Journal of The Korean Society of Grassland and Forage Science (한국초지조사료학회지)

The Korean Society of Grassland and Forage Science (한국초지조사료학회)
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Effect of Addition of Lactic Acid Bacteria on Fermentation Quality of Rye Silage

Lactobacillus plantarum 첨가가 호밀 사일리지의 발효 품질에 미치는 영향

  • Received : 2015.06.01
  • Accepted : 2015.10.02
  • Published : 2015.12.31
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Abstract

This study was conducted to investigate the effect of novel Lactobacillus plantarum KCC-10 and KCC-19 on the quality and fermentation characterization of rye silages. The study was conducted at the National Institute of Animal Science, Cheonan province in Korea and consisted of three treatments: control without lactic acid bacteria, treatment with L. plantarum KCC-10 and treatment with L. plantarum KCC-19. The amounts of acid detergent fiber and neutral detergent fiber as well as the in vitro dry matter digestibility in KCC-10 and KCC-19 were similar to the control. The pH of rye silage in L. plantarum KCC-10 and KCC-19 treatments decreased compared to the control (p<0.05). The amount of lactic acid in L. plantarum KCC-10 and KCC-19 treatments increased (p<0.05), but the amounts of acetic acid and butyric acid in KCC-10 and KCC-19 treatments decreased (p<0.05). In addition, the number of lactic acid bacteria in L. plantarum KCC-10 and KCC-19 treatments increased compared to the control (p<0.05). Therefore, we suggest that rye silage was improved by the addition of L. plantarum KCC-10 and KCC-19.

본 연구에서는 호밀을 이용하여 사일리지 제조시 Lactobacillus plantarum KCC-10 및 KCC-19를 접종하여 사일리지의 사료가치, 품질 및 미생물상의 변화를 조사하였다. 호밀 사일리지에서 젖산균 접종구와 무접종구에서 사료가치는 크게 변화되지 않았다. 그리고 유산 함량은 젖산균 접종구가 현저하게 높은 경향을 보였으나 초산과 낙산은 젖산균 접종구가 약간 낮아지는 경향을 보였다. 젖산균 분포는 젖산균 접종구에서 현저하게 높았으며 효모와 곰팡이는 $10^4CFU/g$에서 관찰되지 않았다. 호밀 사일리지 제조시 KCC-10 및 KCC-19 접종구의 조단백질 및 TDN 함량은 무접종구 보다 약간 증가하였으나 섬유소 및 in vitro 건물소화율에서는 비슷한 수준을 보였다. 이상의 결과를 요약해 보면 호밀 사일리지 제조시 KCC-10 및 KCC-19 접종에 의해 사일리지의 품질이 향상되었다.

