YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Anti-allergic and Anti-inflammatory Effect of Leonurus sibiricus Seed Ethyl Acetate Fractions

충울자 EtOAc 분획의 항알러지 및 항염증 효과

  • Jung, Yujung (Natural Products Research Center, KIST Gangneung Institute) ;
  • Jeon, Youngsic (Natural Products Research Center, KIST Gangneung Institute) ;
  • Kim, Hyung Ja (Molecular Recognition Research Center, KIST) ;
  • Kang, Ki Sung (College of Korean Medicine, Gachon University) ;
  • Kim, Yong Kee (College of Pharmacy, Sookmyung Women's University) ;
  • Kim, Su-Nam (Natural Products Research Center, KIST Gangneung Institute)
  • 정유정 (한국과학기술연구원 천연물융합연구센터) ;
  • 전영식 (한국과학기술연구원 천연물융합연구센터) ;
  • 김형자 (한국과학기술연구원 분자인식연구센터) ;
  • 강기성 (가천대학교 한의과대학) ;
  • 김용기 (숙명여자대학교 약학대학) ;
  • 김수남 (한국과학기술연구원 천연물융합연구센터)
  • Received : 2014.06.10
  • Accepted : 2014.09.04
  • Published : 2014.10.31
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Abstract

In this study, we investigate anti-allergic and anti-inflammatory effects of Leonurus sibiricus seed (LSS) extract in basophilic leukemia RBL-2H3 cells. To identify anti-allergic actions of LSS, the degranulation was evaluated in IgE and DNP-BSA stimulated RBL-2H3 cells. At the concentration of $100{\mu}g/ml$ of methanol (MeOH) extract and Methylene chloride (MC) and Ethyl acetate (EtOAc) fractions, the degranulation was significantly inhibited 16.7%, 16.7% and 27.9% respectively. And then, to assess anti-inflammatory effects of LSS, IL-4 and IL-13 mRNA level were detected in PMA/ionomycin (PI)-induced RBL-2H3 cells and cell proliferation and IL-4 mRNA level in isolated splenocytes from Balb/c mice. LSS MeOH extract and MC and EtOAc fractions significantly decreased the level of IL-4 and IL-13 mRNA in PI-induced RBL-2H3 cells and showed inhibitory effects on cell proliferation and expression of IL-4 mRNA level in mouse splenocytes. Taken together, these results suggest that LSS has potential anti-allergic and anti-inflammatory effects and EtOAc fraction is the most effective in regulating immune responses.

Keywords

References

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