DOI QR코드

DOI QR Code

Condition Setting for Oral Mucosal Irritation Evaluation using Hamster Cheek Pouch

햄스터 볼주머니를 이용한 구강점막 자극평가 조건설정

  • Park, Kyo-Hyun (Department of Public Health, College of Natural Sciences, Keimyung University) ;
  • Kim, Kwang-Mahn (Research Institute of Dental Biomaterials and Bioengineering, College of Dentistry Yonsei University) ;
  • Kim, Bae-Hwan (Department of Public Health, College of Natural Sciences, Keimyung University)
  • 박교현 (계명대학교 자연과학대학 공중보건학과) ;
  • 김광만 (연세대학교 치과대학 치과생체재료공학교실 및 연구소) ;
  • 김배환 (계명대학교 자연과학대학 공중보건학과)
  • Received : 2015.08.17
  • Accepted : 2015.12.14
  • Published : 2015.12.28

Abstract

Objectives: The purpose of this study is to evaluate the optimum conditions for oral mucosal irritation testing using the buccal pouch of hamsters. Methods: Test materials were applied to the buccal pouch of seven-week old male Syrian hamsters (SLC, Japan) four times at one-hour intervals and macroscopic changes were examined at 24 hours after final treatment. After sacrifice, the buccal pouches were removed and prepared for histopathological evaluation. In order to set the exposure time, we performed exposure tests of 5, 12, 18 and 23 minutes using sodium lauryl sulfate (SLS) 1% and set the treatment volume from the test results at 2, 3, or 4 ml treatment using SLS 1%, Triton X-100 1% and ethanol. After setting the experimental conditions, seven groups of materials [sodium lauryl sulfate (SLS) (1%), Triton X-100 (1%), hydrogen peroxide (3%), ethanol (100%), chlorhexidine (0.2%, 2%), phosphate buffer saline (PBS)] were assessed. Results: Experimental conditions of material exposure time were fixed as 18 minutes from the exposure tests of 5, 12, 18 or 23 min using sodium lauryl sulfate (SLS) 1%. Treated volume was set as 4 ml per each pouch from the test results of 2, 3, or 4 ml treatments using SLS 1%, Triton X-100 1% and ethanol. The results in terms of irritation degree were in the order of sodium lauryl sulfate (SLS) (1%) > Triton X-100 (1%) ${\fallingdotseq}$ hydrogen peroxide (3%) > ethanol (100%) ${\fallingdotseq}$ chlorhexidine (0.2%, 2%) > phosphate buffer saline (PBS). Conclusion: From this study, suitable conditions for hamster mucosal irritation testing were suggested and this method was verified through materials commonly used on oral mucosal membranes.

Keywords

References

  1. Rantanen I, Jutila K, Nicander I, Tenovuo J, Sderling E. The effects of two sodium lauryl sulphatecontaining toothpastes with and without betaine on human oral mucosa in vivo. Swed Dent J. 2003; 27(1): 31-34.
  2. Ahlfors EE, Dahl JE, Lyberg T. The development of T cell-dominated inflammatory responses induced by sodium lauryl sulphate in mouse oral mucosa. Arch Oral Biol. 2012; 57(6): 796-804. https://doi.org/10.1016/j.archoralbio.2011.11.005
  3. Ahlfors E, Czerkinsky C. Contact sensitivity in the murine oral mucosa. I. An experimental model of delayed-type hypersensitivity reactions at mucosal surfaces. Clin Exp Immunol. 1991; 86(3): 449-456. https://doi.org/10.1111/j.1365-2249.1991.tb02952.x
  4. Bourrinet P, Conduzorgues JP, Dutertre H, Macabies J, Masson P, Maurin J, et al. Interlaboratory study for the assessment of potential irritative properties of hygiene products on the hamster cheek pouch. Lab Anim Sci. 1995; 45(1): 36-40.
  5. Veys RJ, Baert JH, De Boever JA. Histological changes in the hamster cheek pouch epithelium induced by topical application of sodium lauryl sulphate. Int J Exp Pathol. 1994; 75(3): 203-209.
  6. Utoguchi N, Watanabe Y, Takase Y, Suzuki T, Matsumoto M. Carrier-mediated absorption of salicylic acid from hamster cheek pouch mucosa. J Pharm Sci. 1999; 88(1): 142-146. https://doi.org/10.1021/js970412e
  7. ISO. ISO 10993-10:2010 Biological evaluation of medical $devices^{{\circ}{TM}}$Part 10: Tests for irritation and skin sensitization.
  8. Jeong SH, Ok SM, Huh JY, Ko MY, Ahn YW. A Study for Direct Application of Drug on Oral Mucosa. Korean J Oral Med. 2010; 35(4): 229-235.
  9. Piliero SJ, Carson S, LiCalzi M, Pentel L, Piliero JA, Kaufman EG, et al. Biocompatibility evaluation of casting alloys in hamsters. J Prosthet Dent. 1979; 41(2): 220-223 https://doi.org/10.1016/0022-3913(79)90311-1
  10. Kim BH, Kim JW, Chang IS, Sim YC, Lee YS. Evaluation of irritation potential of newly-developed toothpaste in the hamster oral mucous membrane. J Toxicol Res. 2001; 17(3): 167-171.
  11. Namiki N, Takagi N, Yuasa H, Kanaya Y. Studies on development of dosage forms for pediatric use (V) oral mucosal irritation study of gummi drugs in hamster cheek pouch. Biol Pharm Bull. 1998; 21(1): 87-89. https://doi.org/10.1248/bpb.21.87
  12. Baert JH, Veys RJ, Ampe K, De Boever JA. The effect of sodium lauryl sulphate and triclosan on hamster cheek pouch musosa. Int J Exp Pathol. 1996; 77(2): 73-78. https://doi.org/10.1046/j.1365-2613.1996.00965.x