Keywords

References

  1. Amanullah, S.M., Kim, D.H., Lee, H.J., Joo, Y.H., Kim, S.B. and Kim, S.C. 2014. Effects of microbial additives on chemical composition and fermentation characteristics of barley silage. Asian-Australasian Journal of Animal Science. 27:511-517. https://doi.org/10.5713/ajas.2013.13617
  2. Anderson, R., Gracey, H.I., Kennedy, S.J., Unsworth E.F. and Steen, R.W.J. 1989. Evaluation studies in the development of a commercial bacterial inoculant as an additive for grass silage. Grass and Forage Science. 44:361-369. https://doi.org/10.1111/j.1365-2494.1989.tb01934.x
  3. AOAC. 1990. Official method of analysis. 15thed. Washington, DC.
  4. Choi, K.C., Jo, N.C., Jung, M.W., Lee, K.D., Kim, J.G., Lim, Y.C., Kim, W.H., Oh, Y.K., Choi, J.H., Kim, C.M., Jung, D.K., Choi, J.M. and Kim, H.G. 2011a. Effect of harvest stage of corn on nutritive values and quality of roll baled corn silage manufactured with corn grown in paddy land. Journal of the Korean Society of Grassland and Forage Science. 31:65-74. https://doi.org/10.5333/KGFS.2011.31.1.65
  5. Choi, K.C., Jung, M.W., Kim, W.H., Kim, C.M., Yoon, S.H., Choi E.M., Kim, J.G., Lee, S.M., Choi, J.M., Kim, H.G. and Lim, Y.C. 2011b. Effect of harvest Stage of Sorghum $\times$ Sorghum Hybrid (SSH) on the quality of round baled SSH silage. Journal of the Korean Society of Grassland and Forage Science. 31:143-150. https://doi.org/10.5333/KGFS.2011.31.2.143
  6. Choi, K.C., Valan Arasu, M., Ilavenil, S., Park, H.S., Jung, M.W. Kim, J.H., Jung, J.S., Hwangbo, S., Kim, W.H. and Lim, Y.C. 2014a. Effect of addition of lactic acid bacteria and chlorella on nutritive values and quality of Italian ryegrass-hairy vetch silage. Journal of the Korean Society of Grassland and Forage Science. 34:39-44. https://doi.org/10.5333/KGFS.2014.34.1.39
  7. Choi, K.C., Valan Arasu, M., Ilavenil, S., Park, H.S., Jung, M.W. and Lee, S.H. 2014b. Effect of lactic acid bacteria and chlorella on nutritive values and quality of Italian Ryegrass-alfalfa silage. Journal of the Korean Society of Grassland and Forage Science. 34:33-38. https://doi.org/10.5333/KGFS.2014.34.1.33
  8. Dewar, W.A., McDonald, P. and Whittenbury, R. 1963. The hydrolysis of grass hemicelluloses during ensilage. Journal of the Science of Food and Agriculture. 14:411-417. https://doi.org/10.1002/jsfa.2740140610
  9. Dogi, C.A.1., Pellegrino, M., Poloni, V, Polon, I.L., Pereyra, C.M., Sanabria, A., Pianzzola, M.J., Dalcero, A. and Cavaglieri, L. 2015. Efficacy of corn silage inoculants on the fermentation quality under farm conditions and their influence on aspergillus parasitucus, A. flavus and A. fumigatus determined by q-PCR. Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment 32:229-235. https://doi.org/10.1080/19440049.2014.986223
  10. Goering, H.K. and Van Soest, P.J. 1970. Forage fiber analysis. Agic. Handbook 379, U. S. Gov. Print. Office, Washington, DC.
  11. Hu, X., Hao, W., Wang, H., Ning, T., Zheng, M. and Xu, C. 2015. Fermentation characteristics and lactic acid bacteria succession of total mixed ration silages formulated with peach pomace. Asian-Australasian Journal of Animal Science. 28:502-510. https://doi.org/10.5713/ajas.14.0508
  12. Jeon, W.T., Seong, ,K.Y., Lee, J.K., Kim, M.T. and Cho, H.S. 2009. Effects of seeding rate on hairy vetch (Vicia villosa)-rye (Secale cereale) mixtures for green manure production in upland soil. Korean Journal of Crop Science. 54:327-331.
  13. Ju, J.I., Lee, S.S., Yoo, J.H., Lee, J.J., Park, K.H. and Lee, H.B. 2008. Seed blending effect on growth, yield and feed value among four winter cereals for whole crop silage. Journal of the Korean Society of Grassland and Forage Science. 28:203-214. https://doi.org/10.5333/KGFS.2008.28.3.203
  14. Ju, J.I., Choi H.G, Kang, Y.S., Lee, J.J., Park, K.H. and Lee, H.B. 2009. Changes of growth and forage yield at different cutting dates among five winter cereals for whole crop silage in middle region. Journal of the Korean Society of Grassland and Forage Science. 29:111-120. https://doi.org/10.5333/KGFS.2009.29.2.111
