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Inhibitory Effect of Rosa multiflora hip Extract on UVB-induced Skin Photoaging in Hs68 Fibroblasts

자외선으로 유도된 Hs68 섬유아세포의 노화 반응에 대한 영실추출물의 억제 효능

  • Received : 2015.10.21
  • Accepted : 2015.11.11
  • Published : 2015.12.30

Abstract

Acute and chronic ultraviolet (UV) irradiation triggers severe skin photoaging processes, which directly disrupt the normal three-dimensional integrity of skin. UV light stimulates the expression of matrix metalloproteinases (MMPs) which degrade constituents of extracellular matrix (ECM) proteins. These MMPs reduce collagen synthesis and decrease skin elasticity and integrity, resulting in wrinkle formation. In this study, we identified Rosa multiflora hip extract (RME) as an effective anti-photoaging ingredient. First, cell proliferation activity of RME was verified using Hs68 human dermal fibroblast cell line. RME downregulated MMPs expression through the inhibition of activator protein (AP)-1. In addition, type I and IV collagen expressions were increased with RME treatment and UVB-induced inflammatory responses were also reduced after RME treatment. In conclusion, R. multiflora hip extract may effectively improve UVB-induced skin aging and wrinkle formation which may provide as an anti-aging, anti-wrinkle, and anti-inflammation ingredient in cosmetic industry.

극심하고 지속적인 자외선에의 노출은 정상적인 피부구조를 파괴하는 다양한 피부 광노화 과정을 야기한다. 자외선은 인체 피부에서 세포외기질의 구성성분을 분해시키는 기질 분해효소인 matrix metalloproteinases(MMPs)의 발현을 활성화시키고, 콜라겐 합성은 감소시킴으로써 피부의 탄력과 구조적 치밀도를 약화시켜 궁극적으로 피부주름을 생성한다. 본 연구에서는 이러한 피부 광노화 현상을 완화시키는 소재로서 영실의 효능을 검증하고자 하였다. 먼저 인간 섬유아세포주인 Hs68을 이용하여 영실의 세포증식 촉진효능을 확인하였다. 여기에 더해 영실이 activator protein (AP)-1 전사인자의 억제를 통해 MMP의 발현을 감소시킴을 mRNA 및 단백질 수준에서 검증하였다. 또한, 진피층을 구성하는 타입 I형 콜라겐과 표피-진피 경계부를 단단히 고정시키는 역할을 하는 타입 IV형 콜라겐 역시 영실에 의해 발현이 증가하며, 자외선에 의한 염증반응의 억제에도 영실이 효과적으로 작용하는 것을 확인할 수 있었다. 결론적으로 본 연구를 통해 영실이 자외선에 의한 피부노화와 주름생성을 효과적으로 개선할 수 있는 가능성을 가짐으로써 항노화, 항염증 및 항주름 소재로서 화장품에 응용될 수 있을 것으로 기대된다.

