Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
권호 표지
DOI QR코드

DOI QR Code

Influence of Oxygen Consumption on Pregnancy Rates of Hanwoo Calves following Embryo Transfer

  • Kim, Hyun (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Bok, Nan-Hee (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Kim, Sung-Woo (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Do, Yoon-Jung (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Seong, Hwan-Hoo (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Kim, Jae-Hwan (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Kim, Dong Hun (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Kim, Min-Kyu (Department of Animal Science Graduate School, Chungnam National University) ;
  • Ko, Yeoung-Gyu (Animal Genetic Resources Station, National Institute of Animal Science, RDA)
  • Received : 2014.05.08
  • Accepted : 2014.09.22
  • Published : 2014.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Recently, several approaches have been used to measure the oxygen consumption rates of individual embryos, but relationship between oxygen consumption and pregnancy rates of Hanwoo following embryo transfer has not yet been reported. In this study, we investigated the correlation between oxygen consumption rate and pregnancy rates of Hanwoo embryo using a SECM. In addition to, the expression of apoptosis-related genes was determined using real-time PCR by extracting RNA according to the oxygen consumption of in vivo embryo. First, we found that the oxygen consumption significantly increased in blastocyst-stage embryos (blastocyst) compared to early blastocyst stage embryos, indicating that oxygen consumption reflects the embryo quality (Grade I). The oxygen consumption or GI blastocysts were significantly higher than those of GII blastocysts ($10.2{\times}10^{14}/mol\;s^{-1}$ versus $6.4{\times}10^{14}/mol\;s^{-1}$, p<0.05). Pregnant rate in recipient cow was 0, 60 and 80% in the transplantation of embryo with the oxygen consumption of below 10.0, 10.0~12.0 and over $12.0{\times}10^{14}/mol\;s^{-1}$, respectively. Apoptosis regulatory genes, Hsp-70.1 were significantly increased in over-10.0 group than below 10.0 group but in Caspase-3, Bax and P53 gene, there was no significant difference. In conclusion, These results suggest that measurement of oxygen consumption maybe help increase the pregnant rate of Hanwoo embryos.

