.65mg/g$으로 추출용매에 상관없이 높은 아질산염 소거능을 보였다. 폴리페놀 함량도 열수 추출물에서 가장 높은 함량을 보였고 가장 높은 함량을 보인 것은 ASI 2887로 DPPH 라디칼 소거능과 유사한 관계를 보이는 것을 확인하였다. 큰느타리버섯은 아미노산과 생리활성 물질의 다량 함유하고 있어 기능성 식품 및 건강식품으로서의 활용이 기대된다.

Keywords

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