DOI QR코드

DOI QR Code

부착조류 파판배양 시 Light Emitting Diodes (LEDs)의 적용

Adaptation of light emitting diode (LED) at culture on attachment plate of diatom

  • 배재현 (국립수산과학원 동해수산연구소 해역산업과) ;
  • 안희춘 (국립수산과학원 동해수산연구소 해역산업과) ;
  • 김미경 (국립수산과학원 동해수산연구소 해역산업과) ;
  • 박진철 (강릉원주대학교 해양자원육성학과) ;
  • 박흠기 (강릉원주대학교 해양자원육성학과) ;
  • 권오남 (강릉원주대학교 동해안생명과학연구소)
  • Bae, Jae-Hyun (Aquaculture Industry Division, East Sea Fisheries Research Institute, NFRDI) ;
  • An, Heui-Chun (Aquaculture Industry Division, East Sea Fisheries Research Institute, NFRDI) ;
  • Kim, Mi-Gyeong (Aquaculture Industry Division, East Sea Fisheries Research Institute, NFRDI) ;
  • Park, Jin-Chul (Department of Marine Bioscience Gangneung-Wonju National University) ;
  • Park, Heum-Gi (Department of Marine Bioscience Gangneung-Wonju National University) ;
  • Kwon, O-Nam (East Costal Life Science Institute Gangneung-Wonju National University)
  • 투고 : 2014.11.01
  • 심사 : 2014.11.26
  • 발행 : 2014.11.30

초록

We investigated biomass, diatom species and fucoxanthin contents as cell growth, fatty acid and amino acid contents as nutritional composition of diatoms attached on plate to confirm effects of light emitting diodes (LEDs) due to block off natural light. In the single LED irradiation, biomass showed significantly higher to $30.0{\pm}6.48mg/m^2$ in white LED than that of others (P<0.05). The dominate diatom species was Navicula cancellata. Their lipid contents showed significantly higher to $112.9{\pm}19.23ug/mg$ dry matter (DM) in control than that of others LEDs. But eicosapetaenoic acid (EPA) contents showed significantly higher to $3.3{\pm}0.62ug/mg$ DM than others, but not significantly differed with natural control light treatment (P<0.05). And total protein contents are higher in control and blue LED light than that of others, but essential amino acid contents showed significantly higher to $3.2{\pm}4.8%$ in control (P<0.05). In mixing light with natural and LED light, biomass showed $2.6{\pm}0.22mg/m^2$ in blue LED (P<0.05). Fatty acids contents were not significantly differed with all treatments. Amino acid contents showed to $11.0{\pm}0.33ug/mg$ DM in white LED (P<0.05), but not significantly differed with others LED lights (P>0.05). Therefore, we could suggest that irradiation of blue LED in natural light very benefit to diatom culture for larvae of sea cucumber and abalone and do on.

키워드

참고문헌

  1. Choi BR, Lim JH, Lee JK and Lee TY. 2013. Optimum conditions for cultivation of Chlorella sp. FC-21 using light emitting diodes. Korean J Chem Eng 30, 1614-1619. (doi:10.1007/s11814-013-0081-0)
  2. Das P, Lei W, Aziz SS and Obbard JP. 2011. Enhanced algae growth in both phototrophic and mixotrophic culture under blue light. Bio Technol 102, 3883-3887. (doi:10.1016/j.biortech.2010.11.102)
  3. Ducan DB. 1955. Multiple-range and mutiple F tests. Biometrics 11, 1-42. (doi:10.2307/3001478)
  4. Gordon N, Neori A, Lee J and Harpaz S. 2006. Effect of diatom diets on growth and survival of the abalone Haliotis discus hannai postlarvae. Aquaculture 252, 225-233. (doi:10.1016/j.aquaculture.2005.06.034)
  5. Jeong H, Lee J and Cha M. 2013. Energy efficient growth control of microalgae using photo methods. Renewable Energy 54, 161-165. (doi:10.1016/j.renene.2012.08.030)
  6. Katsuda T, Shimahara K, Shiraishi H, Yamagami K, Ranjbar R and Katoh S. 2006. Effect of flashing light from blue light emitting diodes on cell growth and astaxanthin production of Haematococcus pluvialis. J Biosci Bioeng 102, 442-446. (doi:10.1263/jbb.102.442)
  7. Lee CG and Palsson BO. 1994. High-density algal photobioreactors using light-emitting diodes. Biotechnol Bioeng 44(10), 1161-1167. (doi:10.1002/bit.260441002)
  8. Markou G. 2014. Effect of various colors of light-emitting diodes (LEDs) on the biomass composition of Arthrospira platensis cultivated in semi-continuous mode. Appl Biochem Biotechnol 172, 2758-2768. (doi:10.1007/s12010-014-0727-3)
  9. Mouget JL, Rosa P and Tremblin G. 2004. Acclimation of Haslea strearia to light of different spectral qualities-confirmation of 'chromatic adaptation' in diatoms. J Photochem Photobiol B 75, 1-11. (doi:10.1016/j.jphotobiol.2004.04.002)
  10. Mulo P, Sakurai I and Aro EM. 2012. Strategies for psbA gene expression in cyanobacteria, green algae and higher plants: form transcription to PSII repair. Biochim Biophys Acta 1817, 247-257. (doi: 10.1016/j.bbabio.2011.04.011)
  11. Park JC, Kwon ON, Hong SE, An HC, Bae JH, Park MS and Park HG. 2013. Changes in the growth and biochemical composition of Nannochloropsis sp. cultures using light-emitting diodes. Kor J Fish Aquat Sci 46, 259-265. https://doi.org/10.5657/KFAS.2013.0259
  12. Ruchin AB. 2004. Influence of colored light on growth rate of juveniles of fish. Fish Physiol Biochem 30, 175-178. (doi:10.1007/s10695-005-1263-4)
  13. Ruyters G. 1984. Effects of blue light on enzymes. Senger H, ed. Springer Verlag, Berlin, Germany, 283-301.
  14. Saha SK, McHugh E, Hayes J, Moane S, Walsh D and Murray P. 2013. Effect of various stress-regulatory factors on biomass and lipid production in microalga Haematococcus pluvialis. Bioresour Technol 128, 118-124. (doi:10.1016/j.biortech.2012.10.049)
  15. Sanchez-Saavedra MP and Voltolina D. 2006. The growth rate, biomass production and composition of Chaetoceros sp. grown with different light sources. Aquacult Eng 35, 161-165. (doi:10.1016/j.aquaeng.2005.12.001)
  16. Volpato GL and Barreto RE. 2001. Environmental blue light prevents stress in the fish Nile tilapia. Brazillian J Med Biol Res 34, 1041-1045. https://doi.org/10.1590/S0100-879X2001000800011
  17. Wang CY, Fu CC and Liu YC. 2007. Effects of using light emitting diodes on the cultivation of Spirulina platensis. Biochem Eng 37, 21-25. (doi:10.1016/j.bej.2007.03.004)
  18. Wellburn AR. 1994. The spectral determination of chlorophylls a and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. J Plant Physiol 144, 307-313. (doi:10.1016/S0176-1617(11)81192-2)
  19. Whatley FR. 1997. Changing views of photosynthesis. Further Mulestones in Biochemistry. Foundation of mordern biochemistry 3. Ord MG and Stocken LA, eds. Elsevier, Philadelphia, U.S.A., 23-66.
  20. Yoshioka M, Yago T, Yoshie-Stark Y, Arakawa H and Morinaga T. 2012. Effect of high frequency of intermittent light. Aquaculture 338-341, 111-117. (doi:10.1016/j.aquaculture.2012.01.005)