DOI QR코드

DOI QR Code

Effect of Physical Pre-treatment of Mature Seed in Callus Formation and Plant Regeneration of Zoysiagrass

잔디종자의 물리적 전처리가 캘러스 형성과 식물체 재분화에 미치는 효과

  • Ahn, Na-Young (Division of Applied Life Science (BK21 Plus), IALS, Gyeongsang National University) ;
  • Kim, Yong-Goo (Division of Applied Life Science (BK21 Plus), IALS, Gyeongsang National University) ;
  • Rahman, Md. Atikur (Division of Applied Life Science (BK21 Plus), IALS, Gyeongsang National University) ;
  • Bae, Eun Ji (Southern Forest Resources Research Center, National Institute of Forest Science) ;
  • Choi, Su Min (Southern Forest Resources Research Center, National Institute of Forest Science) ;
  • Lee, Kwang Soo (Southern Forest Resources Research Center, National Institute of Forest Science) ;
  • Lee, Byung-Hyun (Division of Applied Life Science (BK21 Plus), IALS, Gyeongsang National University)
  • 안나영 (경상대학교 응용생명과학부 (BK21 Plus), 농업생명과학연구원) ;
  • 김용구 (경상대학교 응용생명과학부 (BK21 Plus), 농업생명과학연구원) ;
  • 라하만 아티쿨 (경상대학교 응용생명과학부 (BK21 Plus), 농업생명과학연구원) ;
  • 배은지 (국립산림과학원 남부산림자원연구소) ;
  • 최수민 (국립산림과학원 남부산림자원연구소) ;
  • 이광수 (국립산림과학원 남부산림자원연구소) ;
  • 이병현 (경상대학교 응용생명과학부 (BK21 Plus), 농업생명과학연구원)
  • Received : 2015.11.17
  • Accepted : 2015.12.01
  • Published : 2015.12.31

Abstract

In order to improve the transformation efficiency of zoysiagrass by increasing the frequency of callus formation from mature seeds and plant regeneration, the effect of pre-treatment with sea sand was examined. Mature zoysiagrass seeds were given 10 min of swelling time before sea sand treatment using a sea sand and seed ratio of 1 : 1 and a vortex shaking speed of 6 (1,000 rpm) for 10 min. The seeds showed increased callus formation that was more than 2 times the rate in the control. In addition, plant regeneration efficiency was also increased when embryogenic callus induced from sea sand-treated mature seeds was cultured in regeneration medium. These results will be very helpful for improving the genetic transformation frequency of zoysiagrass, a recalcitrant monocot grass.

잔디에 있어서 낮은 형질전환 효율의 직접적인 원인 중에 하나인 낮은 캘러스 유도율과 식물체 재분화율을 개선시키기 위하여 잔디 완숙종자의 전처리의 효과를 비교해 보았다. 살균한 잔디종자에 swelling 처리를 10분간 실시한 후, sea sand를 이용 하여 sea sand와 종자의 비율을 1:1로 한 다음, shaking 처리는 vortex speed 6 (1,000 rpm)에서 처리시간은 10분간 전처리를 실시하였을 때 무처리구에 비해 2배 이상의 배양 효율을 나타내었다. 또한 sea sand로 전처리한 잔디종자로부터 형성된 캘러스를 식물체 재분화 유도배지에 치상했을 그 효율이 훨씬 더 증가하는 것을 확인할 수 있었다. 이러한 결과는 연중 아무 때나 이용할 수 있는 장점을 지닌 잔디 완숙종자로부터 유도시킨 캘러스 세포를 이용하여 Agrobacterium으로 형질전환하는데 있어서 그 효율을 향상 시키는데 큰 기여를 할 것으로 추측된다.

Keywords

References

  1. Carmo-Silva, A.E., Keys, A.J., Beale, M.H., Ward, J.L., Baker, J.M., Hawkins, N.D., Arrabaça, M.C. and Parry, M.A.J. 2009. Drought stress increases the production of 5-hydroxynorvaline in two C4 grasses. Phytochemistry 70:664-671. https://doi.org/10.1016/j.phytochem.2009.03.001
  2. Choi, C.H. 2012. Effect of temperature and various pre-treatments on germiantion of Hippophae rhamnoides seeds. Korean Journal of Plant Research 25:132-141. https://doi.org/10.7732/kjpr.2012.25.1.132
  3. Dhital, D., Yashiro, Y., Ohtsuka, T., Noda, H., Shizu, Y. and Koizumi, H. 2010. Carbon dynamics and budget in a Zoysia japonica grassland, central Japan. Journal of Plant Research 123:519-530. https://doi.org/10.1007/s10265-009-0289-6
  4. Han, J.J., Lee, G.S., Park, Y.B., Yang, K.M. and Bae, E.J. 2014. Comparison of germination characteristics and various pretreatment methods for enhancing germination on zoysiagrass. Weed & Turfgrass 3(3): 232-239. https://doi.org/10.5660/WTS.2014.3.3.232
  5. Ito, T.Y. and Takatsuki, S. 2005. Relationship between a high density of sika deer and productivity of the short-grass (Zoysia japonica) community: a case study on Kinkazan Island, northern Japan. Ecology Research 20:573-579. https://doi.org/10.1007/s11284-005-0073-6
  6. Kang, K.S., Ahn, C.K., Lee, J.W., Choi, Y.W., Son, B.G., et al. 2005. Effects of seed coat scarification on improved germination in Korean lawngrass. Korean Journal of Horticulture Science and Technology 21:75 (Abstract). (In Korean)
  7. Murashige, T. and Skoog, F. 1962. A revise medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15:473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  8. Osborne, C. P. and Freckleton, R. P. 2009. Ecological selection pressures for C4 photosynthesis in the grasses. Proceedings of the Royal Society B: Biological Sciences 276:1753-1760. https://doi.org/10.1098/rspb.2008.1762
  9. Park, M. Y. 2013. Improvement of transformation protocol for Zoysia japonica Steud. and production of transgenic zoysiagrass introducing abiotic stress-related genes. Ph.D. thesis, Jeju, Korea.
  10. Sugihara, S., Kobayashi, M., Ebina, M., Tsurumi, Y., Ooya, I. and Nashiki, M. 1999. Review of researches concerning Zoysia in Japan. Grassland Science 45:105-112.
  11. Sun, H.J., Song, I.J., Bae, T.W. and Lee, H.Y.. 2010. Recent developments in biotechnological improvement of Zoysia japonica Steud. Journal of Plant Biotechnology 37:400-407. https://doi.org/10.5010/JPB.2010.37.4.400
  12. Toyama, K., Bae, C.H., Kang, J.G., Lim, Y.P., Adachi, T., Riu, K.Z., Song, P.S. and Lee, H.Y. 2003. Production of herbicide-tolerant zoysiagrass by Agrobacterium-mediated transformation. Molecules and Cells 16:19-27.
  13. Sim, K.Y., Kim, H.J., Ham, S.K., Choi, J.S. and Sim, S.R. 1998. Construction and management of turfgrass ground. In Research Report of Korea Institute of Sport Science. Seoul. pp.95-97.