Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
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Topical application of herbal formula for the treatment of ligature-induced periodontitis

  • Kim, Mi Hye (Department of Convergence Korean Medical Science, Kyung Hee University College of Korean Medicine) ;
  • Choi, You Yeon (Department of Convergence Korean Medical Science, Kyung Hee University College of Korean Medicine) ;
  • Lee, Hye Ji (Department of Convergence Korean Medical Science, Kyung Hee University College of Korean Medicine) ;
  • Lee, Haesu (Department of Convergence Korean Medical Science, Kyung Hee University College of Korean Medicine) ;
  • Park, Jung-Chul (Department of Periodontology, Dankook University College of Dentistry) ;
  • Yang, Woong Mo (Department of Convergence Korean Medical Science, Kyung Hee University College of Korean Medicine)
  • Received : 2015.07.21
  • Accepted : 2015.08.16
  • Published : 2015.08.30
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Abstract

Purpose: The aim of this study was to investigate the therapeutic effects of a herbal formula, PerioH-035, containing Angelica sinensis, steamed Rehmannia glutinosa, Angelica dahurica, Cimicifuga heracleifolia, and Zanthoxylum piperitum on the periodontal breakdown in a well-established ligature-induced periodontitis model in rats. Methods: Sprague-Dawley rats were randomly assigned to 1 of 4 groups: NL (non-ligatured), L (ligatured), P1 (ligatured and treated with 1 mg/mL PerioH-035), P100 (ligatured and treated with 100 mg/mL PerioH-035). Periodontitis was induced by placing a ligature around the mandibular first molars. PerioH-035 was topically applied to both sides of the first molar for 2 weeks. The right side of the mandibles was retrieved for micro-computed tomography (CT) and methylene blue staining to analyze alveolar bone loss. The left side of the mandibles was histologically analyzed by TRAP and H&E staining. The MMP-9 mRNA level in gingival tissue was investigated by RT-PCR. Results: Alveolar bone resorption was significantly reduced in the PerioH-035-treated groups. The number of dense multi-nucleated cells found to be TRAP-positive by staining in the ligatured rats was markedly decreased by PerioH-035 application. In addition, periodontal tissue destruction, especially cementum demineralization, was ameliorated in the P1 and P100 groups. Moreover, gingival tissue from the PerioH-035-treated group showed a decrease in the MMP-9 mRNA level, resulting in recovery of collagen degradation. Conclusions: These results suggest that PerioH-035 has therapeutic effects on periodontitis, and thus, PerioH-035 shows promise as a treatment for periodontitis.

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References

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