원예과학기술지 (Horticultural Science & Technology)

  • 제33권6호
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  • Pages.947-954
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  • 2015
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  • 1226-8763(pISSN)
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  • 2465-8588(eISSN)
한국원예학회 (Korean Society of Horticultural Science)
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스프레이 국화 형질전환을 위한 국내 육성 품종 선발

The Selection of Domestically Bred Cultivars for Spray-type Chrysanthemum Transformation

  • 서은정 (농촌진흥청 국립농업과학원 농업생명자원부 분자육종과) ;
  • 한봉희 (농업기술실용화재단 종자사업팀) ;
  • 이연희 (농촌진흥청 국립농업과학원 농업생명자원부 분자육종과) ;
  • 이성곤 (농촌진흥청 국립농업과학원 농업생명자원부 분자육종과) ;
  • 홍준기 (농촌진흥청 국립농업과학원 농업생명자원부 분자육종과) ;
  • 김경환 (농촌진흥청 국립농업과학원 농업생명자원부 분자육종과)
  • Suh, Eun-Jung (Molecular breeding Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Han, Bong Hee (Department of Seed Service, The Foundation of Agri. Tech. Commercialization and Transfer) ;
  • Lee, Yeon-Hee (Molecular breeding Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Lee, Seong-Kon (Molecular breeding Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Hong, Joon Ki (Molecular breeding Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, Kyung Hwan (Molecular breeding Division, National Academy of Agricultural Science, Rural Development Administration)
  • 투고 : 2015.02.13
  • 심사 : 2015.08.14
  • 발행 : 2015.12.31
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초록

질전환에 적합한 국화 스프레이 타입 품종을 선발하기 위해서 국내 연구기관에서 육성된 39개 품종 및 일본품종인 스탠다드 타입 신마를 모아 재분화율과 아그로박테리움 감염도를 조사하였다. 잎과 마디 절편체로부터의 신초 재분화를 위하여 IAA $0.5mg{\cdot}L^{-1}$와 BAP $1.0mg{\cdot}L^{-1}$가 첨가된 MS 배지를 사용하였다. 잎 절편체로부터의 신초 재분화는 BRM 품종이 가장 높았고 VS, WW, YTM 품종순으로 분화되었다. 이전에 형질전환재료로 보고된 바 있는 신마는 PK, SPP 품종과 유사한 수준을 보였다. 마디 절편체로부터의 신초 재분화는 PA 품종에서 가장 높았으며 WW 품종이 다음으로 확인되었다. 40개의 국화 품종을 대상으로 아그로박테리움에 대한 감염도를 조사한 결과, 잎 절편체에서는 WPP, YNW, VS, PP, WW, FA, PA, YMN 품종이 가장 높았고 마디 절편체에서는 WPP, PA, PK, YNW 품종이 가장 높았다. 이상의 결과를 종합해 볼 때 국화의 잎 절편체를 이용한 형질전환은 VS와 WW 품종이 가장 적합한 반면에 마디형질전환에는 PA 품종이 가장 적합한 것으로 확인되었다.

To select suitable spray chrysanthemum cultivars for Agrobacterium-mediated transformation, thirty-nine (39) spray cultivars bred in the National Institutes of Korea and a standard cultivar Jinba from Japan were collected and tested for regeneration rate and Agrobacterium infection assays. MS medium with $0.5mg{\cdot}L^{-1}$ IAA and $1.0mg{\cdot}L^{-1}$ BAP was used for shoot regeneration from leaf disks and internodes. The shoot regeneration rate in leaf disks was the highest in cultivar BRM, followed by cultivars VS, WW and YTM. The cultivar JB (Jinba) used as a transformation material in previous reports ranked similarly to cultivars PK and SPP. In shoot regeneration from internodes, the shoot regeneration rate was the highest for cultivar PA, followed by cultivar WW. The infection rate of leaves and internodes of 40 chrysanthemum cultivars with agrobacterium was investigated. Cultivars WPP, YNW, VS, PP, WW, FA, PA and YMN showed the highest infection levels in leaves, whereas cultivars WPP, PA, PK and YNW had the highest infection levels in internodes. Considering all of these results, cultivars VS and WW were the most appropriate for gene transformation of chrysanthemum using leaves, while cultivar PA was for internodes.

키워드

참고문헌

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피인용 문헌

  1. 주요 스프레이 국화 품종의 형태적 특성과 변이계수, 유전율 및 유전자 전이율 vol.34, pp.2, 2015, https://doi.org/10.12972/kjhst.20160028