Asian-Australasian Journal of Animal Sciences

  • 제28권12호
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  • Pages.1721-1728
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  • 2015
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  • 1011-2367(pISSN)
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  • 1976-5517(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
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Characterization of Bovine NANOG5'-flanking Region during Differentiation of Mouse Embryonic Stem Cells

  • Jang, Hye-Jeong (Laboratory of Developmental Genetics, Department of Biomedical Sciences, Inha University School of Medicine) ;
  • Park, Hwan Hee (Laboratory of Developmental Genetics, Department of Biomedical Sciences, Inha University School of Medicine) ;
  • Tran, Thi Thuy Linh (Laboratory of Developmental Genetics, Department of Biomedical Sciences, Inha University School of Medicine) ;
  • Lee, Hak-Kyo (Department of Animal Biotechnology, Chonbuk National University) ;
  • Song, Ki-Duk (Department of Animal Biotechnology, Chonbuk National University) ;
  • Lee, Woon Kyu (Laboratory of Developmental Genetics, Department of Biomedical Sciences, Inha University School of Medicine)
  • 투고 : 2015.06.08
  • 심사 : 2015.08.12
  • 발행 : 2015.12.01
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초록

Embryonic stem cells (ESCs) have been used as a powerful tool for research including gene manipulated animal models and the study of developmental gene regulation. Among the critical regulatory factors that maintain the pluripotency and self-renewal of undifferentiated ESCs, NANOG plays a very important role. Nevertheless, because pluripotency maintaining factors and specific markers for livestock ESCs have not yet been probed, few studies of the NANOG gene from domestic animals including bovine have been reported. Therefore, we chose mouse ESCs in order to understand and compare NANOG expression between bovine, human, and mouse during ESCs differentiation. We cloned a 600 bp (-420/+181) bovine NANOG 5'-flanking region, and tagged it with humanized recombinant green fluorescent protein (hrGFP) as a tracing reporter. Very high GFP expression for bovine NANOG promoter was observed in the mouse ESC line. GFP expression was monitored upon ESC differentiation and was gradually reduced along with differentiation toward neurons and adipocyte cells. Activity of bovine NANOG (-420/+181) promoter was compared with already known mouse and human NANOG promoters in mouse ESC and they were likely to show a similar pattern of regulation. In conclusion, bovine NANOG 5-flanking region functions in mouse ES cells and has characteristics similar to those of mouse and human. These results suggest that bovine gene function studied in mouse ES cells should be evaluated and extrapolated for application to characterization of bovine ES cells.

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참고문헌

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