  15. Kennedy, S.J., Gracey, H.I., Unsworth, E.F., Steen, R.W.J. and ANderson, R. 1989. Evaluation studies in the development of a commercial bacterial inoculant as an additive for grass silage. 2. Responses in finishing cattle. Grass and Forage Science. 44:371-380. https://doi.org/10.1111/j.1365-2494.1989.tb01935.x
  16. Kang, W.S., Kim, G.G., Chung, E.S., Ham, J.S., Kim, J.D. and Kim, K.N. 1999. Studies on Improvement of Quality of Round Bale Silage Using Fresh Rice Straw. Journal of the Korean Society of Grassland and Forage Science. 19:41-48.
  17. Kwon, Y.U., Baek, S.B., Heo, H.Y., Park, H.H., Kim, J.G., Lee, J.E., Lee, C.K. and Shin, J.C. 2008. Changes in forage quality of plant parts with harvesting time in five winter cereal Crops. Korean Journal of Crop Science. 53:144-149.
  18. Keady, T.W.J. and Steen, R.W.J. 1994. Effects of treating low dry-matter grass with a bacterial inoculant on the intake and performance of beef cattle and studies on its mode of action. Grass and Forage Science. 49:438-446. https://doi.org/10.1111/j.1365-2494.1994.tb02021.x
  19. Kim, J.G., Chung, E.S., Kim, M.J., Seo, S., Lee, J.K., Kim, J.D. and Seo, J.H. 2005. Effect of seeding dates and rates on the productivity and nutritive value of hairy vetch(Vicia cillosa Roth). Journal of the Korean Society of Grassland and Forage Science. 25:17-22. https://doi.org/10.5333/KGFS.2005.25.1.017
  20. Kim, K.M., Lee, B.J. and Cho, Y.S. 2012. Differences of soil carbon by green manure crops in rotated cropping system. Korean Journal of Soil Science and Fertilizer. 45:1027-1031. https://doi.org/10.7745/KJSSF.2012.45.6.1027
  21. Kim, J.G., Seo, S. Jung, E.S., Kang, W.S., Ham, J.S. and Kim, D.A. 2000. Effect of maturity at harvest on the changes in nutritive value of round baled rye silage. Journal of the Korean Society of Grassland and Forage Science. 20:309-316.
  22. Kim, J.G., Kim, D.A., Jung, E.S., Kang, W.S., Ham, J.S. and Seo. S. 1999. Effect of maturity at harvest and inoculants on the quality of round baled rye silage. Journal of the Korean Society of Grassland and Forage Science. 19:347-354.
  23. Kim, M.J., Choi, K.J., Kim, J.G., Seo, S., Yoon, S.H., Lim, Y.C., Im, S.K., Kwon, E.G., Chang, S.S., Kim, H.C. and Kim, T.I. 2009. Effect of varieties and seeding date on over winter and dry matter yield of Italian ryegrass in paddy field. Journal of the Korean Society of Grassland and Forage Science. 29:321-328. https://doi.org/10.5333/KGFS.2009.29.4.321
  24. Lee, K.N. and Kim, D.A. 1997. Effects of wilting and additives on the fermentation chrateristics, quality and aerobic Sstability of rye silage. Journal of the Korean Society of Grassland and Forage Science. 17:187-198.
  25. Liu, Q., Chen, M., Zhang, J., Sh,i S. and Cai, Y. 2012. Characteristics of isolated lactic acid bacteria and their effectiveness to improve stylo (Stylosanthes guianensis Sw.) silage quality at various temperatures. Animal Science Journal. 83:128-135. https://doi.org/10.1111/j.1740-0929.2011.00937.x
  26. Ilavenil, S., Valan Arasu, M., Vijayakumar, M., Jung, M.W., Park, H.S., Lim, Y.C. and Choi, K.C. 2014. Lactobacillus plantarum improves the nutritional quality of Italian Ryegrass with alfalfa mediated silage. Journal of the Korean Society of Grassland and Forage Science. 34:174-178. https://doi.org/10.5333/KGFS.2014.34.3.174
  27. Mayne, C.S. 1990. An evaluation of an inoculant of Lactobacillus plantarum as an additive for grass silage for dairy cattle. Animal Production. 51:1-13. https://doi.org/10.1017/S0003356100005109
  28. Moore, J.E. 1970. Procedure for the two-stage in vitro digestion of forage. Univ. of Florida, Depart. of Anim. Sci.
  29. Muck, R.R. and Bolsen, K.K. 1991. Silage preservation and silage additives. p. 105-125. In Bolsen, K.K., Baylor, J.E. and McCullough, M.E. (eds) Hay and Silage Management in North America. Nat. Feed Ingreed. Assoc., West Des Moines, Iowa.
  30. Nelson, C.E. 1993. Strategies of mold control in dairy feeds. Journal of Dairy Science. 76:898-902. https://doi.org/10.3168/jds.S0022-0302(93)77416-0
  31. Oh, M.G., Jo, I.H. and Hwanfbo, S. 2014. Effect of mixed-sowing of legume and applying of cattle manure on the productivity, feed values and organic hanwoo carrying capacity of rye (Secale cereale L.). Korean Journal of Organic Agriculture. 22:457-468. https://doi.org/10.11625/KJOA.2014.22.3.457
