Journal of Korean Society of Water and Wastewater (상하수도학회지)

The Korean Society of Water and Wastewater (대한상하수도학회)
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Development of Techniques for Evaluating the Virus Removal Rate using Adenovirus

아데노바이러스를 이용한 바이러스 제거율 평가를 위한 기법 개발

  • Cho, Yoonjung (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University) ;
  • Lim, Jaewon (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University) ;
  • Baek, Dawoon (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University) ;
  • Lee, Sang-Hoon (Department of Environmental Engineering, Yonsei University) ;
  • Lee, In-Soo (Department of Clinical Laboratory Science, Hyejeon College) ;
  • Lee, Hyeyoung (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University) ;
  • Park, Donghee (Department of Environmental Engineering, Yonsei University) ;
  • Jung, Dongju (Department of Biomedical Laboratory Science, College of Life and Health Sciences, Hoseo University) ;
  • Kim, Tae Ue (Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University)
  • 조윤정 (연세대학교 보건과학대학 임상병리학과) ;
  • 임재원 (연세대학교 보건과학대학 임상병리학과) ;
  • 백다운 (연세대학교 보건과학대학 임상병리학과) ;
  • 이상훈 (연세대학교 보건과학대학 환경공학과) ;
  • 이인수 (혜전대학교 임상병리과) ;
  • 이혜영 (연세대학교 보건과학대학 임상병리학과) ;
  • 박동희 (연세대학교 보건과학대학 환경공학과) ;
  • 정동주 (호서대학교 생명보건과학대학 임상병리학과) ;
  • 김태우 (연세대학교 보건과학대학 임상병리학과)
  • Received : 2015.09.24
  • Accepted : 2015.10.30
  • Published : 2015.12.15
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Abstract

Waterborne infectious disease is induced by several pathogenic microbes such as bacteria, viruses and protozoans, and the cases caused by viral infection is currently increasing. Water treatment process could reduce the number of virus in the water, but there were many difficulties to completely remove the virus particles from water. Therefore, the membrane separation technology which was reported to effectively remove pollutants from raw water has attracted increasing attention and demand. Since its efficiency has been introduced, demands for evaluation method toward the membrane filtration process are increasing. However, progression of the method development is slow due to the difficulties in cultivation of several waterborne viruses from animal models or cell culture system. To overcome the difficulties, we used adenovirus, one of the commonly isolated pathogenic waterborne viruses which can grow in cell culture system in vitro. The adenovirus used in this study was identified as human adenovirus C strain. The adenovirus was spiked in the raw water and passed through the microfiltration membrane produced by Econity, a Korean membrane company, and then the viral removal rate was evaluated by real-time PCR. In the results, the amount of virus in the filtered water was decreased approximately by 5 log scale. Because coagulant treatment has been known to reduce filtering function of the membrane by inducing fouling, we also investigated whether there was any interference of coagulant. In the results, we confirmed that coagulant treatment did not show significant interference on microfiltration membrane. In this study, we found that waterborne virus can be effectively removed by membrane filtration system. In particular, here we also suggest that real-time PCR method can rapidly, sensitively and quantitatively evaluate the removal rate of virus. These results may provide a standard method to qualifying membrane filtration processes.

Keywords

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