Journal of the korean academy of Pediatric Dentistry (대한소아치과학회지)

Korean Academy of Pediatric Dentistry (대한소아치과학회)
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Antibacterial Properties of Pit and Fissure Sealant Containing S-PRG filler on Streptococcus mutans

S-PRG filler를 포함한 치면열구전색제의 Streptococcus mutans에 대한 항미생물 특성에 관한 연구

  • An, Jinseon (Department of Pediatric Dentistry and Oral Science Research Center, College of Dentistry, Gangneung-Wonju National University) ;
  • Park, Howon (Department of Pediatric Dentistry and Oral Science Research Center, College of Dentistry, Gangneung-Wonju National University) ;
  • Seo, Hyunwoo (Department of Pediatric Dentistry and Oral Science Research Center, College of Dentistry, Gangneung-Wonju National University) ;
  • Lee, Siyoung (Department of Oral Microbiology and Oral Science Research Center, College of Dentistry, Gangneung-Wonju National University)
  • 안진선 (강릉원주대학교 치과대학 소아치과학 교실 및 구강과학연구소) ;
  • 박호원 (강릉원주대학교 치과대학 소아치과학 교실 및 구강과학연구소) ;
  • 서현우 (강릉원주대학교 치과대학 소아치과학 교실 및 구강과학연구소) ;
  • 이시영 (강릉원주대학교 치과대학 미생물학 및 면역학교실 및 구강과학연구소)
  • Received : 2015.06.18
  • Accepted : 2015.07.17
  • Published : 2015.11.30
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Abstract

The purpose of this study was to evaluate the antibacterial properties of a sealant containing S-PRG filler compared to those of two contemporary commercial sealants to determine the inhibition of bacterial growth in broth culture and biofilm formation using the CDC Biofilm Reactor. The BeautiSealant containing S-PRG filler, the fluoride releasing Clinpro$^{TM}$ sealant, which are known to have higher antibacterial effects, and the non-fluoride releasing Concise$^{TM}$ sealant were selected for this study. A Streptococcus mutans culture in BHI broth without sealant served as a negative control in the planktonic growth inhibition test. As a result, bacterial growth was inhibited in all three sealant groups compared to that in the control. The Clinpro$^{TM}$ sealant showed a significantly reduced number of CFUs compared to those of the BeautiSealant and Concise$^{TM}$ sealants. However, no significant difference was detected between the BeautiSealant and Concise$^{TM}$ sealants. The Clinpro$^{TM}$ sealant significantly decreased biofilm formation compared to that by the BeautiSealant and Concise$^{TM}$ sealants. No significant difference was observed between the BeautiSealant and Concise$^{TM}$ sealants. In conclusion, the sealant containing S-PRG filler had a less potent anti-bacterial property and increased biofilm formation capacity compared to those of the fluoride releasing Clinpro$^{TM}$ sealant.

본 연구는 Planktonic growth inhibition test와 CDC Biofilm Reactor를 사용한 Biofilm assay를 통해 S-PRG filler를 함유하는 치면열구전색제의 S. mutans에 대한 항미생물 효과를 기존의 치면열구전색제와 비교하고자 하였다. S-PRG 필러를 함유하는 치면열구전색제인 BeautiSealant, 불소를 방출하는 치면열구전색제인 Clinpro$^{TM}$ sealant, 불소 미방출 치면열구전색제인 Concise$^{TM}$ sealant를 실험군으로 선정하였다. 성장억제평가를 위해 치면열구전색제를 사용하지 않은 군을 음성 대조군으로 설정하였으며, 3개의 실험군 모두 대조군보다 유의할 정도로 낮은 집락 형성 단위를 보였고, Clinpro$^{TM}$ sealant가 BeautiSealant와 Concise$^{TM}$ sealant보다 유의할 정도로 낮은 집락 형성 단위를 보였다. BeautiSealant와 Concise$^{TM}$ sealant 군간에는 유의한 차이가 관찰되지 않았다. 바이오 필름 평가에서도 Clinpro$^{TM}$ sealant군이 BeautiSealant와 Concise$^{TM}$ sealant군들에 비해 유의할 정도로 낮은 집락 형성을 보였으며, BeautiSealant와 Concise$^{TM}$ sealant 군간에 유의한 차이는 관찰되지 않았다. 본 연구 결과 S-PRG filler를 포함하는 치면열구전색제인 BeautiSealant는 기존의 불소방출 치면열구전색제에 비하여 낮은 항미생물 효과와 높은 바이오 필름 형성능을 보였다.

Keywords

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