  32. Pang, H., Zhang, M., Qin, G., Tan, Z., Li, Z., Wang, Y. and Cai, Y. 2011. Identification of lactic acid bacteria isolated from corn stovers. Animal Science Journal. 82:642-653. https://doi.org/10.1111/j.1740-0929.2011.00894.x
  33. Ramos, A.L., Torello, C.O. and Queiroz, M.L. 2010. Chlorella vulgaris modulates immunomyelopoietic activity and enhances the resistance of tumor-bearing mice. Nutrition Cancer. 62:1170-1180. https://doi.org/10.1080/01635581.2010.513801
  34. Smith, D.F. and Lynch, P.L. 1973. Aspergillus fumigatus in sample of moldy silage. Journal of Dairy Science. 56:828-829. https://doi.org/10.3168/jds.S0022-0302(73)85259-2
  35. Sebunya, T.K. and Yourtee, D.M. 1990. Aflatoxigenic aspergilli in foods and feeds in Uganda. Journal of Food Quality. 13:97-101. https://doi.org/10.1111/j.1745-4557.1990.tb00010.x
  36. Song, B.H., Lee, K.A., Jeon, W.T., Kim, M.T., Cho, H.S., Oh, I..S., Kim, C.G. and Kang, U.G. 2010. Effects of green manure crops of legume and gramineae on growth responses and yields in rice cultivation with respect to environment friendly agriculture. Journal of Crop Science. 55:144-150.
  37. Song, T.H., Han, O.K., Yun, S.K., Park, T.I., Seo, J.H., Kim, K.H. and Park, K.H. 2009. Changes in quantity and quality of winter cereal crops for forage at different growing stages. Journal of the Korean Society of Grassland and Forage Science. 29:129-136. https://doi.org/10.5333/KGFS.2009.29.2.129
  38. Sun, Q., Gao, F., Yu, Z., Tao, Y., Zhao, S. and Cai, Y. 2012. Fermentation quality and chemical composition of shrub silage treated with lactic acid bacteria inoculants and cellulase additives. Animal Science Journal. 83:305-309. https://doi.org/10.1111/j.1740-0929.2011.00962.x
  39. Sung, H.G., Lee, J.K. and Seo, S. 2011. Studies on fungal contamination and mycotoxins of rice straw round bale silage. Journal of the Korean Society of Grassland and Forage Science. 31:451-462. https://doi.org/10.5333/KGFS.2011.31.4.451
  40. Tohno, M., Kobayashi, H., Nomura, M., Uegaki, R. and Cai, Y. 2012a. Identification and characterization of lactic acid bacteria isolated from mixed pasture of timothy and orchardgrass, and its badly preserved silage. Animal Science Journal. 3:318-330.
  41. Tohno, M., Kobayashi, H., Nomura, M., Kitahara, M., Ohkuma, M., Uegaki, R. and Cai, Y. 2012b. Genotypic and phenotypic characterization of lactic acid bacteria isolated from Italian ryegrass silage. Animal Science Journal. 83:111-120. https://doi.org/10.1111/j.1740-0929.2011.00923.x
  42. Valan Arasu, M., Ilavenil, S., Jane, M., Kim, D.H., Lee, K.D., Park, H.S. and Choi K.C. 2014a. Effect of addition of chlorella with Lactobacillus plantarum on quality, microbial contents and fermentation metabolites of barley and pea silages. Journal of Pure and Applied Microbiology. 8:4017-4023.
  43. Valan Arasu, M., Jung, M.W., Ilavenil, S., Jane, M., Kim, D.H,. Lee, K.D,. Park, H.S., Huh, T.Y., Choi, G.J., Lim, Y.C., Al-Dhabi N.A. and Choi, K.C. 2013. Isolation and characterization of antifungal compound from Lactobacillus plantarum KCC-10 from forage silage with potential beneficial properties. Journal of Applied Microbiolology. 115:1172-1185. https://doi.org/10.1111/jam.12319
  44. Valan Arasu, M., Jung, M.W., Kim, D.H., Ilavenil, S., Lee, K.D., Choi, G.J., Al-Dhabi N.A. and Choi, K.C. 2014b. Isolation and characterization of Lactobacillus plantarum KCC-19 from crimson silage. Journal of Pure and Applied Microbiology. 8:3575-3587.
  45. Wu, J.J., Du, R.P., Gao, M, Sui, Y.Q., Xiu, L. and Wang, X. 2014. Naturally occurring lactic Acid bacteria isolated from tomato pomace silage. Asian-Australasian Journal of Animal Science. 27:648-657. https://doi.org/10.5713/ajas.2013.13670
  46. Yoon, S.H., Kim, J.G., Jung, E.S. and Lim, Y.C. 2007. The study on double cropping system for organic forage production in middle part of Korea. Journal of the Korean Society of Grassland and Forage Science. 27:275-280. https://doi.org/10.5333/KGFS.2007.27.4.275

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