Keywords

References

  1. Abe H, Shiku H, Yokoo M, Aoyagi S, Moriyasu S, Minami hashi A, Matsue T and Hoshi H. 2006. Evaluting the quality of individual embryos with a non-invasive and highly sensitive measurement of oxygen consumption and by scanning electrochemical microscopy. J. Reprod. Dev. 52:S55-S64.
  2. Barnett DK, Bavister BD. 1996. What is the relationship between the metabolism of preimplantation embryos and their developmental competence? Mol. Reprod. Dev. 43:105-33. https://doi.org/10.1002/(SICI)1098-2795(199601)43:1<105::AID-MRD13>3.0.CO;2-4
  3. Bergeron L, Perez GI, Macdonald G, Shi L, Sun Y, Jurisicova A, Varmuza S, Latham KE, Flaws JA, Salter JC, Hara H, Moskowitz MA, Li E, Greenberg A, Tilly JL and Yuan J. 1998. Defects in regulation of apoptosis in caspase-2 deficient mice. Genes Dev. 12:1304-1314. https://doi.org/10.1101/gad.12.9.1304
  4. Betts DH, King WA. 2001. Genetic regulation of embryo death and senescence. Theriogenology 55; 171-191. https://doi.org/10.1016/S0093-691X(00)00453-2
  5. Boiso I, Veiga A and Edwards RG. 2002. Fundamentals of human embryonic growth in vitro and the selection of high-quality embryos for transfer. Reproductive Biomedicine OnLine 5:328-350. https://doi.org/10.1016/S1472-6483(10)61841-X
  6. Donnay I. 2002. Assessment of Mammalian Embryo Quality: Invasive and Non-invasive Techniques. Dordrecht, The Netherlands: Kluwer Academic Publishers. 57-94.
  7. Eckert J, Pugh PA, Thompson JG, Niemann H and Tervit HR. 1998. Exogenous protein affects development competence and metabolic activity of bovine preimplantation embryos in vitro. Reproduction Fertility and Development 10:327- 332. https://doi.org/10.1071/R98077
  8. Farin PW, Britt JH, Shaw DW and Slenning BD. 1995. Agreement among evaluators of bovine embryos produced in vivo or in vitro. Theriogenology 44:339-349. https://doi.org/10.1016/0093-691X(95)00189-F
  9. Gjorret JO, Hiemke MK, Steph JD, Birthe A, Larsson L and Maddox-Hyttel P. 2003. Chronology of apoptosis in bovine embryos produced in vivo and in vitro. Biol. Reprod. 69: 1193-1200. https://doi.org/10.1095/biolreprod.102.013243
  10. Hasler JF. 1998. The current status of oocyte recovery, in vitro embryo production, and embryo transfer in domestic animals, with an emphasis on the bovine. Journal of Animal Science 76:52-74. https://doi.org/10.2527/1998.76suppl_352x
  11. Holm P, Shukri NN, Vajta G, Booth P, Bendixen C and Callesen H. 1998. Developmental kinetics of the first cell cycles of bovine in vitro produced embryos in relation to their in vitro viability and sex. Theriogenology 50:1285- 1299. https://doi.org/10.1016/S0093-691X(98)00227-1
  12. Holm P, Booth PJ, Schmidt MH, Greve T and Callesen H. 1999. High bovine blastocyst development in a static in vitro production system using SOFaa medium supplemented with sodium citrate and myo-inositol with or without serum-proteins. Theriogenology 52:683-700. https://doi.org/10.1016/S0093-691X(99)00162-4
  13. Houghton FD, Thompson JG, Kennedy CJ and Leese HJ. 1996. Oxygen consumption and energy metabolism of the early mouse embryo. Mol. Reprod. Dev. 44:476-485. https://doi.org/10.1002/(SICI)1098-2795(199608)44:4<476::AID-MRD7>3.0.CO;2-I
  14. Lane M and Gardner DK. 1996. Selection of viable mouse blastocysts prior to transfer using a metabolic criterion. Hum. Reprod. 11:1975-8. https://doi.org/10.1093/oxfordjournals.humrep.a019527
  15. Leese HJ. 2003. What does an embryo need? Human Fertility. 6:180-185. https://doi.org/10.1080/1464770312331369463
  16. Lopez AS, Larsen LH, Ramsing N, Lovendahl P, Raty M, Peippo J, Greve T and Callesen H. 2005. Respiration rates of individual bovine in vitro-produced embryos measured with a novel, non-invasive and highly sensitive microsensor system. Reproduction 130:669-679. https://doi.org/10.1530/rep.1.00703
  17. Magnusson C, Hillensjo T, Hamberger L and Nilsson L. 1986. Oxygen consumption by human oocytes and blastocysts grown in vitro. Human Reproduction 1:183-184. https://doi.org/10.1093/oxfordjournals.humrep.a136377
  18. Matsue T, Koike S and Uchida I. 1993. Microamperometric estimation of photosynthesis inhibition in a single algal protoplast. Biochem. Biophys. Res. Commun. 197:1283-1287. https://doi.org/10.1006/bbrc.1993.2616
  19. Merton S. 2002. Morphological Evaluation of Embryos in Domestic Animals. Dordrecht, The Netherlands: Kluwer Academic Publishers. 31-55.
  20. Overstrom EW, Burke PA, Hagopian SS and Selgraph JP. 1992. Blastocyst oxidative metabolism and embryo viability. J. Cell Biol. 107:607.
  21. Overstrom EW, Duby RT, Dobrinsky J, Roche JF and Boland MP. 1996. Viablity and oxydative metabolism of the bovine blastocyst. Theriogenology 37:269.
  22. Pomar FJ, Teerds KJ, Kidson A, Colenbrander B, Tharasanit T, Aguilar B and Roelen BA. 2005. Differences in the incidence of apoptosis between in vivo and in vitro produced blastocysts of farm animal species: a comparative study. Theriogenology 63:2254-2268. https://doi.org/10.1016/j.theriogenology.2004.10.015
  23. Shiku H, Shiraishi T, Ohya H, Matsue T, Abe H, Hoshi H and Kobayashi M. 2001. Oxygen consumption of single bovine embryos probed with scanning electrochemical microscopy. Anal. Chem. 73:3751-3758. https://doi.org/10.1021/ac010339j
  24. Shiku H, Shiraishi T, Aoyagi S, Utsumi Y, Matsudaira M, Abe H, Hoshi H, Kasai S, Ohya H and Matsue T. 2004. Respiration activity of single bovine embryos entrapped in a cone-shaped microwell monitored by scanning electrochemical microscopy. Analytica Chimica Acta 522:51-58. https://doi.org/10.1016/j.aca.2004.06.054
  25. Thompson JG, Partridge RJ, Houghton FD, Kennedy CJ, Pullar D, Wrathall AE and Leese HJ. 1995. Preliminary observations of the uptake of oxygen by day 7 bovine blastocysts. Theriogenology 43:3-37. https://doi.org/10.1016/0093-691X(94)00005-F
  26. Trimarchi JR, Liu L, Porterfield DM, Smith PJ and Keefe DL. 2000a. Oxidative phosphorylation-dependent and -independent oxygen consumption by individual preimplantation mouse embryos. Biology of Reproduction 62:1866-1874. https://doi.org/10.1095/biolreprod62.6.1866
  27. Trimarchi JR, Liu L, Porterfield DM, Smith PJ and Keefe DL. 2000b. A non-invasive method for measuring preimplantation embryo physiology. Zygote 8:15-24. https://doi.org/10.1017/S0967199400000782
  28. Thrimarchi JR, Liu L, Smith PJS and Keefe DL. 2000. Noninvasive measurement of potassium efflux as an early indicator of cell death in mouse embryos. Biol. Reprod. 63:851-857. https://doi.org/10.1095/biolreprod63.3.851
  29. Yang M, Rajamahendran R. 2002. Expression of Bcl-2 and Bax proteins in relation to quality of bovine oocytes and embryos produced in vitro. Anim. Reprod. Sci. 15:159-169.
  30. Ziebe S, Petersen K, Lindenberg S, Andersen AG, Gabrielsen A and Andersen AN. 1997. Embryo morphology or cleavage stage: how to select the embryos for transfer after in-vitro fertilization. Hum. Reprod. 12:1545-9. https://doi.org/10.1093/humrep/12.7.1545