YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Effect of Chamomile Flower Extract on Septic Arthritis due to Candida albicans

카모마일 꽃 추출물의 Candida albicans 기인성 감염성 관절염에 대한 효과

  • Kim, Jeonghyeon (College of Pharmacy, Dongduk Women's University) ;
  • Kim, Songyi (National Institute of Food and Drug Safety Evaluation) ;
  • Hong, Yuna (College of Pharmacy, Seoul National University) ;
  • Kim, Yeong Shik (College of Pharmacy, Seoul National University) ;
  • Han, Yongmoon (College of Pharmacy, Dongduk Women's University)
  • 김정현 (동덕여자대학교 약학대학 면역.미생물학교실) ;
  • 김송이 (식품의약품안전평가원 의약품심사부) ;
  • 홍유나 (서울대학교 약학대학 천연물연구소) ;
  • 김영식 (서울대학교 약학대학 천연물연구소) ;
  • 한용문 (동덕여자대학교 약학대학 면역.미생물학교실)
  • Received : 2014.06.30
  • Accepted : 2014.09.26
  • Published : 2014.10.31
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Abstract

In the present studies, we examined effect of chamomile flowers extract (CH-Ex), which has traditionally been used as antiphlogistics in Europe for many centuries, against Candida albicans-caused septic arthritis. Candida albicans is a major etiological agent among fungal septic arthritis. This effect was investigated in a murine model of the septic arthritis. That is, mice that were given an emulsion form of C. albicans cell wall (CACW) via footpad route were treated intraperitoneally with the CH-Ex for 3 times every 3 days. Degrees of the footpad-swellings were measured with dial gauger. Data showed that the CH-Ex resulted in the reduction of swelling. For instance, at Day 9 when swelling reached the highest peak, there was up to app. 60% reduction of edema in mice injected with the CH-Ex, compared to that of the control mice that received no treatment (P<0.05). This therapeutic anti-arthritic activity appeared to be mediated by inhibitions of NO (nitric oxide) production from activated RAW264.7 macrophages and proliferation of Con A-treated T lymphocytes. Analysis by HPLC revealed that the CH-Ex contained eight polyphenolic compounds including chlorogenic acid (CRA) and rutin. We have reported the CRA and rutin respectively have the anti-arthritic activity. This correlation implicates that CRA and rutin in the CH-Ex may be responsible for the activity. Combined all together, the CH-Ex has anti-arthritic activity against C. albicans-caused septic arthritis, possibly by inhibiting NO production and proliferation of T cells. This activity seems to be contributed by, at least, CRA and rutin among the compounds in the CH-Ex.

Keywords

References

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