Acknowledgement

Supported by : 보건복지부

References

  1. E. C. Naylor, R. E. B. Watson, and M. J. Sherratt, Molecular aspects of skin ageing, Maturitas, 69(3), 249 (2011). https://doi.org/10.1016/j.maturitas.2011.04.011
  2. M. A. Farage, K. W. Miller, P. Elsner, and H. I. Maibach, Intrinsic and extrinsic factors in skin ageing: a review, Int. J. Cosmet. Sci., 30(2), 87 (2008). https://doi.org/10.1111/j.1468-2494.2007.00415.x
  3. R. Pandel, B. Poljsak, A. Godic, and R. Dahmane, Skin photoaging and the role of antioxidants in its prevention, ISRN Dermatol., 2013, 930164 (2013).
  4. R. Pallela, Y. Na-Young, and S. K. Kim, Anti-photoaging and photoprotective compounds derived from marine organisms, Mar. Drugs, 8(4), 1189 (2010). https://doi.org/10.3390/md8041189
  5. T. Quan, Z. Qin, W. Xia, Y. Shao, J. J. Voorhees, and G. J. Fisher, Matrix-degrading metalloproteinases in photoaging, J. Investig. Dermatol. Symp. Proc., 14(1), 20 (2009). https://doi.org/10.1038/jidsymp.2009.8
  6. Y. Xu and G. J. Fisher, Ultraviolet (UV) light irradiation induced signal transduction in skin photoaging, J. Dermatol. Sci. Suppl., 1(2), S1 (2005). https://doi.org/10.1016/j.descs.2005.06.002
  7. A. Kammeyer and R. M. Luiten, Oxidation events and skin aging, Ageing Res. Rev., 21, 16 (2015). https://doi.org/10.1016/j.arr.2015.01.001
  8. A. M. A. Velez and M. S. Howard, Collagen IV in normal and in disease process, N. Am. J. Med. Sci., 4(1), 1 (2012). https://doi.org/10.4103/1947-2714.92892
  9. B. C. Yu, D. S. Lee, S. M. Bae, W. K. Jung, J. H. Chun, S. H. Urm, D. Y. Lee, S. J. Heo, S. G. Park, S. K. Seo, J. W. Yang, J. S. Choi, W. S. Park, and I. W. Choi, The effect of cilostazol on the expression of matrix metalloproteinase-1 and type I procollagen in ultraviolet-irradiated human dermal fibroblasts, Life Sci., 92(4-5), 282 (2013). https://doi.org/10.1016/j.lfs.2012.12.011
  10. C. D. Buckley, Why does chronic inflammation persist: an unexpected role for fibroblasts, Immunol. Lett., 138(1), 12 (2011). https://doi.org/10.1016/j.imlet.2011.02.010
  11. A. M. Manicone and J. K. McGuire, Matrix metalloproteinases as modulators of inflammation, Semin. Cell Dev. Biol., 19(1), 34 (2008). https://doi.org/10.1016/j.semcdb.2007.07.003
  12. C. Lopez-Camarillo, E. A. Ocampo, M. L. Casamichana, C. Perez-Plasencia, E. Alvarez-Sanchez, and L. A. Marchat, Protein kinases and transcription factors activation in response to UV-radiation of skin: implications for carcinogenesis, Int. J. Mol. Sci., 13(1), 142 (2012). https://doi.org/10.3390/ijms13010142
  13. J. Wu, X. Liu, C. Chan, D. K. W. Mok, S. Chan, Z. Yu, and S. Chen, Petroleum ether extractive of the hips of Rosa multiflora ameliorates collagen-induced arthritis in rats, J. Ethnopharmacol., 157, 45 (2014). https://doi.org/10.1016/j.jep.2014.09.026
  14. D. Guo, L. Xu, X. Cao, Y. Guo, Y. Ye, C. O. Chan, D. K. W. Mok, Z. Yu, and S. Chen, Anti-inflammatory activities and mechanisms of action of the petroleum ether fraction of Rosa multiflora Thunb. hips, J. Ethnopharmacol., 138(3), 717 (2011). https://doi.org/10.1016/j.jep.2011.10.010
  15. G. Q. Zhang, X. D. Huang, H. Wang, A. K. N. Leung, C. L. Chan, D. W. F. Fong, and Z. L. Yu, Anti-inflammatory and analgesic effects of the ethanol extract of Rosa multiflora Thunb. hips, J. Ethnopharmacol., 118(2), 290 (2008). https://doi.org/10.1016/j.jep.2008.04.014
  16. P. Sertznig and J. Reichrath, Peroxisome proliferator- activated receptors (PPARs) in dermatology: challenge and promise, Dermatoendocrinol., 3(3), 130 (2011). https://doi.org/10.4161/derm.15025
  17. M. Schmuth, V. Moosbrugger-Martinz, S. Blunder, and S. Dubrac, Role of PPAR, LXR, and PXR in epidermal homeostasis and inflammation, Biochim. Biophys. Acta-Mol. Cell Biol. Lipids, 1841(3), 463 (2014). https://doi.org/10.1016/j.bbalip.2013.11.012
  18. M. H. Park, J. Y. Park, H. J. Lee, D. H. Kim, K. W. Chung, D. Park, H. O. Jeong, H. R. Kim, C. H. Park, S. R. Kim, P. Chun, Y. Byun, H. R. Moon, and H. Y. Chung, The Novel PPAR $\alpha/\gamma$ Dual Agonist MHY 966 Modulates UVB-induced skin inflammation by inhibiting NF-${\kappa}B$ activity, PLoS One, 8(10), 4 (2013).
  19. Y. Jeon, Y. Jung, M. C. Kim, H. C. Kwon, K. S. Kang, Y. K. Kim, and S. N. Kim, Sargahydroquinoic acid inhibits TNF$\alpha$-induced AP-1 and NF-${\kappa}B$ signaling in HaCaT cells through PPAR$\alpha$ activation, Biochem. Biophys. Res. Commun., 450(4), 1553 (2014). https://doi.org/10.1016/j.bbrc.2014.07.026
  20. C. Franceschi, M. Capri, D. Monti, S. Giunta, F. Olivieri, F. Sevini, M. P. Panourgia, L. Invidia, L. Celani, M. Scurti, E. Cevenini, G. C. Castellani, and S. Salvioli, Inflammaging and anti-inflammaging: a systemic perspective on aging and longevity emerged from studies in humans, Mech. Ageing Dev., 128(1), 92 (2007). https://doi.org/10.1016/j.mad.2006.11.016